Autophagy of prostate cancer PC-3 cells under the induction of oridonin

Autophagy of prostate cancer PC-3 cells under the induction of oridonin

To investigate the mechanism of oridonin (ORI)-induced autophagy in prostate cancer PC-3 cells. The PC-3 cells cultured in vitro were treated with ORI. The inhibitory ratio of oridonin In PC-3 cells was assayed by MTT. The ultrastructural cellular changes were observed under light microscope, scanni...

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Veröffentlicht in:Zhong hua yi xue za zhi 2010-11, Vol.90 (42), p.2984-2988
Hauptverfasser: Ye, Li-hong, Chen, Yong-liang, Tao, Shui-xiang, Jiang, Xiao-qiang, Li, Wang-jian, Qian, Wei-liang, He, Jian-song
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Sprache:chi
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Apoptosis - drug effects
Autophagy - drug effects
Cell Line, Tumor
Cell Proliferation - drug effects
Diterpenes, Kaurane - pharmacology
Humans
Male
Prostatic Neoplasms - pathology
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container_end_page 2988
container_issue 42
container_start_page 2984
container_title Zhong hua yi xue za zhi
container_volume 90
creator Ye, Li-hong
Chen, Yong-liang
Tao, Shui-xiang
Jiang, Xiao-qiang
Li, Wang-jian
Qian, Wei-liang
He, Jian-song
description To investigate the mechanism of oridonin (ORI)-induced autophagy in prostate cancer PC-3 cells. The PC-3 cells cultured in vitro were treated with ORI. The inhibitory ratio of oridonin In PC-3 cells was assayed by MTT. The ultrastructural cellular changes were observed under light microscope, scanning electron microscope (SEM) and transmission electron microscope (TEM). AO staining was used to observe the acidic vesicular organelles (AVOs). The level of MAP1-LC3 was detected by Western blot and reverse transcriptase polymerase chain reaction (RT-PCR) used to detect the level of mRNA of beclin 1. After oridonin treatment, the proliferation of PC-3 cells was inhibited significantly in a concentration and time-dependent manner. The SEM examination revealed cellular shrinkage and the disappearance of surface microvilli after ORI treatment. And on the TEM examination, the nuclei exhibited chromatin condensation and the appearance of a large number of autophagosome with double-membrane structure in cytoplasm. AO staining showed the existence of AVOs. Simultaneously, the expressions of autophagy-related proteins, MAP-LC3 and the mRNA level of beclin 1 were elevated by ORI. The autophagy inhibitor 3-MA reversed the elevation of beclin 1 mRNA. The growth of PC-3 cells is inhibited. And cellular is induced by oridonin with a potential anti-tumor effect.
doi_str_mv 10.3760/ema.j.issn.0376-2491.2010.42.010
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subjects Apoptosis - drug effects
Autophagy - drug effects
Cell Line, Tumor
Cell Proliferation - drug effects
Diterpenes, Kaurane - pharmacology
Humans
Male
Prostatic Neoplasms - pathology
title Autophagy of prostate cancer PC-3 cells under the induction of oridonin
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