A Modification of the Hemolytic Plaque Assay for Use with Protein Antigens

A method is presented which allows the in vitro enumeration of cells producing antibody against a variety of protein antigens. The proteins are covalently linked to red blood cells by means of a carbodiimide reagent. The concentrations of protein required for the plaque assay are greater than those...

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Veröffentlicht in:	The Journal of immunology (1950) 1968-01, Vol.100 (1), p.133-137
Hauptverfasser:	Golub, Edward S, Mishell, Robert I, Weigle, William O, Dutton, Richard W
Format:	Artikel
Sprache:	eng
Schlagworte:	Agar Animals Antibody Formation Antigen-Antibody Reactions Antigens - pharmacology Erythrocytes Ferritins - pharmacology gamma-Globulins - pharmacology Hemolysis Humans Imides Immunoassay Indicators and Reagents Kinetics Proteins - pharmacology Serum Albumin, Bovine - pharmacology Thyroglobulin - pharmacology Transferrin - pharmacology
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