Force spectroscopy of an elastic peptide: Effect of D2O and temperature on persistence length

This study explores the mechanical unfolding of elastic protein analogues as a function of temperature, in both H2O and D2O, using atomic force microscopy (AFM) force spectroscopy in a specially constructed AFM liquid cell. This represents the first time that the effect of D2O on protein flexibility...

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Veröffentlicht in:	Microscopy research and technique 2011-02, Vol.74 (2), p.170-176
Hauptverfasser:	Haward, Simon J., Shewry, Peter R., Marsh, Justin, Miles, Mervyn J., Mc Master, Terence J.
Format:	Artikel
Sprache:	eng
Schlagworte:	AFM Elasticity isotopic substitution Microscopy, Atomic Force Peptides - chemistry Peptides - genetics Peptides - metabolism Protein Folding single molecule stretching Temperature Water
Online-Zugang:	Volltext
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