Ionic strength-dependent denaturation of Thermomyces lanuginosus lipase induced by SDS

▸ Thermomyces lanuginosus lipase (TlL) is denatured by SDS at low ionic strength. ▸ The SDS-denatured state contains secondary but no native, tertiary structure. ▸ TlL is not denatured by SDS at high ionic strength. ▸ Denaturation is correlated with the effect of ionic strength on micelle formation....

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Veröffentlicht in:	Archives of biochemistry and biophysics 2011-02, Vol.506 (1), p.92-98
Hauptverfasser:	Fano, Mathias, van de Weert, Marco, Moeller, Eva Horn, Kruse, Nanna Aaby, Frokjaer, Sven
Format:	Artikel
Sprache:	eng
Schlagworte:	Ascomycota - enzymology Catalytic Domain Circular Dichroism Critical micelle concentration Denaturation fluorescence emission spectroscopy Fluorescence Polarization fluorescence spectroscopy Fungal Proteins - chemistry Fungal Proteins - metabolism Ionic strength Lipase - chemistry Lipase - metabolism Micelles Monomers Osmolar Concentration pH effects Phosphate Protein Denaturation - drug effects Protein structure Protein Structure, Tertiary proteins Protein–surfactant interactions Salts Sodium chloride Sodium dodecyl sulfate Sodium Dodecyl Sulfate - pharmacology Sodium lauryl sulfate Spectrometry, Fluorescence Surface-Active Agents - pharmacology Surfactants Tertiary structure Thermomyces lanuginosus Thermomyces lanuginosus lipase Triacylglycerol lipase Triglycerides
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