Identification of very early lymphoid precursors in bone marrow and their regulation by estrogen
Estrogen is a negative regulator of lymphopoiesis and provides an experimental tool for probing relationships between lymphocyte precursors and stem cells. We found that expression of lymphocyte-associated genes and immunoglobulin (Ig) gene rearrangement occurred before CD45R acquisition. Lymphoid-r...
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| Veröffentlicht in: | Nature immunology 2001-08, Vol.2 (8), p.718-724 |
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| creator | Kincade, Paul W Medina, Kay L Garrett, Karla P Thompson, Linda F Rossi, Maria Isabel D Payne, Kimberly J |
| description | Estrogen is a negative regulator of lymphopoiesis and provides an experimental tool for probing relationships between lymphocyte precursors and stem cells. We found that expression of lymphocyte-associated genes and immunoglobulin (Ig) gene rearrangement occurred before CD45R acquisition. Lymphoid-restricted progenitors that were Lin(-)IL-7R alpha(+)c-kit(lo)TdT(+) (lineage marker(-), interleukin receptor 7 alpha(+), c-kit(lo) and terminal deoxynucleotidyl transferase(+)) were selectively depleted in estrogen-treated mice; within a less differentiated Lin-c-kit(hi) fraction, functional precursors of B and T, but not myeloid, cells were also selectively depleted. TdT and an Ig heavy chain transgene were detected within a hormone-regulated Lin(-)c-kit(hi)Sca-1(+)CD27(+)Flk-2(+)IL-7R alpha(-) subset of this multipotential progenitor population. Identification of these extremely early lymphoid precursors should facilitate investigation of the molecular mechanisms that control lineage-fate decisions in hematopoiesis. |
| doi_str_mv | 10.1038/90659 |
| format | Article |
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We found that expression of lymphocyte-associated genes and immunoglobulin (Ig) gene rearrangement occurred before CD45R acquisition. Lymphoid-restricted progenitors that were Lin(-)IL-7R alpha(+)c-kit(lo)TdT(+) (lineage marker(-), interleukin receptor 7 alpha(+), c-kit(lo) and terminal deoxynucleotidyl transferase(+)) were selectively depleted in estrogen-treated mice; within a less differentiated Lin-c-kit(hi) fraction, functional precursors of B and T, but not myeloid, cells were also selectively depleted. TdT and an Ig heavy chain transgene were detected within a hormone-regulated Lin(-)c-kit(hi)Sca-1(+)CD27(+)Flk-2(+)IL-7R alpha(-) subset of this multipotential progenitor population. Identification of these extremely early lymphoid precursors should facilitate investigation of the molecular mechanisms that control lineage-fate decisions in hematopoiesis.</description><identifier>ISSN: 1529-2908</identifier><identifier>EISSN: 1529-2916</identifier><identifier>DOI: 10.1038/90659</identifier><identifier>PMID: 11477408</identifier><language>eng</language><publisher>United States: Nature Publishing Group</publisher><subject>Animals ; Bone marrow ; Bone Marrow Cells - cytology ; Bone Marrow Cells - physiology ; CD45R antigen ; Cell Lineage - immunology ; Estrogens ; Estrogens - physiology ; Hematopoiesis - physiology ; Lymphocytes ; Lymphocytes - cytology ; Lymphocytes - physiology ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Stem cells</subject><ispartof>Nature immunology, 2001-08, Vol.2 (8), p.718-724</ispartof><rights>COPYRIGHT 2001 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Aug 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-c4cc2d3c72edf8d473b60752b8a4b1297d269e0e2aac493cd55fd34f2d40aa0d3</citedby><cites>FETCH-LOGICAL-c489t-c4cc2d3c72edf8d473b60752b8a4b1297d269e0e2aac493cd55fd34f2d40aa0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,2728,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11477408$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kincade, Paul W</creatorcontrib><creatorcontrib>Medina, Kay L</creatorcontrib><creatorcontrib>Garrett, Karla P</creatorcontrib><creatorcontrib>Thompson, Linda F</creatorcontrib><creatorcontrib>Rossi, Maria Isabel D</creatorcontrib><creatorcontrib>Payne, Kimberly J</creatorcontrib><title>Identification of very early lymphoid precursors in bone marrow and their regulation by estrogen</title><title>Nature immunology</title><addtitle>Nat Immunol</addtitle><description>Estrogen is a negative regulator of lymphopoiesis and provides an experimental tool for probing relationships between lymphocyte precursors and stem cells. We found that expression of lymphocyte-associated genes and immunoglobulin (Ig) gene rearrangement occurred before CD45R acquisition. Lymphoid-restricted progenitors that were Lin(-)IL-7R alpha(+)c-kit(lo)TdT(+) (lineage marker(-), interleukin receptor 7 alpha(+), c-kit(lo) and terminal deoxynucleotidyl transferase(+)) were selectively depleted in estrogen-treated mice; within a less differentiated Lin-c-kit(hi) fraction, functional precursors of B and T, but not myeloid, cells were also selectively depleted. TdT and an Ig heavy chain transgene were detected within a hormone-regulated Lin(-)c-kit(hi)Sca-1(+)CD27(+)Flk-2(+)IL-7R alpha(-) subset of this multipotential progenitor population. Identification of these extremely early lymphoid precursors should facilitate investigation of the molecular mechanisms that control lineage-fate decisions in hematopoiesis.</description><subject>Animals</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - physiology</subject><subject>CD45R antigen</subject><subject>Cell Lineage - immunology</subject><subject>Estrogens</subject><subject>Estrogens - physiology</subject><subject>Hematopoiesis - physiology</subject><subject>Lymphocytes</subject><subject>Lymphocytes - cytology</subject><subject>Lymphocytes - physiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Stem cells</subject><issn>1529-2908</issn><issn>1529-2916</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0u9r1DAYB_AgDred-xckKJvsxc0kTZP05RjqDgaCP17HNHl6y2iTM2nV--_N7LFxIkohDeGTb349CJ1QckFJpd40RNTNE3REa9YsWUPF04c-UYfoOOc7QiiXgj9Dh7R0JCfqCH1dOQij77w1o48Bxw5_h7TFYFK_xf122NxG7_AmgZ1SjiljH3AbA-DBpBR_YBMcHm_BJ5xgPfVzSlsC8pjiGsJzdNCZPsPJ7r9AX969_Xx1vbz58H51dXmztFw1Y2mtZa6ykoHrlOOyagWRNWuV4S1ljXRMNECAGWN5U1lX152reMccJ8YQVy3Q6zl3k-K3qayuB58t9L0JEKesFZe8ErIWRZ79U0pK6kqI5r-QKqIYKVtdoJd_wLs4pVCOqxljJU_yuqBXM1qbHrQPXRyTsfeJ-pIqpYggXBV18RdVPgeDt-XeO1_G9yac700oZoSf49pMOevVp4_79nS2NsWcE3R6k3x5x62mRN9Xkf5dRcW92J1nagdwj2pXNgXgGQQzTgkeQPBEEVpuUFW_AFvuyb4</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>Kincade, Paul W</creator><creator>Medina, Kay L</creator><creator>Garrett, Karla P</creator><creator>Thompson, Linda F</creator><creator>Rossi, Maria Isabel D</creator><creator>Payne, Kimberly J</creator><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20010801</creationdate><title>Identification of very early lymphoid precursors in bone marrow and their regulation by estrogen</title><author>Kincade, Paul W ; Medina, Kay L ; Garrett, Karla P ; Thompson, Linda F ; Rossi, Maria Isabel D ; Payne, Kimberly J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-c4cc2d3c72edf8d473b60752b8a4b1297d269e0e2aac493cd55fd34f2d40aa0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - physiology</topic><topic>CD45R antigen</topic><topic>Cell Lineage - immunology</topic><topic>Estrogens</topic><topic>Estrogens - physiology</topic><topic>Hematopoiesis - physiology</topic><topic>Lymphocytes</topic><topic>Lymphocytes - cytology</topic><topic>Lymphocytes - physiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kincade, Paul W</creatorcontrib><creatorcontrib>Medina, Kay L</creatorcontrib><creatorcontrib>Garrett, Karla P</creatorcontrib><creatorcontrib>Thompson, Linda F</creatorcontrib><creatorcontrib>Rossi, Maria Isabel D</creatorcontrib><creatorcontrib>Payne, Kimberly J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kincade, Paul W</au><au>Medina, Kay L</au><au>Garrett, Karla P</au><au>Thompson, Linda F</au><au>Rossi, Maria Isabel D</au><au>Payne, Kimberly J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of very early lymphoid precursors in bone marrow and their regulation by estrogen</atitle><jtitle>Nature immunology</jtitle><addtitle>Nat Immunol</addtitle><date>2001-08-01</date><risdate>2001</risdate><volume>2</volume><issue>8</issue><spage>718</spage><epage>724</epage><pages>718-724</pages><issn>1529-2908</issn><eissn>1529-2916</eissn><abstract>Estrogen is a negative regulator of lymphopoiesis and provides an experimental tool for probing relationships between lymphocyte precursors and stem cells. We found that expression of lymphocyte-associated genes and immunoglobulin (Ig) gene rearrangement occurred before CD45R acquisition. Lymphoid-restricted progenitors that were Lin(-)IL-7R alpha(+)c-kit(lo)TdT(+) (lineage marker(-), interleukin receptor 7 alpha(+), c-kit(lo) and terminal deoxynucleotidyl transferase(+)) were selectively depleted in estrogen-treated mice; within a less differentiated Lin-c-kit(hi) fraction, functional precursors of B and T, but not myeloid, cells were also selectively depleted. TdT and an Ig heavy chain transgene were detected within a hormone-regulated Lin(-)c-kit(hi)Sca-1(+)CD27(+)Flk-2(+)IL-7R alpha(-) subset of this multipotential progenitor population. Identification of these extremely early lymphoid precursors should facilitate investigation of the molecular mechanisms that control lineage-fate decisions in hematopoiesis.</abstract><cop>United States</cop><pub>Nature Publishing Group</pub><pmid>11477408</pmid><doi>10.1038/90659</doi><tpages>7</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Nature; Alma/SFX Local Collection |
| subjects | Animals Bone marrow Bone Marrow Cells - cytology Bone Marrow Cells - physiology CD45R antigen Cell Lineage - immunology Estrogens Estrogens - physiology Hematopoiesis - physiology Lymphocytes Lymphocytes - cytology Lymphocytes - physiology Mice Mice, Inbred BALB C Mice, Inbred C57BL Stem cells |
| title | Identification of very early lymphoid precursors in bone marrow and their regulation by estrogen |
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