Downregulation of GABAB receptors in the spinal cord dorsal horn in diabetic neuropathy
Diabetic neuropathic pain is a common clinical problem and remains difficult to treat with classic analgesics. Spinal dorsal horn neurons are important in mediating nociceptive signaling, and the hyperactivity of these neurons is critical in diabetic neuropathy. In this study, we determined the GABA...
Gespeichert in:
| Veröffentlicht in: | Neuroscience letters 2011-02, Vol.490 (2), p.112-115 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 115 |
|---|---|
| container_issue | 2 |
| container_start_page | 112 |
| container_title | Neuroscience letters |
| container_volume | 490 |
| creator | WANG, Xiu-Li QI ZHANG ZHANG, Ying-Ze LIU, Yan-Tao RUI DONG QIU JUN WANG GUO, Yue-Xian |
| description | Diabetic neuropathic pain is a common clinical problem and remains difficult to treat with classic analgesics. Spinal dorsal horn neurons are important in mediating nociceptive signaling, and the hyperactivity of these neurons is critical in diabetic neuropathy. In this study, we determined the GABA(B) receptor expression level in dorsal horn neurons in streptozotocin (STZ)-induced diabetes in rats by using reverse-transcription polymerase chain reaction (RT-PCR) and western blot analyses. Mean blood glucose concentrations were significantly higher and the paw withdrawal threshold was significantly lower in STZ-treated rats than in saline-treated rats. Immunohistochemical staining showed that the GABA(B) receptor was extensively expressed in the spinal dorsal horn neurons. The GABA(B1) mRNA level decreased in a time-dependent manner in STZ-treated rats compared with saline-treated controls. Furthermore, the protein expression level revealed by western blot analysis was lower in STZ-treated rats than in saline-treated rats. These data suggest that GABA(B) receptors are downregulated in the spinal dorsal horn in this model of STZ-induced diabetic neuropathic pain. The reduction of GABA(B) expression may contribute to the hyperactivity of spinal dorsal horn neurons and diabetic neuropathic pain. |
| doi_str_mv | 10.1016/j.neulet.2010.12.038 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_848823254</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>848823254</sourcerecordid><originalsourceid>FETCH-LOGICAL-c317t-39b8deb049d0685961dd18c9d15ca582f35ea4e26fb29538b678eea000dac3f03</originalsourceid><addsrcrecordid>eNpFkUtPwzAMgCMEYmPwDxDKBXFqyaOP9LgNGEiTuIA4Rmnisk5dU5JWaP-eVBtwSmR_ju0vCF1TElNCs_tt3MLQQB8zMoZYTLg4QVMqchblRc5O0ZRwkkS8SMgEXXi_JYSkNE3O0YRRKhJB8in6eLDfrYPPoVF9bVtsK7yaL-YL7EBD11vncd3ifgPYd3WrGqytM9iEeLhvrGvHtKlVCX2tcZjI2U71m_0lOqtU4-HqeM7Q-9Pj2_I5Wr-uXpbzdaQ5zfswXCkMlCQpDMlEWmTUGCp0YWiqVSpYxVNQCbCsKlmRclFmuQBQYROjNK8In6G7w7uds18D-F7uaq-haVQLdvAyrCkYZ2kSyORAame9d1DJztU75faSEjkalVt5MCpHo5IyGYyGsptjg6Hcgfkr-lUYgNsjoLxWTeVUq2v_z_HwITzP-A-WkoEK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>848823254</pqid></control><display><type>article</type><title>Downregulation of GABAB receptors in the spinal cord dorsal horn in diabetic neuropathy</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>WANG, Xiu-Li ; QI ZHANG ; ZHANG, Ying-Ze ; LIU, Yan-Tao ; RUI DONG ; QIU JUN WANG ; GUO, Yue-Xian</creator><creatorcontrib>WANG, Xiu-Li ; QI ZHANG ; ZHANG, Ying-Ze ; LIU, Yan-Tao ; RUI DONG ; QIU JUN WANG ; GUO, Yue-Xian</creatorcontrib><description>Diabetic neuropathic pain is a common clinical problem and remains difficult to treat with classic analgesics. Spinal dorsal horn neurons are important in mediating nociceptive signaling, and the hyperactivity of these neurons is critical in diabetic neuropathy. In this study, we determined the GABA(B) receptor expression level in dorsal horn neurons in streptozotocin (STZ)-induced diabetes in rats by using reverse-transcription polymerase chain reaction (RT-PCR) and western blot analyses. Mean blood glucose concentrations were significantly higher and the paw withdrawal threshold was significantly lower in STZ-treated rats than in saline-treated rats. Immunohistochemical staining showed that the GABA(B) receptor was extensively expressed in the spinal dorsal horn neurons. The GABA(B1) mRNA level decreased in a time-dependent manner in STZ-treated rats compared with saline-treated controls. Furthermore, the protein expression level revealed by western blot analysis was lower in STZ-treated rats than in saline-treated rats. These data suggest that GABA(B) receptors are downregulated in the spinal dorsal horn in this model of STZ-induced diabetic neuropathic pain. The reduction of GABA(B) expression may contribute to the hyperactivity of spinal dorsal horn neurons and diabetic neuropathic pain.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2010.12.038</identifier><identifier>PMID: 21184807</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier</publisher><subject>Animals ; Biological and medical sciences ; Diabetes Mellitus, Experimental - metabolism ; Diabetes Mellitus, Experimental - pathology ; Diabetes. Impaired glucose tolerance ; Diabetic Neuropathies - metabolism ; Diabetic Neuropathies - pathology ; Down-Regulation - physiology ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Fundamental and applied biological sciences. Psychology ; Male ; Medical sciences ; Pain Measurement - methods ; Posterior Horn Cells - metabolism ; Posterior Horn Cells - pathology ; Rats ; Rats, Sprague-Dawley ; Receptors, GABA-B - biosynthesis ; Receptors, GABA-B - metabolism ; RNA, Messenger - biosynthesis ; Spinal Cord - metabolism ; Spinal Cord - pathology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 2011-02, Vol.490 (2), p.112-115</ispartof><rights>2015 INIST-CNRS</rights><rights>Crown Copyright © 2010. Published by Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c317t-39b8deb049d0685961dd18c9d15ca582f35ea4e26fb29538b678eea000dac3f03</citedby><cites>FETCH-LOGICAL-c317t-39b8deb049d0685961dd18c9d15ca582f35ea4e26fb29538b678eea000dac3f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23872376$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21184807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WANG, Xiu-Li</creatorcontrib><creatorcontrib>QI ZHANG</creatorcontrib><creatorcontrib>ZHANG, Ying-Ze</creatorcontrib><creatorcontrib>LIU, Yan-Tao</creatorcontrib><creatorcontrib>RUI DONG</creatorcontrib><creatorcontrib>QIU JUN WANG</creatorcontrib><creatorcontrib>GUO, Yue-Xian</creatorcontrib><title>Downregulation of GABAB receptors in the spinal cord dorsal horn in diabetic neuropathy</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Diabetic neuropathic pain is a common clinical problem and remains difficult to treat with classic analgesics. Spinal dorsal horn neurons are important in mediating nociceptive signaling, and the hyperactivity of these neurons is critical in diabetic neuropathy. In this study, we determined the GABA(B) receptor expression level in dorsal horn neurons in streptozotocin (STZ)-induced diabetes in rats by using reverse-transcription polymerase chain reaction (RT-PCR) and western blot analyses. Mean blood glucose concentrations were significantly higher and the paw withdrawal threshold was significantly lower in STZ-treated rats than in saline-treated rats. Immunohistochemical staining showed that the GABA(B) receptor was extensively expressed in the spinal dorsal horn neurons. The GABA(B1) mRNA level decreased in a time-dependent manner in STZ-treated rats compared with saline-treated controls. Furthermore, the protein expression level revealed by western blot analysis was lower in STZ-treated rats than in saline-treated rats. These data suggest that GABA(B) receptors are downregulated in the spinal dorsal horn in this model of STZ-induced diabetic neuropathic pain. The reduction of GABA(B) expression may contribute to the hyperactivity of spinal dorsal horn neurons and diabetic neuropathic pain.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Diabetic Neuropathies - metabolism</subject><subject>Diabetic Neuropathies - pathology</subject><subject>Down-Regulation - physiology</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Etiopathogenesis. Screening. Investigations. Target tissue resistance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Pain Measurement - methods</subject><subject>Posterior Horn Cells - metabolism</subject><subject>Posterior Horn Cells - pathology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, GABA-B - biosynthesis</subject><subject>Receptors, GABA-B - metabolism</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Spinal Cord - metabolism</subject><subject>Spinal Cord - pathology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkUtPwzAMgCMEYmPwDxDKBXFqyaOP9LgNGEiTuIA4Rmnisk5dU5JWaP-eVBtwSmR_ju0vCF1TElNCs_tt3MLQQB8zMoZYTLg4QVMqchblRc5O0ZRwkkS8SMgEXXi_JYSkNE3O0YRRKhJB8in6eLDfrYPPoVF9bVtsK7yaL-YL7EBD11vncd3ifgPYd3WrGqytM9iEeLhvrGvHtKlVCX2tcZjI2U71m_0lOqtU4-HqeM7Q-9Pj2_I5Wr-uXpbzdaQ5zfswXCkMlCQpDMlEWmTUGCp0YWiqVSpYxVNQCbCsKlmRclFmuQBQYROjNK8In6G7w7uds18D-F7uaq-haVQLdvAyrCkYZ2kSyORAame9d1DJztU75faSEjkalVt5MCpHo5IyGYyGsptjg6Hcgfkr-lUYgNsjoLxWTeVUq2v_z_HwITzP-A-WkoEK</recordid><startdate>20110225</startdate><enddate>20110225</enddate><creator>WANG, Xiu-Li</creator><creator>QI ZHANG</creator><creator>ZHANG, Ying-Ze</creator><creator>LIU, Yan-Tao</creator><creator>RUI DONG</creator><creator>QIU JUN WANG</creator><creator>GUO, Yue-Xian</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110225</creationdate><title>Downregulation of GABAB receptors in the spinal cord dorsal horn in diabetic neuropathy</title><author>WANG, Xiu-Li ; QI ZHANG ; ZHANG, Ying-Ze ; LIU, Yan-Tao ; RUI DONG ; QIU JUN WANG ; GUO, Yue-Xian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c317t-39b8deb049d0685961dd18c9d15ca582f35ea4e26fb29538b678eea000dac3f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetes Mellitus, Experimental - pathology</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Diabetic Neuropathies - metabolism</topic><topic>Diabetic Neuropathies - pathology</topic><topic>Down-Regulation - physiology</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Etiopathogenesis. Screening. Investigations. Target tissue resistance</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Pain Measurement - methods</topic><topic>Posterior Horn Cells - metabolism</topic><topic>Posterior Horn Cells - pathology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, GABA-B - biosynthesis</topic><topic>Receptors, GABA-B - metabolism</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Spinal Cord - metabolism</topic><topic>Spinal Cord - pathology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WANG, Xiu-Li</creatorcontrib><creatorcontrib>QI ZHANG</creatorcontrib><creatorcontrib>ZHANG, Ying-Ze</creatorcontrib><creatorcontrib>LIU, Yan-Tao</creatorcontrib><creatorcontrib>RUI DONG</creatorcontrib><creatorcontrib>QIU JUN WANG</creatorcontrib><creatorcontrib>GUO, Yue-Xian</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WANG, Xiu-Li</au><au>QI ZHANG</au><au>ZHANG, Ying-Ze</au><au>LIU, Yan-Tao</au><au>RUI DONG</au><au>QIU JUN WANG</au><au>GUO, Yue-Xian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Downregulation of GABAB receptors in the spinal cord dorsal horn in diabetic neuropathy</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2011-02-25</date><risdate>2011</risdate><volume>490</volume><issue>2</issue><spage>112</spage><epage>115</epage><pages>112-115</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Diabetic neuropathic pain is a common clinical problem and remains difficult to treat with classic analgesics. Spinal dorsal horn neurons are important in mediating nociceptive signaling, and the hyperactivity of these neurons is critical in diabetic neuropathy. In this study, we determined the GABA(B) receptor expression level in dorsal horn neurons in streptozotocin (STZ)-induced diabetes in rats by using reverse-transcription polymerase chain reaction (RT-PCR) and western blot analyses. Mean blood glucose concentrations were significantly higher and the paw withdrawal threshold was significantly lower in STZ-treated rats than in saline-treated rats. Immunohistochemical staining showed that the GABA(B) receptor was extensively expressed in the spinal dorsal horn neurons. The GABA(B1) mRNA level decreased in a time-dependent manner in STZ-treated rats compared with saline-treated controls. Furthermore, the protein expression level revealed by western blot analysis was lower in STZ-treated rats than in saline-treated rats. These data suggest that GABA(B) receptors are downregulated in the spinal dorsal horn in this model of STZ-induced diabetic neuropathic pain. The reduction of GABA(B) expression may contribute to the hyperactivity of spinal dorsal horn neurons and diabetic neuropathic pain.</abstract><cop>Shannon</cop><pub>Elsevier</pub><pmid>21184807</pmid><doi>10.1016/j.neulet.2010.12.038</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 2011-02, Vol.490 (2), p.112-115 |
| issn | 0304-3940 1872-7972 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_848823254 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Biological and medical sciences Diabetes Mellitus, Experimental - metabolism Diabetes Mellitus, Experimental - pathology Diabetes. Impaired glucose tolerance Diabetic Neuropathies - metabolism Diabetic Neuropathies - pathology Down-Regulation - physiology Endocrine pancreas. Apud cells (diseases) Endocrinopathies Etiopathogenesis. Screening. Investigations. Target tissue resistance Fundamental and applied biological sciences. Psychology Male Medical sciences Pain Measurement - methods Posterior Horn Cells - metabolism Posterior Horn Cells - pathology Rats Rats, Sprague-Dawley Receptors, GABA-B - biosynthesis Receptors, GABA-B - metabolism RNA, Messenger - biosynthesis Spinal Cord - metabolism Spinal Cord - pathology Vertebrates: nervous system and sense organs |
| title | Downregulation of GABAB receptors in the spinal cord dorsal horn in diabetic neuropathy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T02%3A43%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Downregulation%20of%20GABAB%20receptors%20in%20the%20spinal%20cord%20dorsal%20horn%20in%20diabetic%20neuropathy&rft.jtitle=Neuroscience%20letters&rft.au=WANG,%20Xiu-Li&rft.date=2011-02-25&rft.volume=490&rft.issue=2&rft.spage=112&rft.epage=115&rft.pages=112-115&rft.issn=0304-3940&rft.eissn=1872-7972&rft.coden=NELED5&rft_id=info:doi/10.1016/j.neulet.2010.12.038&rft_dat=%3Cproquest_cross%3E848823254%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=848823254&rft_id=info:pmid/21184807&rfr_iscdi=true |