Distribution of lactate dehydrogenase isozymes in subcellular fractions of rat tissues
Subcellular fractions from adult rat testis and kidney have been investigated for their lactate dehydrogenase isozymes content. A fraction from testis homogenates, showing predominance of mitochondria with dense matrix and dilated cristae, was revealed to contain most of the additional isozymes (“X”...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1970-11, Vol.141 (1), p.147-154 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | De Domenech, Estela Machado Domenech, Carlos E. Blanco, Antonio |
| description | Subcellular fractions from adult rat testis and kidney have been investigated for their lactate dehydrogenase isozymes content.
A fraction from testis homogenates, showing predominance of mitochondria with dense matrix and dilated cristae, was revealed to contain most of the additional isozymes (“X”) specific of testis and sperm. These molecular forms showed a high degree of compartmentation. A small proportion of total lactate dehydrogenase activity in kidney is associated with the microsomal fraction. That portion is entirely represented by isozyme 5, a molecular species usually not detected in whole kidney homogenates. Patterns of elution of the bound enzyme with different salt concentrations and detergents indicated that the forces involved in that association must be complex and that they possibly can operate
in vivo. |
| doi_str_mv | 10.1016/0003-9861(70)90117-7 |
| format | Article |
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A fraction from testis homogenates, showing predominance of mitochondria with dense matrix and dilated cristae, was revealed to contain most of the additional isozymes (“X”) specific of testis and sperm. These molecular forms showed a high degree of compartmentation. A small proportion of total lactate dehydrogenase activity in kidney is associated with the microsomal fraction. That portion is entirely represented by isozyme 5, a molecular species usually not detected in whole kidney homogenates. Patterns of elution of the bound enzyme with different salt concentrations and detergents indicated that the forces involved in that association must be complex and that they possibly can operate
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A fraction from testis homogenates, showing predominance of mitochondria with dense matrix and dilated cristae, was revealed to contain most of the additional isozymes (“X”) specific of testis and sperm. These molecular forms showed a high degree of compartmentation. A small proportion of total lactate dehydrogenase activity in kidney is associated with the microsomal fraction. That portion is entirely represented by isozyme 5, a molecular species usually not detected in whole kidney homogenates. Patterns of elution of the bound enzyme with different salt concentrations and detergents indicated that the forces involved in that association must be complex and that they possibly can operate
in vivo.</description><subject>Animals</subject><subject>Bile Acids and Salts - pharmacology</subject><subject>Detergents - pharmacology</subject><subject>Digitalis Glycosides - pharmacology</subject><subject>Electrophoresis</subject><subject>Isoenzymes</subject><subject>Kidney - cytology</subject><subject>Kidney - enzymology</subject><subject>L-Lactate Dehydrogenase - analysis</subject><subject>Male</subject><subject>Microsomes - drug effects</subject><subject>Microsomes - enzymology</subject><subject>Mitochondria - enzymology</subject><subject>Potassium Chloride - pharmacology</subject><subject>Rats</subject><subject>Sodium Chloride - pharmacology</subject><subject>Surface-Active Agents - pharmacology</subject><subject>Testis - cytology</subject><subject>Testis - enzymology</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhi0EKqXwD0DKhGAI2IkdJwsSKp9SJRZgtRz7DEZpAj4Hqfx6Elp1ZLrhnvc-HkKOGb1glBWXlNI8rcqCnUl6XlHGZCp3yJTRqkhpXvJdMt0i--QA8YMOEC-yCZkIXtIBnJLXG48x-LqPvmuTziWNNlFHSCy8r2zo3qDVCInH7me1BEx8m2BfG2iavtEhcWHAhySO0aBjEj1iD3hI9pxuEI42dUZe7m6f5w_p4un-cX69SE0uZEwLKV0hHa-Ftoa7XIi8MsJym1cZyILXUvK81jpjzkpRyNJoB5zb2tCaiUznM3K6nvsZuq9hb1RLj-N1uoWuR1VyKTgt5QDyNWhChxjAqc_glzqsFKNq1KlGV2p0pSRVfzrVGDvZzO_rJdhtaONv6F-t-zA8-e0hKDQeWgPWBzBR2c7_v-AXduiFsQ</recordid><startdate>197011</startdate><enddate>197011</enddate><creator>De Domenech, Estela Machado</creator><creator>Domenech, Carlos E.</creator><creator>Blanco, Antonio</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197011</creationdate><title>Distribution of lactate dehydrogenase isozymes in subcellular fractions of rat tissues</title><author>De Domenech, Estela Machado ; Domenech, Carlos E. ; Blanco, Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-677f67f4b5adc4f35539c5d4d392e764b7743baa21fd75678cafe44dbc0b152a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Animals</topic><topic>Bile Acids and Salts - pharmacology</topic><topic>Detergents - pharmacology</topic><topic>Digitalis Glycosides - pharmacology</topic><topic>Electrophoresis</topic><topic>Isoenzymes</topic><topic>Kidney - cytology</topic><topic>Kidney - enzymology</topic><topic>L-Lactate Dehydrogenase - analysis</topic><topic>Male</topic><topic>Microsomes - drug effects</topic><topic>Microsomes - enzymology</topic><topic>Mitochondria - enzymology</topic><topic>Potassium Chloride - pharmacology</topic><topic>Rats</topic><topic>Sodium Chloride - pharmacology</topic><topic>Surface-Active Agents - pharmacology</topic><topic>Testis - cytology</topic><topic>Testis - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Domenech, Estela Machado</creatorcontrib><creatorcontrib>Domenech, Carlos E.</creatorcontrib><creatorcontrib>Blanco, Antonio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Domenech, Estela Machado</au><au>Domenech, Carlos E.</au><au>Blanco, Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of lactate dehydrogenase isozymes in subcellular fractions of rat tissues</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1970-11</date><risdate>1970</risdate><volume>141</volume><issue>1</issue><spage>147</spage><epage>154</epage><pages>147-154</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>Subcellular fractions from adult rat testis and kidney have been investigated for their lactate dehydrogenase isozymes content.
A fraction from testis homogenates, showing predominance of mitochondria with dense matrix and dilated cristae, was revealed to contain most of the additional isozymes (“X”) specific of testis and sperm. These molecular forms showed a high degree of compartmentation. A small proportion of total lactate dehydrogenase activity in kidney is associated with the microsomal fraction. That portion is entirely represented by isozyme 5, a molecular species usually not detected in whole kidney homogenates. Patterns of elution of the bound enzyme with different salt concentrations and detergents indicated that the forces involved in that association must be complex and that they possibly can operate
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| ispartof | Archives of biochemistry and biophysics, 1970-11, Vol.141 (1), p.147-154 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Bile Acids and Salts - pharmacology Detergents - pharmacology Digitalis Glycosides - pharmacology Electrophoresis Isoenzymes Kidney - cytology Kidney - enzymology L-Lactate Dehydrogenase - analysis Male Microsomes - drug effects Microsomes - enzymology Mitochondria - enzymology Potassium Chloride - pharmacology Rats Sodium Chloride - pharmacology Surface-Active Agents - pharmacology Testis - cytology Testis - enzymology |
| title | Distribution of lactate dehydrogenase isozymes in subcellular fractions of rat tissues |
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