Complete estrogen receptor blocker ICI182,780 promotes the proliferation of vascular smooth muscle cells
ICI182,780 is used in adjuvant therapies of breast cancer. As a complete estrogen receptor （ER） blocker, ICI182,780 may antagonize the effects of estrogen on the cardiovascular system. Estrogen inhibits the proliferation of vascular smooth muscle cells （VSMCs）, which is one of the mechanisms that es...
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| Veröffentlicht in: | Acta biochimica et biophysica Sinica 2011-02, Vol.43 (2), p.118-123 |
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| creator | He, Hong Yang, Falin Wang, Xin Zeng, Xianwei Hu, Qin Huang, Jinghuan Feng, Jinbo |
| description | ICI182,780 is used in adjuvant therapies of breast cancer. As a complete estrogen receptor （ER） blocker, ICI182,780 may antagonize the effects of estrogen on the cardiovascular system. Estrogen inhibits the proliferation of vascular smooth muscle cells （VSMCs）, which is one of the mechanisms that estrogen can exert cardioprotective effects. In the present study, to assess the effects of ICI182,780 on the proliferation of VSMCs, we cultured VSMCs isolated from rat aorta with or without the ER antagonist ICI182,780. The results of 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay, bromodeoxyuridine incorporation assay, viable cell count, immunochemicai staining for proliferating cell nuclear antigen （PCNA）, and S-phase ratio determined by flow cytometry revealed a remarkable proliferation of VSMCs after ICI182,780 treatment. ICI182,780 significantly enhanced cell growth in a dose-dependent manner （10-8-10-s M）. Furthermore, the number of PCNA-positive cells and the S-phase progression of VSMCs increased after treatment with ICI182,780. Reverse transcriptase-polymerase chain reaction （RT-PCR） and western blot analysis showed that the mRNA and protein level of cyclin D1 in VSMCs increased under the treatment of ICI182,780. These data suggested that ICI182,780 can promote the growth of VSMCs, which might produce some adverse effects on the cardiovascular system. |
| doi_str_mv | 10.1093/abbs/gmq119 |
| format | Article |
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As a complete estrogen receptor （ER） blocker, ICI182,780 may antagonize the effects of estrogen on the cardiovascular system. Estrogen inhibits the proliferation of vascular smooth muscle cells （VSMCs）, which is one of the mechanisms that estrogen can exert cardioprotective effects. In the present study, to assess the effects of ICI182,780 on the proliferation of VSMCs, we cultured VSMCs isolated from rat aorta with or without the ER antagonist ICI182,780. The results of 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay, bromodeoxyuridine incorporation assay, viable cell count, immunochemicai staining for proliferating cell nuclear antigen （PCNA）, and S-phase ratio determined by flow cytometry revealed a remarkable proliferation of VSMCs after ICI182,780 treatment. ICI182,780 significantly enhanced cell growth in a dose-dependent manner （10-8-10-s M）. Furthermore, the number of PCNA-positive cells and the S-phase progression of VSMCs increased after treatment with ICI182,780. Reverse transcriptase-polymerase chain reaction （RT-PCR） and western blot analysis showed that the mRNA and protein level of cyclin D1 in VSMCs increased under the treatment of ICI182,780. These data suggested that ICI182,780 can promote the growth of VSMCs, which might produce some adverse effects on the cardiovascular system.</description><identifier>ISSN: 1672-9145</identifier><identifier>EISSN: 1745-7270</identifier><identifier>DOI: 10.1093/abbs/gmq119</identifier><identifier>PMID: 21193431</identifier><language>eng</language><publisher>China</publisher><subject>Animals ; Breast Neoplasms - drug therapy ; Cell Division - drug effects ; Cell Line - drug effects ; Cyclin D1 - drug effects ; Cyclin D1 - metabolism ; Dose-Response Relationship, Drug ; Estradiol - adverse effects ; Estradiol - analogs & derivatives ; Estradiol - pharmacology ; Estrogen Antagonists - adverse effects ; Estrogen Antagonists - pharmacology ; Female ; Muscle, Smooth, Vascular - cytology ; Myocytes, Smooth Muscle - drug effects ; Myocytes, Smooth Muscle - metabolism ; Proliferating Cell Nuclear Antigen - drug effects ; Proliferating Cell Nuclear Antigen - metabolism ; Rats ; Rats, Wistar ; Receptors, Estrogen - antagonists & inhibitors ; S Phase - drug effects ; 受体拮抗剂 ; 增殖细胞核抗原 ; 心血管系统 ; 细胞增殖 ; 血管平滑肌细胞 ; 辅助治疗 ; 逆转录聚合酶链反应 ; 雌激素受体</subject><ispartof>Acta biochimica et biophysica Sinica, 2011-02, Vol.43 (2), p.118-123</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c273t-cc24cce8d2a2d40a6f442456da666eaf18cf02dfb7d82fc04e0e7a219f447d353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/90160X/90160X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21193431$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Hong</creatorcontrib><creatorcontrib>Yang, Falin</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Zeng, Xianwei</creatorcontrib><creatorcontrib>Hu, Qin</creatorcontrib><creatorcontrib>Huang, Jinghuan</creatorcontrib><creatorcontrib>Feng, Jinbo</creatorcontrib><title>Complete estrogen receptor blocker ICI182,780 promotes the proliferation of vascular smooth muscle cells</title><title>Acta biochimica et biophysica Sinica</title><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><description>ICI182,780 is used in adjuvant therapies of breast cancer. As a complete estrogen receptor （ER） blocker, ICI182,780 may antagonize the effects of estrogen on the cardiovascular system. Estrogen inhibits the proliferation of vascular smooth muscle cells （VSMCs）, which is one of the mechanisms that estrogen can exert cardioprotective effects. In the present study, to assess the effects of ICI182,780 on the proliferation of VSMCs, we cultured VSMCs isolated from rat aorta with or without the ER antagonist ICI182,780. The results of 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay, bromodeoxyuridine incorporation assay, viable cell count, immunochemicai staining for proliferating cell nuclear antigen （PCNA）, and S-phase ratio determined by flow cytometry revealed a remarkable proliferation of VSMCs after ICI182,780 treatment. ICI182,780 significantly enhanced cell growth in a dose-dependent manner （10-8-10-s M）. Furthermore, the number of PCNA-positive cells and the S-phase progression of VSMCs increased after treatment with ICI182,780. Reverse transcriptase-polymerase chain reaction （RT-PCR） and western blot analysis showed that the mRNA and protein level of cyclin D1 in VSMCs increased under the treatment of ICI182,780. These data suggested that ICI182,780 can promote the growth of VSMCs, which might produce some adverse effects on the cardiovascular system.</description><subject>Animals</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Cell Division - drug effects</subject><subject>Cell Line - drug effects</subject><subject>Cyclin D1 - drug effects</subject><subject>Cyclin D1 - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Estradiol - adverse effects</subject><subject>Estradiol - analogs & derivatives</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen Antagonists - adverse effects</subject><subject>Estrogen Antagonists - pharmacology</subject><subject>Female</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Myocytes, Smooth Muscle - drug effects</subject><subject>Myocytes, Smooth Muscle - metabolism</subject><subject>Proliferating Cell Nuclear Antigen - drug effects</subject><subject>Proliferating Cell Nuclear Antigen - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Estrogen - antagonists & inhibitors</subject><subject>S Phase - drug effects</subject><subject>受体拮抗剂</subject><subject>增殖细胞核抗原</subject><subject>心血管系统</subject><subject>细胞增殖</subject><subject>血管平滑肌细胞</subject><subject>辅助治疗</subject><subject>逆转录聚合酶链反应</subject><subject>雌激素受体</subject><issn>1672-9145</issn><issn>1745-7270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kD1PwzAQhi0EoqUwsSOLhQFC_ZU4GVHFR6VKLDBbjnNuQ524tRMk_j2JWpjuTnr06r0HoWtKHikp-FyXZZyvmz2lxQmaUinSRDJJToc9kywpqEgn6CLGL0J4llFyjiZsYLngdIo2C9_sHHSAIXbBr6HFAQzsOh9w6bzZQsDLxZLm7EHmBO-Cb3wHEXcbGA9XWwi6q32LvcXfOpre6YBj4323wU0fjQNswLl4ic6sdhGujnOGPl-ePxZvyer9dbl4WiWGSd4lxjBhDOQV06wSRGdWCCbSrNJZloG2NDeWsMqWssqZNUQAAakZLQZOVjzlM3R3yB3a7fvhKdXUcWygW_B9VLmQaSFSwQby_kCa4GMMYNUu1I0OP4oSNZpVo1l1MDvQN8fcvmyg-mf_VA7A7TFu49v1vm7XqtRma2sHikuW0pRL_gunIYIM</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>He, Hong</creator><creator>Yang, Falin</creator><creator>Wang, Xin</creator><creator>Zeng, Xianwei</creator><creator>Hu, Qin</creator><creator>Huang, Jinghuan</creator><creator>Feng, Jinbo</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110201</creationdate><title>Complete estrogen receptor blocker ICI182,780 promotes the proliferation of vascular smooth muscle cells</title><author>He, Hong ; Yang, Falin ; Wang, Xin ; Zeng, Xianwei ; Hu, Qin ; Huang, Jinghuan ; Feng, Jinbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c273t-cc24cce8d2a2d40a6f442456da666eaf18cf02dfb7d82fc04e0e7a219f447d353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Breast Neoplasms - drug therapy</topic><topic>Cell Division - drug effects</topic><topic>Cell Line - drug effects</topic><topic>Cyclin D1 - drug effects</topic><topic>Cyclin D1 - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Estradiol - adverse effects</topic><topic>Estradiol - analogs & derivatives</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen Antagonists - adverse effects</topic><topic>Estrogen Antagonists - pharmacology</topic><topic>Female</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Myocytes, Smooth Muscle - drug effects</topic><topic>Myocytes, Smooth Muscle - metabolism</topic><topic>Proliferating Cell Nuclear Antigen - drug effects</topic><topic>Proliferating Cell Nuclear Antigen - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Estrogen - antagonists & inhibitors</topic><topic>S Phase - drug effects</topic><topic>受体拮抗剂</topic><topic>增殖细胞核抗原</topic><topic>心血管系统</topic><topic>细胞增殖</topic><topic>血管平滑肌细胞</topic><topic>辅助治疗</topic><topic>逆转录聚合酶链反应</topic><topic>雌激素受体</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Hong</creatorcontrib><creatorcontrib>Yang, Falin</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Zeng, Xianwei</creatorcontrib><creatorcontrib>Hu, Qin</creatorcontrib><creatorcontrib>Huang, Jinghuan</creatorcontrib><creatorcontrib>Feng, Jinbo</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biochimica et biophysica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Hong</au><au>Yang, Falin</au><au>Wang, Xin</au><au>Zeng, Xianwei</au><au>Hu, Qin</au><au>Huang, Jinghuan</au><au>Feng, Jinbo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete estrogen receptor blocker ICI182,780 promotes the proliferation of vascular smooth muscle cells</atitle><jtitle>Acta biochimica et biophysica Sinica</jtitle><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>43</volume><issue>2</issue><spage>118</spage><epage>123</epage><pages>118-123</pages><issn>1672-9145</issn><eissn>1745-7270</eissn><abstract>ICI182,780 is used in adjuvant therapies of breast cancer. As a complete estrogen receptor （ER） blocker, ICI182,780 may antagonize the effects of estrogen on the cardiovascular system. Estrogen inhibits the proliferation of vascular smooth muscle cells （VSMCs）, which is one of the mechanisms that estrogen can exert cardioprotective effects. In the present study, to assess the effects of ICI182,780 on the proliferation of VSMCs, we cultured VSMCs isolated from rat aorta with or without the ER antagonist ICI182,780. The results of 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay, bromodeoxyuridine incorporation assay, viable cell count, immunochemicai staining for proliferating cell nuclear antigen （PCNA）, and S-phase ratio determined by flow cytometry revealed a remarkable proliferation of VSMCs after ICI182,780 treatment. ICI182,780 significantly enhanced cell growth in a dose-dependent manner （10-8-10-s M）. Furthermore, the number of PCNA-positive cells and the S-phase progression of VSMCs increased after treatment with ICI182,780. Reverse transcriptase-polymerase chain reaction （RT-PCR） and western blot analysis showed that the mRNA and protein level of cyclin D1 in VSMCs increased under the treatment of ICI182,780. These data suggested that ICI182,780 can promote the growth of VSMCs, which might produce some adverse effects on the cardiovascular system.</abstract><cop>China</cop><pmid>21193431</pmid><doi>10.1093/abbs/gmq119</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Breast Neoplasms - drug therapy Cell Division - drug effects Cell Line - drug effects Cyclin D1 - drug effects Cyclin D1 - metabolism Dose-Response Relationship, Drug Estradiol - adverse effects Estradiol - analogs & derivatives Estradiol - pharmacology Estrogen Antagonists - adverse effects Estrogen Antagonists - pharmacology Female Muscle, Smooth, Vascular - cytology Myocytes, Smooth Muscle - drug effects Myocytes, Smooth Muscle - metabolism Proliferating Cell Nuclear Antigen - drug effects Proliferating Cell Nuclear Antigen - metabolism Rats Rats, Wistar Receptors, Estrogen - antagonists & inhibitors S Phase - drug effects 受体拮抗剂 增殖细胞核抗原 心血管系统 细胞增殖 血管平滑肌细胞 辅助治疗 逆转录聚合酶链反应 雌激素受体 |
| title | Complete estrogen receptor blocker ICI182,780 promotes the proliferation of vascular smooth muscle cells |
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