Rapid hydrophilic interaction chromatography determination of lysine in pharmaceutical preparations with fluorescence detection after postcolumn derivatization with o-phtaldialdehyde
A rapid procedure for the determination of lysine based on hydrophilic interaction chromatography (HILIC) separation of arginine and lysine with fluorescence detection has been developed. The separation conditions and parameters of lysine postcolumn derivatization with o-phtaldialdehyde (OPA)/2-merc...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2011-04, Vol.54 (5), p.972-978 |
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| container_issue | 5 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Douša, Michal Břicháč, Jiří Gibala, Petr Lehnert, Petr |
| description | A rapid procedure for the determination of lysine based on hydrophilic interaction chromatography (HILIC) separation of arginine and lysine with fluorescence detection has been developed. The separation conditions and parameters of lysine postcolumn derivatization with
o-phtaldialdehyde (OPA)/2-mercaptoethanol were studied. The various HILIC columns were employed using isocratic elution. Fluorescence detection was performed at excitation and emission wavelength of 345
nm and 450
nm, respectively. An advantage of the reported method is a simple sample pre-treatment and a quick and very sensitive HPLC method. The developed method was successfully applied for analysis of commercial samples of Ibalgin Fast tablets (Zentiva, Czech Republic). |
| doi_str_mv | 10.1016/j.jpba.2010.11.026 |
| format | Article |
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o-phtaldialdehyde (OPA)/2-mercaptoethanol were studied. The various HILIC columns were employed using isocratic elution. Fluorescence detection was performed at excitation and emission wavelength of 345
nm and 450
nm, respectively. An advantage of the reported method is a simple sample pre-treatment and a quick and very sensitive HPLC method. The developed method was successfully applied for analysis of commercial samples of Ibalgin Fast tablets (Zentiva, Czech Republic).</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2010.11.026</identifier><identifier>PMID: 21163603</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Arginine ; Arginine - analysis ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; derivatization ; drugs ; fluorescence ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; high performance liquid chromatography ; HILIC ; hydrophilic interaction chromatography ; Hydrophobic and Hydrophilic Interactions ; Indicators and Reagents ; Limit of Detection ; Lysine ; Lysine - isolation & purification ; Medical sciences ; Mercaptoethanol - chemistry ; o-Phthalaldehyde - chemistry ; Pharmaceutical Preparations - analysis ; Pharmacology. Drug treatments ; Postcolumn derivatization ; pretreatment ; Reference Standards ; Reproducibility of Results ; Spectrometry, Fluorescence ; Time Factors ; wavelengths</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2011-04, Vol.54 (5), p.972-978</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-b87c38ab8cd20bcb1f7ad99fbce8c27552a0192be905f4a22a21c9dcd71bce8c3</citedby><cites>FETCH-LOGICAL-c409t-b87c38ab8cd20bcb1f7ad99fbce8c27552a0192be905f4a22a21c9dcd71bce8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708510006692$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23871092$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21163603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Douša, Michal</creatorcontrib><creatorcontrib>Břicháč, Jiří</creatorcontrib><creatorcontrib>Gibala, Petr</creatorcontrib><creatorcontrib>Lehnert, Petr</creatorcontrib><title>Rapid hydrophilic interaction chromatography determination of lysine in pharmaceutical preparations with fluorescence detection after postcolumn derivatization with o-phtaldialdehyde</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A rapid procedure for the determination of lysine based on hydrophilic interaction chromatography (HILIC) separation of arginine and lysine with fluorescence detection has been developed. The separation conditions and parameters of lysine postcolumn derivatization with
o-phtaldialdehyde (OPA)/2-mercaptoethanol were studied. The various HILIC columns were employed using isocratic elution. Fluorescence detection was performed at excitation and emission wavelength of 345
nm and 450
nm, respectively. An advantage of the reported method is a simple sample pre-treatment and a quick and very sensitive HPLC method. The developed method was successfully applied for analysis of commercial samples of Ibalgin Fast tablets (Zentiva, Czech Republic).</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Arginine</subject><subject>Arginine - analysis</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>derivatization</subject><subject>drugs</subject><subject>fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>high performance liquid chromatography</subject><subject>HILIC</subject><subject>hydrophilic interaction chromatography</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Indicators and Reagents</subject><subject>Limit of Detection</subject><subject>Lysine</subject><subject>Lysine - isolation & purification</subject><subject>Medical sciences</subject><subject>Mercaptoethanol - chemistry</subject><subject>o-Phthalaldehyde - chemistry</subject><subject>Pharmaceutical Preparations - analysis</subject><subject>Pharmacology. Drug treatments</subject><subject>Postcolumn derivatization</subject><subject>pretreatment</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Spectrometry, Fluorescence</subject><subject>Time Factors</subject><subject>wavelengths</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuO1DAQRSMEYpqBH2AB3iBWacrOw4nEBo14SSMhASOxsyp-TNxKYmMng5oP4_twOg3sWFiWXOfWLdfNsqcU9hRo_eqwP_gO9wzWB7oHVt_LdrThRc7q8tv9bAe8oDmHprrIHsV4AICKtuXD7IJRWhc1FLvs12f0VpH-qILzvR2sJHaadUA5WzcR2Qc34uxuA_r-SJROpdFOeCo6Q4ZjtJNOEuJ7DCNKvcxW4kB80B7DiYvkh517YobFBR2lnqQ-Ndoc0KSWxLs4Szcs45RKwd4l4c_N5KR1ue9nHJRNR6dZ9ePsgcEh6ifn-zK7eff269WH_PrT-49Xb65zWUI7513DZdFg10jFoJMdNRxV25pO6kYyXlUMgbas0y1UpkTGkFHZKqk4PSHFZfZy6-uD-77oOIvRpi8MA07aLVE0JS8LVkGdSLaRMrgYgzbCBztiOAoKYo1LHMQal1jjEpSKFFcSPTu3X7pRq7-SP_kk4MUZwJjWagJO0sZ_XNFwCi1L3PONM-gE3obE3HxJThUAZVDy1er1Rui0rjurg4jSrlkoG1IUQjn7v0l_AzN1xMU</recordid><startdate>20110405</startdate><enddate>20110405</enddate><creator>Douša, Michal</creator><creator>Břicháč, Jiří</creator><creator>Gibala, Petr</creator><creator>Lehnert, Petr</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110405</creationdate><title>Rapid hydrophilic interaction chromatography determination of lysine in pharmaceutical preparations with fluorescence detection after postcolumn derivatization with o-phtaldialdehyde</title><author>Douša, Michal ; Břicháč, Jiří ; Gibala, Petr ; Lehnert, Petr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-b87c38ab8cd20bcb1f7ad99fbce8c27552a0192be905f4a22a21c9dcd71bce8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Arginine</topic><topic>Arginine - analysis</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>derivatization</topic><topic>drugs</topic><topic>fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>high performance liquid chromatography</topic><topic>HILIC</topic><topic>hydrophilic interaction chromatography</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Indicators and Reagents</topic><topic>Limit of Detection</topic><topic>Lysine</topic><topic>Lysine - isolation & purification</topic><topic>Medical sciences</topic><topic>Mercaptoethanol - chemistry</topic><topic>o-Phthalaldehyde - chemistry</topic><topic>Pharmaceutical Preparations - analysis</topic><topic>Pharmacology. Drug treatments</topic><topic>Postcolumn derivatization</topic><topic>pretreatment</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Spectrometry, Fluorescence</topic><topic>Time Factors</topic><topic>wavelengths</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Douša, Michal</creatorcontrib><creatorcontrib>Břicháč, Jiří</creatorcontrib><creatorcontrib>Gibala, Petr</creatorcontrib><creatorcontrib>Lehnert, Petr</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Douša, Michal</au><au>Břicháč, Jiří</au><au>Gibala, Petr</au><au>Lehnert, Petr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid hydrophilic interaction chromatography determination of lysine in pharmaceutical preparations with fluorescence detection after postcolumn derivatization with o-phtaldialdehyde</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2011-04-05</date><risdate>2011</risdate><volume>54</volume><issue>5</issue><spage>972</spage><epage>978</epage><pages>972-978</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A rapid procedure for the determination of lysine based on hydrophilic interaction chromatography (HILIC) separation of arginine and lysine with fluorescence detection has been developed. The separation conditions and parameters of lysine postcolumn derivatization with
o-phtaldialdehyde (OPA)/2-mercaptoethanol were studied. The various HILIC columns were employed using isocratic elution. Fluorescence detection was performed at excitation and emission wavelength of 345
nm and 450
nm, respectively. An advantage of the reported method is a simple sample pre-treatment and a quick and very sensitive HPLC method. The developed method was successfully applied for analysis of commercial samples of Ibalgin Fast tablets (Zentiva, Czech Republic).</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21163603</pmid><doi>10.1016/j.jpba.2010.11.026</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analysis Analytical, structural and metabolic biochemistry Arginine Arginine - analysis Biological and medical sciences Chromatography, High Pressure Liquid - methods derivatization drugs fluorescence Fundamental and applied biological sciences. Psychology General pharmacology high performance liquid chromatography HILIC hydrophilic interaction chromatography Hydrophobic and Hydrophilic Interactions Indicators and Reagents Limit of Detection Lysine Lysine - isolation & purification Medical sciences Mercaptoethanol - chemistry o-Phthalaldehyde - chemistry Pharmaceutical Preparations - analysis Pharmacology. Drug treatments Postcolumn derivatization pretreatment Reference Standards Reproducibility of Results Spectrometry, Fluorescence Time Factors wavelengths |
| title | Rapid hydrophilic interaction chromatography determination of lysine in pharmaceutical preparations with fluorescence detection after postcolumn derivatization with o-phtaldialdehyde |
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