Isolation and characterization of DNA from fixed cells and tissues
DNA was isolated from cultured mammalian cells, liver tissue and Escherichia coli after treatment with the common laboratory fixatives for various periods of time. The cultured cells included the mouse cell strain LM and the Chinese hamster cell strain Don C. Liver tissue was obtained from young adu...
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| Veröffentlicht in: | Experimental cell research 1968-04, Vol.50 (1), p.47-53 |
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| container_title | Experimental cell research |
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| creator | Arrighi, Frances E. Bergendahl, Janet Mandel, Manley |
| description | DNA was isolated from cultured mammalian cells, liver tissue and
Escherichia coli after treatment with the common laboratory fixatives for various periods of time. The cultured cells included the mouse cell strain LM and the Chinese hamster cell strain Don C. Liver tissue was obtained from young adult Chinese hamsters. Ultraviolet spectral absorbance ratios, CsCl buoyant density determinations, melting curves and hyperchromicity were used to characterize the DNA samples.
Recovery of “normal” DNA was excellent with most fixatives, but not when formalin was used. Formalin fixation interfered with the isolation procedure, and when DNA was extracted the buoyant densities in CsCl were significantly different from the DNA extracted from unfixed materials, but the
T
m
values were not different. |
| doi_str_mv | 10.1016/0014-4827(68)90392-3 |
| format | Article |
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Escherichia coli after treatment with the common laboratory fixatives for various periods of time. The cultured cells included the mouse cell strain LM and the Chinese hamster cell strain Don C. Liver tissue was obtained from young adult Chinese hamsters. Ultraviolet spectral absorbance ratios, CsCl buoyant density determinations, melting curves and hyperchromicity were used to characterize the DNA samples.
Recovery of “normal” DNA was excellent with most fixatives, but not when formalin was used. Formalin fixation interfered with the isolation procedure, and when DNA was extracted the buoyant densities in CsCl were significantly different from the DNA extracted from unfixed materials, but the
T
m
values were not different.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(68)90392-3</identifier><identifier>PMID: 4870421</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>1-Propanol - pharmacology ; Animals ; Centrifugation, Density Gradient ; Cricetinae ; Culture Techniques ; Densitometry ; DNA - analysis ; Escherichia coli - analysis ; Ethanol - pharmacology ; Formaldehyde - pharmacology ; Histological Techniques ; Hot Temperature ; Liver - analysis ; Methanol - pharmacology ; Methods ; Mice ; Nucleic Acid Denaturation ; Tissue Preservation</subject><ispartof>Experimental cell research, 1968-04, Vol.50 (1), p.47-53</ispartof><rights>1968</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-2cea2614ef600bc7c6ef29056d451e81679424938f83201dfc20663c1b0597e3</citedby><cites>FETCH-LOGICAL-c357t-2cea2614ef600bc7c6ef29056d451e81679424938f83201dfc20663c1b0597e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014482768903923$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4870421$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arrighi, Frances E.</creatorcontrib><creatorcontrib>Bergendahl, Janet</creatorcontrib><creatorcontrib>Mandel, Manley</creatorcontrib><title>Isolation and characterization of DNA from fixed cells and tissues</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>DNA was isolated from cultured mammalian cells, liver tissue and
Escherichia coli after treatment with the common laboratory fixatives for various periods of time. The cultured cells included the mouse cell strain LM and the Chinese hamster cell strain Don C. Liver tissue was obtained from young adult Chinese hamsters. Ultraviolet spectral absorbance ratios, CsCl buoyant density determinations, melting curves and hyperchromicity were used to characterize the DNA samples.
Recovery of “normal” DNA was excellent with most fixatives, but not when formalin was used. Formalin fixation interfered with the isolation procedure, and when DNA was extracted the buoyant densities in CsCl were significantly different from the DNA extracted from unfixed materials, but the
T
m
values were not different.</description><subject>1-Propanol - pharmacology</subject><subject>Animals</subject><subject>Centrifugation, Density Gradient</subject><subject>Cricetinae</subject><subject>Culture Techniques</subject><subject>Densitometry</subject><subject>DNA - analysis</subject><subject>Escherichia coli - analysis</subject><subject>Ethanol - pharmacology</subject><subject>Formaldehyde - pharmacology</subject><subject>Histological Techniques</subject><subject>Hot Temperature</subject><subject>Liver - analysis</subject><subject>Methanol - pharmacology</subject><subject>Methods</subject><subject>Mice</subject><subject>Nucleic Acid Denaturation</subject><subject>Tissue Preservation</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EKqXwByBlhWARGD_iOBukUl6VKth0b6XOWBjlUewUAV9P0lRdshpp5t6ZO4eQcwo3FKi8BaAiFoqlV1JdZ8AzFvMDMqaQQcwEY4dkvJcck5MQPgBAKSpHZCRUCoLRMbmfh6bMW9fUUV4XkXnPfW5a9O53aDY2enidRtY3VWTdN3YSLMuwFbcuhA2GU3Jk8zLg2a5OyPLpcTl7iRdvz_PZdBEbnqRtzAzmTFKBVgKsTGokWpZBIguRUOxipZlgIuPKKs6AFtYwkJIbuoIkS5FPyOWwdu2bz-5sqysX-jB5jc0maCVUksnOMSFiEBrfhODR6rV3Ve5_NAXdk9M9Ft1j0VLpLTnNO9vFbv9mVWGxN-1QdfO7YY7dj18OvQ7GYW2wcB5Nq4vG_X_gDyLye1c</recordid><startdate>196804</startdate><enddate>196804</enddate><creator>Arrighi, Frances E.</creator><creator>Bergendahl, Janet</creator><creator>Mandel, Manley</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196804</creationdate><title>Isolation and characterization of DNA from fixed cells and tissues</title><author>Arrighi, Frances E. ; Bergendahl, Janet ; Mandel, Manley</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-2cea2614ef600bc7c6ef29056d451e81679424938f83201dfc20663c1b0597e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>1-Propanol - pharmacology</topic><topic>Animals</topic><topic>Centrifugation, Density Gradient</topic><topic>Cricetinae</topic><topic>Culture Techniques</topic><topic>Densitometry</topic><topic>DNA - analysis</topic><topic>Escherichia coli - analysis</topic><topic>Ethanol - pharmacology</topic><topic>Formaldehyde - pharmacology</topic><topic>Histological Techniques</topic><topic>Hot Temperature</topic><topic>Liver - analysis</topic><topic>Methanol - pharmacology</topic><topic>Methods</topic><topic>Mice</topic><topic>Nucleic Acid Denaturation</topic><topic>Tissue Preservation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arrighi, Frances E.</creatorcontrib><creatorcontrib>Bergendahl, Janet</creatorcontrib><creatorcontrib>Mandel, Manley</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arrighi, Frances E.</au><au>Bergendahl, Janet</au><au>Mandel, Manley</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of DNA from fixed cells and tissues</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1968-04</date><risdate>1968</risdate><volume>50</volume><issue>1</issue><spage>47</spage><epage>53</epage><pages>47-53</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>DNA was isolated from cultured mammalian cells, liver tissue and
Escherichia coli after treatment with the common laboratory fixatives for various periods of time. The cultured cells included the mouse cell strain LM and the Chinese hamster cell strain Don C. Liver tissue was obtained from young adult Chinese hamsters. Ultraviolet spectral absorbance ratios, CsCl buoyant density determinations, melting curves and hyperchromicity were used to characterize the DNA samples.
Recovery of “normal” DNA was excellent with most fixatives, but not when formalin was used. Formalin fixation interfered with the isolation procedure, and when DNA was extracted the buoyant densities in CsCl were significantly different from the DNA extracted from unfixed materials, but the
T
m
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| ispartof | Experimental cell research, 1968-04, Vol.50 (1), p.47-53 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 1-Propanol - pharmacology Animals Centrifugation, Density Gradient Cricetinae Culture Techniques Densitometry DNA - analysis Escherichia coli - analysis Ethanol - pharmacology Formaldehyde - pharmacology Histological Techniques Hot Temperature Liver - analysis Methanol - pharmacology Methods Mice Nucleic Acid Denaturation Tissue Preservation |
| title | Isolation and characterization of DNA from fixed cells and tissues |
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