Oral Epithelial Cells are Activated via TRP Channels
Transient receptor potential (TRP) ion channels are critical contributors to the perception of various environmental stimuli. Although the oral cavity is the access point for various food types, the expression of TRP channels in oral mucosa remains unknown. We hypothesized that the oral epithelium i...
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| Veröffentlicht in: | Journal of dental research 2011-02, Vol.90 (2), p.163-167 |
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| creator | Wang, B. Danjo, A. Kajiya, H. Okabe, K. Kido, M.A. |
| description | Transient receptor potential (TRP) ion channels are critical contributors to the perception of various environmental stimuli. Although the oral cavity is the access point for various food types, the expression of TRP channels in oral mucosa remains unknown. We hypothesized that the oral epithelium itself may participate in sensing thermal, mechanical, and chemical conditions. The expression profiles of TRP channels exhibited regional differences among the buccal, palatal, and tongue epithelia. Changes in elevated intracellular Ca2+ concentration ([Ca2+]i) in oral epithelial cells were found after stimulation of the TRP channels with capsaicin, camphor, 4α-phorbol-12,13 didecanoate (4α-PDD), 2-aminoethoxydiphenyl borate (2-APB), and menthol. These increases in Ca2+ appeared dependent on the TRP channels, because [Ca2+]i suppression was observed after the addition of the TRPV channel antagonist ruthenium red. These results demonstrate that the oral epithelia express various TRP channels and may have functional roles in sensory activities, together with neurons. |
| doi_str_mv | 10.1177/0022034510385459 |
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Although the oral cavity is the access point for various food types, the expression of TRP channels in oral mucosa remains unknown. We hypothesized that the oral epithelium itself may participate in sensing thermal, mechanical, and chemical conditions. The expression profiles of TRP channels exhibited regional differences among the buccal, palatal, and tongue epithelia. Changes in elevated intracellular Ca2+ concentration ([Ca2+]i) in oral epithelial cells were found after stimulation of the TRP channels with capsaicin, camphor, 4α-phorbol-12,13 didecanoate (4α-PDD), 2-aminoethoxydiphenyl borate (2-APB), and menthol. These increases in Ca2+ appeared dependent on the TRP channels, because [Ca2+]i suppression was observed after the addition of the TRPV channel antagonist ruthenium red. These results demonstrate that the oral epithelia express various TRP channels and may have functional roles in sensory activities, together with neurons.</description><identifier>ISSN: 0022-0345</identifier><identifier>EISSN: 1544-0591</identifier><identifier>DOI: 10.1177/0022034510385459</identifier><identifier>PMID: 21149857</identifier><identifier>CODEN: JDREAF</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Biological and medical sciences ; Calcium - metabolism ; Cells, Cultured ; Dentistry ; Epithelial Cells - metabolism ; Epithelial Cells - physiology ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Male ; Mouth Mucosa - cytology ; Mouth Mucosa - metabolism ; Mouth Mucosa - physiology ; Rats ; Rats, Sprague-Dawley ; Sensation - physiology ; Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors ; Transient Receptor Potential Channels - biosynthesis ; Transient Receptor Potential Channels - genetics ; Trigeminal Ganglion - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of dental research, 2011-02, Vol.90 (2), p.163-167</ispartof><rights>2011 International & American Associations for Dental Research</rights><rights>2015 INIST-CNRS</rights><rights>SAGE Publications © Feb 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-e57aefb12022472c55ea8ce9e3d00b535d9188c0e9203ca55a184ad3ddafcd103</citedby><cites>FETCH-LOGICAL-c459t-e57aefb12022472c55ea8ce9e3d00b535d9188c0e9203ca55a184ad3ddafcd103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0022034510385459$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0022034510385459$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23932152$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21149857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, B.</creatorcontrib><creatorcontrib>Danjo, A.</creatorcontrib><creatorcontrib>Kajiya, H.</creatorcontrib><creatorcontrib>Okabe, K.</creatorcontrib><creatorcontrib>Kido, M.A.</creatorcontrib><title>Oral Epithelial Cells are Activated via TRP Channels</title><title>Journal of dental research</title><addtitle>J Dent Res</addtitle><description>Transient receptor potential (TRP) ion channels are critical contributors to the perception of various environmental stimuli. Although the oral cavity is the access point for various food types, the expression of TRP channels in oral mucosa remains unknown. We hypothesized that the oral epithelium itself may participate in sensing thermal, mechanical, and chemical conditions. The expression profiles of TRP channels exhibited regional differences among the buccal, palatal, and tongue epithelia. Changes in elevated intracellular Ca2+ concentration ([Ca2+]i) in oral epithelial cells were found after stimulation of the TRP channels with capsaicin, camphor, 4α-phorbol-12,13 didecanoate (4α-PDD), 2-aminoethoxydiphenyl borate (2-APB), and menthol. These increases in Ca2+ appeared dependent on the TRP channels, because [Ca2+]i suppression was observed after the addition of the TRPV channel antagonist ruthenium red. These results demonstrate that the oral epithelia express various TRP channels and may have functional roles in sensory activities, together with neurons.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Cells, Cultured</subject><subject>Dentistry</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Male</subject><subject>Mouth Mucosa - cytology</subject><subject>Mouth Mucosa - metabolism</subject><subject>Mouth Mucosa - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sensation - physiology</subject><subject>Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors</subject><subject>Transient Receptor Potential Channels - biosynthesis</subject><subject>Transient Receptor Potential Channels - genetics</subject><subject>Trigeminal Ganglion - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-0345</issn><issn>1544-0591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1Lw0AQxRdRbK3ePUkQxFN0P5vdYwn1AwoVqecw3UxsSprU3aTgf--WVgsFTzMwvzfz5hFyzegDY0nySCnnVEjFqNBKKnNC-kxJGVNl2Cnpb8fxdt4jF94vKWWGa3FOepwxabRK-kROHVTReF22C6zK0KZYVT4Ch9HItuUGWsyjTQnR7P0tShdQ11j5S3JWQOXxal8H5ONpPEtf4sn0-TUdTWIbvLQxqgSwmDMebMiEW6UQtEWDIqd0roTKDdPaUjThCQtKAdMScpHnUNg8_DQg97u9a9d8dejbbFV6GwxCjU3nMy21CNLhMJC3R-Sy6VwdzGVaUcMkVzJAdAdZ13jvsMjWrlyB-84YzbZ5Zsd5BsnNfm83X2H-J_gNMAB3ewC8hapwUNvSHzhhBGeKBy7ecR4-8WDu38M_vV-Guw</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Wang, B.</creator><creator>Danjo, A.</creator><creator>Kajiya, H.</creator><creator>Okabe, K.</creator><creator>Kido, M.A.</creator><general>SAGE Publications</general><general>International Association for Dental Research</general><general>SAGE PUBLICATIONS, INC</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>U9A</scope><scope>7X8</scope></search><sort><creationdate>20110201</creationdate><title>Oral Epithelial Cells are Activated via TRP Channels</title><author>Wang, B. ; Danjo, A. ; Kajiya, H. ; Okabe, K. ; Kido, M.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-e57aefb12022472c55ea8ce9e3d00b535d9188c0e9203ca55a184ad3ddafcd103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Cells, Cultured</topic><topic>Dentistry</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Male</topic><topic>Mouth Mucosa - cytology</topic><topic>Mouth Mucosa - metabolism</topic><topic>Mouth Mucosa - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sensation - physiology</topic><topic>Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors</topic><topic>Transient Receptor Potential Channels - biosynthesis</topic><topic>Transient Receptor Potential Channels - genetics</topic><topic>Trigeminal Ganglion - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, B.</creatorcontrib><creatorcontrib>Danjo, A.</creatorcontrib><creatorcontrib>Kajiya, H.</creatorcontrib><creatorcontrib>Okabe, K.</creatorcontrib><creatorcontrib>Kido, M.A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, B.</au><au>Danjo, A.</au><au>Kajiya, H.</au><au>Okabe, K.</au><au>Kido, M.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oral Epithelial Cells are Activated via TRP Channels</atitle><jtitle>Journal of dental research</jtitle><addtitle>J Dent Res</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>90</volume><issue>2</issue><spage>163</spage><epage>167</epage><pages>163-167</pages><issn>0022-0345</issn><eissn>1544-0591</eissn><coden>JDREAF</coden><abstract>Transient receptor potential (TRP) ion channels are critical contributors to the perception of various environmental stimuli. Although the oral cavity is the access point for various food types, the expression of TRP channels in oral mucosa remains unknown. We hypothesized that the oral epithelium itself may participate in sensing thermal, mechanical, and chemical conditions. The expression profiles of TRP channels exhibited regional differences among the buccal, palatal, and tongue epithelia. Changes in elevated intracellular Ca2+ concentration ([Ca2+]i) in oral epithelial cells were found after stimulation of the TRP channels with capsaicin, camphor, 4α-phorbol-12,13 didecanoate (4α-PDD), 2-aminoethoxydiphenyl borate (2-APB), and menthol. These increases in Ca2+ appeared dependent on the TRP channels, because [Ca2+]i suppression was observed after the addition of the TRPV channel antagonist ruthenium red. These results demonstrate that the oral epithelia express various TRP channels and may have functional roles in sensory activities, together with neurons.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>21149857</pmid><doi>10.1177/0022034510385459</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Calcium - metabolism Cells, Cultured Dentistry Epithelial Cells - metabolism Epithelial Cells - physiology Fundamental and applied biological sciences. Psychology Gene Expression Male Mouth Mucosa - cytology Mouth Mucosa - metabolism Mouth Mucosa - physiology Rats Rats, Sprague-Dawley Sensation - physiology Somesthesis and somesthetic pathways (proprioception, exteroception, nociception) interoception electrolocation. Sensory receptors Transient Receptor Potential Channels - biosynthesis Transient Receptor Potential Channels - genetics Trigeminal Ganglion - metabolism Vertebrates: nervous system and sense organs |
| title | Oral Epithelial Cells are Activated via TRP Channels |
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