Relationship of Molecular Size of rIn:rCn (Poly I:C) to Induction of Interferon and Host Resistance
Summary Preparations of high molecular weight rIn:rCn were exposed to sonic radiation. A decrease in the average molecular weight of the polynucleotide complexes after treatment was accompanied by an exponential decrease in viscosity of the preparation, an increase in the sensitivity of the polynucl...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1970-12, Vol.135 (3), p.911-916 |
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| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 135 |
| creator | Lampson, George P. Field, A. Kirk Tytell, Alfred A. Nemes, Marjorie M. Hilleman, Maurice R. |
| description | Summary
Preparations of high molecular weight rIn:rCn were exposed to sonic radiation. A decrease in the average molecular weight of the polynucleotide complexes after treatment was accompanied by an exponential decrease in viscosity of the preparation, an increase in the sensitivity of the polynucleotide complex to degradation by pancreatic ribonuclease, a decrease in the thermal transition midpoint, and a decrease in the hyperchromicity observed during thermal transition.
The lower molecular weight rIn:rCn complexes were tested for their capacity to induce interferon in rabbits and resistance against VSV infection in cell culture and PVM infection in mice. Sonic-treated preparations of rIn:rCn reduced to an average molecular weight of approximately 4.6 × 105 were markedly reduced in capacity to protect mice against PVM infection, compared with untreated rIn:rCn preparations containing complexes having an average molecular weight of approximately 7.8 × 106. The capacity of sonic-treated rIn:rCn to induce production of rabbit interferon and resistance to virus infection in cell culture was more resistant to molecular weight reduction. Significant decrease in activity was observed in rIn:rCn fractions with approximate molecular weights less than 1.2 × 105. |
| doi_str_mv | 10.3181/00379727-135-35169 |
| format | Article |
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Preparations of high molecular weight rIn:rCn were exposed to sonic radiation. A decrease in the average molecular weight of the polynucleotide complexes after treatment was accompanied by an exponential decrease in viscosity of the preparation, an increase in the sensitivity of the polynucleotide complex to degradation by pancreatic ribonuclease, a decrease in the thermal transition midpoint, and a decrease in the hyperchromicity observed during thermal transition.
The lower molecular weight rIn:rCn complexes were tested for their capacity to induce interferon in rabbits and resistance against VSV infection in cell culture and PVM infection in mice. Sonic-treated preparations of rIn:rCn reduced to an average molecular weight of approximately 4.6 × 105 were markedly reduced in capacity to protect mice against PVM infection, compared with untreated rIn:rCn preparations containing complexes having an average molecular weight of approximately 7.8 × 106. The capacity of sonic-treated rIn:rCn to induce production of rabbit interferon and resistance to virus infection in cell culture was more resistant to molecular weight reduction. Significant decrease in activity was observed in rIn:rCn fractions with approximate molecular weights less than 1.2 × 105.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-135-35169</identifier><identifier>PMID: 4321038</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Chemical Phenomena ; Chemistry ; Chromatography ; Culture Techniques ; Cytosine Nucleotides ; Interferons - biosynthesis ; Mice ; Molecular Weight ; Nucleosides ; Optics and Photonics ; Pneumonia, Viral - immunology ; Polynucleotides ; Rabbits ; Ribonucleases - pharmacology ; Ultrasonics ; Vesicular stomatitis Indiana virus - immunology ; Viscosity</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1970-12, Vol.135 (3), p.911-916</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-55907fe2e974df6324924db195c8ff8e263667175f2bef632c02831ddd6969833</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4321038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lampson, George P.</creatorcontrib><creatorcontrib>Field, A. Kirk</creatorcontrib><creatorcontrib>Tytell, Alfred A.</creatorcontrib><creatorcontrib>Nemes, Marjorie M.</creatorcontrib><creatorcontrib>Hilleman, Maurice R.</creatorcontrib><title>Relationship of Molecular Size of rIn:rCn (Poly I:C) to Induction of Interferon and Host Resistance</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
Preparations of high molecular weight rIn:rCn were exposed to sonic radiation. A decrease in the average molecular weight of the polynucleotide complexes after treatment was accompanied by an exponential decrease in viscosity of the preparation, an increase in the sensitivity of the polynucleotide complex to degradation by pancreatic ribonuclease, a decrease in the thermal transition midpoint, and a decrease in the hyperchromicity observed during thermal transition.
The lower molecular weight rIn:rCn complexes were tested for their capacity to induce interferon in rabbits and resistance against VSV infection in cell culture and PVM infection in mice. Sonic-treated preparations of rIn:rCn reduced to an average molecular weight of approximately 4.6 × 105 were markedly reduced in capacity to protect mice against PVM infection, compared with untreated rIn:rCn preparations containing complexes having an average molecular weight of approximately 7.8 × 106. The capacity of sonic-treated rIn:rCn to induce production of rabbit interferon and resistance to virus infection in cell culture was more resistant to molecular weight reduction. Significant decrease in activity was observed in rIn:rCn fractions with approximate molecular weights less than 1.2 × 105.</description><subject>Animals</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Chromatography</subject><subject>Culture Techniques</subject><subject>Cytosine Nucleotides</subject><subject>Interferons - biosynthesis</subject><subject>Mice</subject><subject>Molecular Weight</subject><subject>Nucleosides</subject><subject>Optics and Photonics</subject><subject>Pneumonia, Viral - immunology</subject><subject>Polynucleotides</subject><subject>Rabbits</subject><subject>Ribonucleases - pharmacology</subject><subject>Ultrasonics</subject><subject>Vesicular stomatitis Indiana virus - immunology</subject><subject>Viscosity</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAUhYMoOj7-gCBkJbqo5tEkjTsZfBQUxcc6dNIbrXSSMWkX46-3dUaXri73nu8cuAehQ0rOOC3oOSFcacVURrnIuKBSb6AJFeMitd5EkxHIRmIH7ab0QQgVislttJ1zRgkvJsg-QVt1TfDpvVng4PB9aMH2bRXxc_MF4yWW_iJOPT55DO0SlxfTU9wFXPq6t6NxRErfQXQQh63yNb4NqcNPkJrUVd7CPtpyVZvgYD330Ov11cv0Nrt7uCmnl3eZzUneZUJoohww0CqvneQs1yyvZ1QLWzhXAJNcSkWVcGwGo24JKzit61pqqQvO99DxKncRw2cPqTPzJllo28pD6JMpciWFKsQAshVoY0gpgjOL2MyruDSUmLFZ89usGZo1P80OpqN1ej-bQ_1nWVc56OcrPVVvYD5CH_3w7H-J31W7f6Q</recordid><startdate>197012</startdate><enddate>197012</enddate><creator>Lampson, George P.</creator><creator>Field, A. Kirk</creator><creator>Tytell, Alfred A.</creator><creator>Nemes, Marjorie M.</creator><creator>Hilleman, Maurice R.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197012</creationdate><title>Relationship of Molecular Size of rIn:rCn (Poly I:C) to Induction of Interferon and Host Resistance</title><author>Lampson, George P. ; Field, A. Kirk ; Tytell, Alfred A. ; Nemes, Marjorie M. ; Hilleman, Maurice R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-55907fe2e974df6324924db195c8ff8e263667175f2bef632c02831ddd6969833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Animals</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Chromatography</topic><topic>Culture Techniques</topic><topic>Cytosine Nucleotides</topic><topic>Interferons - biosynthesis</topic><topic>Mice</topic><topic>Molecular Weight</topic><topic>Nucleosides</topic><topic>Optics and Photonics</topic><topic>Pneumonia, Viral - immunology</topic><topic>Polynucleotides</topic><topic>Rabbits</topic><topic>Ribonucleases - pharmacology</topic><topic>Ultrasonics</topic><topic>Vesicular stomatitis Indiana virus - immunology</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lampson, George P.</creatorcontrib><creatorcontrib>Field, A. Kirk</creatorcontrib><creatorcontrib>Tytell, Alfred A.</creatorcontrib><creatorcontrib>Nemes, Marjorie M.</creatorcontrib><creatorcontrib>Hilleman, Maurice R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lampson, George P.</au><au>Field, A. Kirk</au><au>Tytell, Alfred A.</au><au>Nemes, Marjorie M.</au><au>Hilleman, Maurice R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship of Molecular Size of rIn:rCn (Poly I:C) to Induction of Interferon and Host Resistance</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1970-12</date><risdate>1970</risdate><volume>135</volume><issue>3</issue><spage>911</spage><epage>916</epage><pages>911-916</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
Preparations of high molecular weight rIn:rCn were exposed to sonic radiation. A decrease in the average molecular weight of the polynucleotide complexes after treatment was accompanied by an exponential decrease in viscosity of the preparation, an increase in the sensitivity of the polynucleotide complex to degradation by pancreatic ribonuclease, a decrease in the thermal transition midpoint, and a decrease in the hyperchromicity observed during thermal transition.
The lower molecular weight rIn:rCn complexes were tested for their capacity to induce interferon in rabbits and resistance against VSV infection in cell culture and PVM infection in mice. Sonic-treated preparations of rIn:rCn reduced to an average molecular weight of approximately 4.6 × 105 were markedly reduced in capacity to protect mice against PVM infection, compared with untreated rIn:rCn preparations containing complexes having an average molecular weight of approximately 7.8 × 106. The capacity of sonic-treated rIn:rCn to induce production of rabbit interferon and resistance to virus infection in cell culture was more resistant to molecular weight reduction. Significant decrease in activity was observed in rIn:rCn fractions with approximate molecular weights less than 1.2 × 105.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>4321038</pmid><doi>10.3181/00379727-135-35169</doi><tpages>6</tpages></addata></record> |
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| ispartof | Experimental biology and medicine (Maywood, N.J.), 1970-12, Vol.135 (3), p.911-916 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Chemical Phenomena Chemistry Chromatography Culture Techniques Cytosine Nucleotides Interferons - biosynthesis Mice Molecular Weight Nucleosides Optics and Photonics Pneumonia, Viral - immunology Polynucleotides Rabbits Ribonucleases - pharmacology Ultrasonics Vesicular stomatitis Indiana virus - immunology Viscosity |
| title | Relationship of Molecular Size of rIn:rCn (Poly I:C) to Induction of Interferon and Host Resistance |
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