Development of an ELISA system for tick‐borne encephalitis virus infection in rodents
ABSTRACT Tick‐borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. An epizootiological survey of wild rodents is effective to detect TBE virus‐endemic areas; however, limited serological diagnostic methods are available to detect anti‐TBE virus antibodies in wil...
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| Veröffentlicht in: | Microbiology and immunology 2011-02, Vol.55 (2), p.100-107 |
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| creator | Ikawa‐Yoshida, Ayae Yoshii, Kentaro Kuwahara, Kazue Obara, Mayumi Kariwa, Hiroaki Takashima, Ikuo |
| description | ABSTRACT
Tick‐borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. An epizootiological survey of wild rodents is effective to detect TBE virus‐endemic areas; however, limited serological diagnostic methods are available to detect anti‐TBE virus antibodies in wild rodents. In this study, ELISAs for the detection of rodent antibodies against the TBE virus were developed using two recombinant proteins, domain III of the E protein (EdIII) and subviral particles (SPs), as the antigens. As compared with the neutralization test, the ELISA using EdIII had 77.1% sensitivity and 80.0% specificity, and the ELISA using SPs had 91.4% sensitivity and 100% specificity. Furthermore, when the ELISAs were applied to the epizootiological survey in the TBE virus‐endemic area, both of the ELISAs was able to detect wild rodents with TBE virus‐specific antibodies. This is the first study to show that ELISAs using recombinant antigens can be safe and useful in the detection of TBE virus‐infected wild rodents in epizootiological research. |
| doi_str_mv | 10.1111/j.1348-0421.2010.00296.x |
| format | Article |
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Tick‐borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. An epizootiological survey of wild rodents is effective to detect TBE virus‐endemic areas; however, limited serological diagnostic methods are available to detect anti‐TBE virus antibodies in wild rodents. In this study, ELISAs for the detection of rodent antibodies against the TBE virus were developed using two recombinant proteins, domain III of the E protein (EdIII) and subviral particles (SPs), as the antigens. As compared with the neutralization test, the ELISA using EdIII had 77.1% sensitivity and 80.0% specificity, and the ELISA using SPs had 91.4% sensitivity and 100% specificity. Furthermore, when the ELISAs were applied to the epizootiological survey in the TBE virus‐endemic area, both of the ELISAs was able to detect wild rodents with TBE virus‐specific antibodies. This is the first study to show that ELISAs using recombinant antigens can be safe and useful in the detection of TBE virus‐infected wild rodents in epizootiological research.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.2010.00296.x</identifier><identifier>PMID: 21204948</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Animals ; Antibodies, Viral - analysis ; Antibodies, Viral - immunology ; Arvicolinae ; Cell Line ; Cricetinae ; E protein ; Encephalitis Viruses, Tick-Borne - immunology ; Encephalitis Viruses, Tick-Borne - isolation & purification ; Encephalitis Viruses, Tick-Borne - physiology ; Encephalitis, Tick-Borne - diagnosis ; Encephalitis, Tick-Borne - immunology ; Encephalitis, Tick-Borne - veterinary ; Encephalitis, Tick-Borne - virology ; Enzyme-Linked Immunosorbent Assay - methods ; epizootiology ; Murinae ; Rodent Diseases - diagnosis ; Rodent Diseases - immunology ; Rodent Diseases - virology ; rodents ; subviral particles ; tick‐borne encephalitis</subject><ispartof>Microbiology and immunology, 2011-02, Vol.55 (2), p.100-107</ispartof><rights>2011 The Societies and Blackwell Publishing Asia Pty Ltd</rights><rights>2011 The Societies and Blackwell Publishing Asia Pty Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1348-0421.2010.00296.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1348-0421.2010.00296.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21204948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ikawa‐Yoshida, Ayae</creatorcontrib><creatorcontrib>Yoshii, Kentaro</creatorcontrib><creatorcontrib>Kuwahara, Kazue</creatorcontrib><creatorcontrib>Obara, Mayumi</creatorcontrib><creatorcontrib>Kariwa, Hiroaki</creatorcontrib><creatorcontrib>Takashima, Ikuo</creatorcontrib><title>Development of an ELISA system for tick‐borne encephalitis virus infection in rodents</title><title>Microbiology and immunology</title><addtitle>Microbiol Immunol</addtitle><description>ABSTRACT
Tick‐borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. An epizootiological survey of wild rodents is effective to detect TBE virus‐endemic areas; however, limited serological diagnostic methods are available to detect anti‐TBE virus antibodies in wild rodents. In this study, ELISAs for the detection of rodent antibodies against the TBE virus were developed using two recombinant proteins, domain III of the E protein (EdIII) and subviral particles (SPs), as the antigens. As compared with the neutralization test, the ELISA using EdIII had 77.1% sensitivity and 80.0% specificity, and the ELISA using SPs had 91.4% sensitivity and 100% specificity. Furthermore, when the ELISAs were applied to the epizootiological survey in the TBE virus‐endemic area, both of the ELISAs was able to detect wild rodents with TBE virus‐specific antibodies. This is the first study to show that ELISAs using recombinant antigens can be safe and useful in the detection of TBE virus‐infected wild rodents in epizootiological research.</description><subject>Animals</subject><subject>Antibodies, Viral - analysis</subject><subject>Antibodies, Viral - immunology</subject><subject>Arvicolinae</subject><subject>Cell Line</subject><subject>Cricetinae</subject><subject>E protein</subject><subject>Encephalitis Viruses, Tick-Borne - immunology</subject><subject>Encephalitis Viruses, Tick-Borne - isolation & purification</subject><subject>Encephalitis Viruses, Tick-Borne - physiology</subject><subject>Encephalitis, Tick-Borne - diagnosis</subject><subject>Encephalitis, Tick-Borne - immunology</subject><subject>Encephalitis, Tick-Borne - veterinary</subject><subject>Encephalitis, Tick-Borne - virology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>epizootiology</subject><subject>Murinae</subject><subject>Rodent Diseases - diagnosis</subject><subject>Rodent Diseases - immunology</subject><subject>Rodent Diseases - virology</subject><subject>rodents</subject><subject>subviral particles</subject><subject>tick‐borne encephalitis</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtOwzAQQC0EoqVwBeQdqwTHnySW2FSlQKVWLACxtPIZC5f8iNPS7jgCZ-QkOLR0NjOaeRrNPIRwQPzAxfXSDxiPPcJp4FPiuoRQGfqbIzQ8DI7RkLBYeCIkZIDOrF06KKIxP0UDGlDCJY-H6PUW1lDUTQlVh2uNkwpP57OnMbZb20GJdd3izmTvP1_fad1WgKHKoHlLCtMZi9emXVlsKg1ZZ-rKVbitc7fKnqMTnRQWLvZ5hF7ups-TB2_-eD-bjOdeE0gWehEwISSLNGM5C2MmCWWpzLXmPHAHghBURlTHkoY01ilIGiWQZhwikfOQMjZCV7u9TVt_rMB2qjQ2g6JIKqhXVsU8ElK4bx15uSdXaQm5alpTJu1W_btwwM0O-DQFbA_zgKjeuVqqXq3q1areufpzrjZqMVu4gv0Co0J0Xg</recordid><startdate>201102</startdate><enddate>201102</enddate><creator>Ikawa‐Yoshida, Ayae</creator><creator>Yoshii, Kentaro</creator><creator>Kuwahara, Kazue</creator><creator>Obara, Mayumi</creator><creator>Kariwa, Hiroaki</creator><creator>Takashima, Ikuo</creator><general>Blackwell Publishing Asia</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201102</creationdate><title>Development of an ELISA system for tick‐borne encephalitis virus infection in rodents</title><author>Ikawa‐Yoshida, Ayae ; Yoshii, Kentaro ; Kuwahara, Kazue ; Obara, Mayumi ; Kariwa, Hiroaki ; Takashima, Ikuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1936-7e355937f33d36839023b9dff441494e552972f892628fbe927aebc4e75d46233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antibodies, Viral - analysis</topic><topic>Antibodies, Viral - immunology</topic><topic>Arvicolinae</topic><topic>Cell Line</topic><topic>Cricetinae</topic><topic>E protein</topic><topic>Encephalitis Viruses, Tick-Borne - immunology</topic><topic>Encephalitis Viruses, Tick-Borne - isolation & purification</topic><topic>Encephalitis Viruses, Tick-Borne - physiology</topic><topic>Encephalitis, Tick-Borne - diagnosis</topic><topic>Encephalitis, Tick-Borne - immunology</topic><topic>Encephalitis, Tick-Borne - veterinary</topic><topic>Encephalitis, Tick-Borne - virology</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>epizootiology</topic><topic>Murinae</topic><topic>Rodent Diseases - diagnosis</topic><topic>Rodent Diseases - immunology</topic><topic>Rodent Diseases - virology</topic><topic>rodents</topic><topic>subviral particles</topic><topic>tick‐borne encephalitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ikawa‐Yoshida, Ayae</creatorcontrib><creatorcontrib>Yoshii, Kentaro</creatorcontrib><creatorcontrib>Kuwahara, Kazue</creatorcontrib><creatorcontrib>Obara, Mayumi</creatorcontrib><creatorcontrib>Kariwa, Hiroaki</creatorcontrib><creatorcontrib>Takashima, Ikuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ikawa‐Yoshida, Ayae</au><au>Yoshii, Kentaro</au><au>Kuwahara, Kazue</au><au>Obara, Mayumi</au><au>Kariwa, Hiroaki</au><au>Takashima, Ikuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an ELISA system for tick‐borne encephalitis virus infection in rodents</atitle><jtitle>Microbiology and immunology</jtitle><addtitle>Microbiol Immunol</addtitle><date>2011-02</date><risdate>2011</risdate><volume>55</volume><issue>2</issue><spage>100</spage><epage>107</epage><pages>100-107</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><abstract>ABSTRACT
Tick‐borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. An epizootiological survey of wild rodents is effective to detect TBE virus‐endemic areas; however, limited serological diagnostic methods are available to detect anti‐TBE virus antibodies in wild rodents. In this study, ELISAs for the detection of rodent antibodies against the TBE virus were developed using two recombinant proteins, domain III of the E protein (EdIII) and subviral particles (SPs), as the antigens. As compared with the neutralization test, the ELISA using EdIII had 77.1% sensitivity and 80.0% specificity, and the ELISA using SPs had 91.4% sensitivity and 100% specificity. Furthermore, when the ELISAs were applied to the epizootiological survey in the TBE virus‐endemic area, both of the ELISAs was able to detect wild rodents with TBE virus‐specific antibodies. This is the first study to show that ELISAs using recombinant antigens can be safe and useful in the detection of TBE virus‐infected wild rodents in epizootiological research.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>21204948</pmid><doi>10.1111/j.1348-0421.2010.00296.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antibodies, Viral - analysis Antibodies, Viral - immunology Arvicolinae Cell Line Cricetinae E protein Encephalitis Viruses, Tick-Borne - immunology Encephalitis Viruses, Tick-Borne - isolation & purification Encephalitis Viruses, Tick-Borne - physiology Encephalitis, Tick-Borne - diagnosis Encephalitis, Tick-Borne - immunology Encephalitis, Tick-Borne - veterinary Encephalitis, Tick-Borne - virology Enzyme-Linked Immunosorbent Assay - methods epizootiology Murinae Rodent Diseases - diagnosis Rodent Diseases - immunology Rodent Diseases - virology rodents subviral particles tick‐borne encephalitis |
| title | Development of an ELISA system for tick‐borne encephalitis virus infection in rodents |
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