Tumor lipids: Structural analyses of the phospholipids
The distribution of fatty acids esterified at the 1- and 2-positions of phosphatidyl serine (PS), phosphatidyl inositol (PI), and diphosphatidyl glycerol (DPG), and the fatty acid composition of sphingomyelins and ceramides isolated from Ehrlich ascites cells were determined. Diglycerides derived fr...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1970-11, Vol.141 (1), p.183-189 |
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| creator | Wood, Randall Harlow, R.D. |
| description | The distribution of fatty acids esterified at the 1- and 2-positions of phosphatidyl serine (PS), phosphatidyl inositol (PI), and diphosphatidyl glycerol (DPG), and the fatty acid composition of sphingomyelins and ceramides isolated from Ehrlich ascites cells were determined. Diglycerides derived from PS, PI, and DPG by phospholipase C hydrolysis were also analyzed intact as acetate derivatives.
Alkyl and alk-1-enyl glyceryl ethers were absent from PS, PI, and DPG, or present in only trace amounts. Predominately stearic acid was esterified at the 1-position of PS and PI. Stearic, 18:1, and 18:2 acids accounted for 34, 25, and 25% of the acids esterified at the 2-position of PS, whereas two-thirds of the 2-position acids of PI was 20:4. Both the 1- and 2-positions of DPG were dominated by 18:2 acid. More than 75% of the DPG was resistant to phospholipase C hydrolysis. The distribution of diglyceride species was different for each phosphatide.
Sphingomyelin and ceramide fatty acids obtained from this neoplasm contained 9% of a C
24 dienoic acid, which is absent from sphingomyelin of normal tissue, or present in only trace amounts. The acid was identified as Δ
15, Δ
18-tetracosadienoic acid. |
| doi_str_mv | 10.1016/0003-9861(70)90121-9 |
| format | Article |
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Alkyl and alk-1-enyl glyceryl ethers were absent from PS, PI, and DPG, or present in only trace amounts. Predominately stearic acid was esterified at the 1-position of PS and PI. Stearic, 18:1, and 18:2 acids accounted for 34, 25, and 25% of the acids esterified at the 2-position of PS, whereas two-thirds of the 2-position acids of PI was 20:4. Both the 1- and 2-positions of DPG were dominated by 18:2 acid. More than 75% of the DPG was resistant to phospholipase C hydrolysis. The distribution of diglyceride species was different for each phosphatide.
Sphingomyelin and ceramide fatty acids obtained from this neoplasm contained 9% of a C
24 dienoic acid, which is absent from sphingomyelin of normal tissue, or present in only trace amounts. The acid was identified as Δ
15, Δ
18-tetracosadienoic acid.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(70)90121-9</identifier><identifier>PMID: 4320351</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Alcohols - analysis ; Animals ; Carcinoma, Ehrlich Tumor ; Chromatography, Gas ; Fatty Acids - analysis ; Glycerides - analysis ; Glycols - analysis ; Mice ; Phosphatidylethanolamines - analysis ; Phosphatidylinositols - analysis ; Phospholipases ; Phospholipids - analysis ; Sphingomyelins - analysis ; Stearic Acids - analysis</subject><ispartof>Archives of biochemistry and biophysics, 1970-11, Vol.141 (1), p.183-189</ispartof><rights>1970</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-90a6cf8b5ba15741a31a3e44f42f99c699de145bfc4e4966ca394801975e49943</citedby><cites>FETCH-LOGICAL-c357t-90a6cf8b5ba15741a31a3e44f42f99c699de145bfc4e4966ca394801975e49943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(70)90121-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4320351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wood, Randall</creatorcontrib><creatorcontrib>Harlow, R.D.</creatorcontrib><title>Tumor lipids: Structural analyses of the phospholipids</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>The distribution of fatty acids esterified at the 1- and 2-positions of phosphatidyl serine (PS), phosphatidyl inositol (PI), and diphosphatidyl glycerol (DPG), and the fatty acid composition of sphingomyelins and ceramides isolated from Ehrlich ascites cells were determined. Diglycerides derived from PS, PI, and DPG by phospholipase C hydrolysis were also analyzed intact as acetate derivatives.
Alkyl and alk-1-enyl glyceryl ethers were absent from PS, PI, and DPG, or present in only trace amounts. Predominately stearic acid was esterified at the 1-position of PS and PI. Stearic, 18:1, and 18:2 acids accounted for 34, 25, and 25% of the acids esterified at the 2-position of PS, whereas two-thirds of the 2-position acids of PI was 20:4. Both the 1- and 2-positions of DPG were dominated by 18:2 acid. More than 75% of the DPG was resistant to phospholipase C hydrolysis. The distribution of diglyceride species was different for each phosphatide.
Sphingomyelin and ceramide fatty acids obtained from this neoplasm contained 9% of a C
24 dienoic acid, which is absent from sphingomyelin of normal tissue, or present in only trace amounts. The acid was identified as Δ
15, Δ
18-tetracosadienoic acid.</description><subject>Amino Alcohols - analysis</subject><subject>Animals</subject><subject>Carcinoma, Ehrlich Tumor</subject><subject>Chromatography, Gas</subject><subject>Fatty Acids - analysis</subject><subject>Glycerides - analysis</subject><subject>Glycols - analysis</subject><subject>Mice</subject><subject>Phosphatidylethanolamines - analysis</subject><subject>Phosphatidylinositols - analysis</subject><subject>Phospholipases</subject><subject>Phospholipids - analysis</subject><subject>Sphingomyelins - analysis</subject><subject>Stearic Acids - analysis</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1Lw0AQhhdRaq3-A4WcRA_Rmexmk_UgSPELCh6s52WzmdCVpKm7idB_b2qKR2GGYZh33mEexs4RbhBQ3gIAj1Uu8SqDawWYYKwO2BRByRh4Lg7Z9E9yzE5C-ARAFDKZsIngCfAUp0wu-6b1Ue02rgx30Xvne9v13tSRWZt6GyhEbRV1K4o2qzYMOSpP2VFl6kBn-zpjH0-Py_lLvHh7fp0_LGLL06yLFRhpq7xIC4NpJtDwIUiISiSVUlYqVRKKtKisIKGktIYrkQOqLB16JfiMXY6-G99-9RQ63bhgqa7Nmto-6FxkXOXAB6EYhda3IXiq9Ma7xvitRtA7XHrHQu9Y6Az0Ly6thrWLvX9fNFT-Le35DPP7cU7Dk9-OvA7W0dpS6TzZTpet-__AD-Z-eCI</recordid><startdate>197011</startdate><enddate>197011</enddate><creator>Wood, Randall</creator><creator>Harlow, R.D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197011</creationdate><title>Tumor lipids: Structural analyses of the phospholipids</title><author>Wood, Randall ; Harlow, R.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-90a6cf8b5ba15741a31a3e44f42f99c699de145bfc4e4966ca394801975e49943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Amino Alcohols - analysis</topic><topic>Animals</topic><topic>Carcinoma, Ehrlich Tumor</topic><topic>Chromatography, Gas</topic><topic>Fatty Acids - analysis</topic><topic>Glycerides - analysis</topic><topic>Glycols - analysis</topic><topic>Mice</topic><topic>Phosphatidylethanolamines - analysis</topic><topic>Phosphatidylinositols - analysis</topic><topic>Phospholipases</topic><topic>Phospholipids - analysis</topic><topic>Sphingomyelins - analysis</topic><topic>Stearic Acids - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wood, Randall</creatorcontrib><creatorcontrib>Harlow, R.D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wood, Randall</au><au>Harlow, R.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tumor lipids: Structural analyses of the phospholipids</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1970-11</date><risdate>1970</risdate><volume>141</volume><issue>1</issue><spage>183</spage><epage>189</epage><pages>183-189</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>The distribution of fatty acids esterified at the 1- and 2-positions of phosphatidyl serine (PS), phosphatidyl inositol (PI), and diphosphatidyl glycerol (DPG), and the fatty acid composition of sphingomyelins and ceramides isolated from Ehrlich ascites cells were determined. Diglycerides derived from PS, PI, and DPG by phospholipase C hydrolysis were also analyzed intact as acetate derivatives.
Alkyl and alk-1-enyl glyceryl ethers were absent from PS, PI, and DPG, or present in only trace amounts. Predominately stearic acid was esterified at the 1-position of PS and PI. Stearic, 18:1, and 18:2 acids accounted for 34, 25, and 25% of the acids esterified at the 2-position of PS, whereas two-thirds of the 2-position acids of PI was 20:4. Both the 1- and 2-positions of DPG were dominated by 18:2 acid. More than 75% of the DPG was resistant to phospholipase C hydrolysis. The distribution of diglyceride species was different for each phosphatide.
Sphingomyelin and ceramide fatty acids obtained from this neoplasm contained 9% of a C
24 dienoic acid, which is absent from sphingomyelin of normal tissue, or present in only trace amounts. The acid was identified as Δ
15, Δ
18-tetracosadienoic acid.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4320351</pmid><doi>10.1016/0003-9861(70)90121-9</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1970-11, Vol.141 (1), p.183-189 |
| issn | 0003-9861 1096-0384 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Alcohols - analysis Animals Carcinoma, Ehrlich Tumor Chromatography, Gas Fatty Acids - analysis Glycerides - analysis Glycols - analysis Mice Phosphatidylethanolamines - analysis Phosphatidylinositols - analysis Phospholipases Phospholipids - analysis Sphingomyelins - analysis Stearic Acids - analysis |
| title | Tumor lipids: Structural analyses of the phospholipids |
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