Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake
► In this study we succeeded in developing an octaarginine modified liposome equipped with PC-SOD (R8-LP (PC-SOD)). ► R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. ► R8-LP (PC-SOD) has the ability to deliver PC-SOD to the cytosol efficiently. ► R8-LP (PC-SOD) was scavenged super...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2011-01, Vol.404 (3), p.796-801 |
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| creator | Furukawa, Ryo Yamada, Yuma Takenaga, Mitsuko Igarashi, Rie Harashima, Hideyoshi |
| description | ► In this study we succeeded in developing an octaarginine modified liposome equipped with PC-SOD (R8-LP (PC-SOD)). ► R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. ► R8-LP (PC-SOD) has the ability to deliver PC-SOD to the cytosol efficiently. ► R8-LP (PC-SOD) was scavenged superoxide anions (O
2
-) very efficiently. ► The efficient cytosolic delivery by R8-LP enhances the anti-oxidant effect of PC-SOD.
The anti-oxidant enzyme superoxide dismutase (SOD) has the potential for use as a therapeutic agent in the treatment of various diseases caused by reactive oxygen species. However, achieving this would be difficult without a suitable delivery system for SOD. We previously reported that PC-SOD, in which four molecules of a phosphatidylcholine (PC) derivative were covalently bound to each dimer of recombinant human CuZnSOD, was a high affinity for the cell membrane
[14]. Here, we show that an octaarginine (R8) modified liposome equipped with PC-SOD (R8-LP (PC-SOD)) enhances its anti-oxidant effect. High-density R8-modified liposomes can stimulate macropinocytosis and are taken up efficiently by cells as demonstrated in a previous study
[21]. Flow cytometry analyses showed that R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. Moreover, R8-LP (PC-SOD) liposomes were found to scavenge superoxide anions (O
2
−) very efficiently. These results suggest that the efficient cytosolic delivery of PC-SOD by R8-modified liposomes would enhance the anti-oxidant effects of PC-SOD. |
| doi_str_mv | 10.1016/j.bbrc.2010.12.062 |
| format | Article |
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2
-) very efficiently. ► The efficient cytosolic delivery by R8-LP enhances the anti-oxidant effect of PC-SOD.
The anti-oxidant enzyme superoxide dismutase (SOD) has the potential for use as a therapeutic agent in the treatment of various diseases caused by reactive oxygen species. However, achieving this would be difficult without a suitable delivery system for SOD. We previously reported that PC-SOD, in which four molecules of a phosphatidylcholine (PC) derivative were covalently bound to each dimer of recombinant human CuZnSOD, was a high affinity for the cell membrane
[14]. Here, we show that an octaarginine (R8) modified liposome equipped with PC-SOD (R8-LP (PC-SOD)) enhances its anti-oxidant effect. High-density R8-modified liposomes can stimulate macropinocytosis and are taken up efficiently by cells as demonstrated in a previous study
[21]. Flow cytometry analyses showed that R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. Moreover, R8-LP (PC-SOD) liposomes were found to scavenge superoxide anions (O
2
−) very efficiently. These results suggest that the efficient cytosolic delivery of PC-SOD by R8-modified liposomes would enhance the anti-oxidant effects of PC-SOD.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2010.12.062</identifier><identifier>PMID: 21168389</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Anti-oxidant therapy ; Antioxidants - administration & dosage ; Antioxidants - chemistry ; Antioxidants - metabolism ; Flow Cytometry ; HeLa Cells ; Humans ; Lecithinized superoxide dismutase (PC-SOD) ; Liposomes ; Mitochondria ; Octaarginine-modified liposomes ; Oligopeptides - chemistry ; Phosphatidylcholines - administration & dosage ; Phosphatidylcholines - chemistry ; Phosphatidylcholines - metabolism ; Reactive oxygen species (ROS) ; Superoxide Dismutase - administration & dosage ; Superoxide Dismutase - chemistry ; Superoxide Dismutase - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2011-01, Vol.404 (3), p.796-801</ispartof><rights>2010 Elsevier Inc.</rights><rights>Copyright © 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-a116bdde2d75ccfc8da0156ee206e98da4b37064f08e157813a885f6719602c73</citedby><cites>FETCH-LOGICAL-c509t-a116bdde2d75ccfc8da0156ee206e98da4b37064f08e157813a885f6719602c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2010.12.062$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21168389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Furukawa, Ryo</creatorcontrib><creatorcontrib>Yamada, Yuma</creatorcontrib><creatorcontrib>Takenaga, Mitsuko</creatorcontrib><creatorcontrib>Igarashi, Rie</creatorcontrib><creatorcontrib>Harashima, Hideyoshi</creatorcontrib><title>Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>► In this study we succeeded in developing an octaarginine modified liposome equipped with PC-SOD (R8-LP (PC-SOD)). ► R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. ► R8-LP (PC-SOD) has the ability to deliver PC-SOD to the cytosol efficiently. ► R8-LP (PC-SOD) was scavenged superoxide anions (O
2
-) very efficiently. ► The efficient cytosolic delivery by R8-LP enhances the anti-oxidant effect of PC-SOD.
The anti-oxidant enzyme superoxide dismutase (SOD) has the potential for use as a therapeutic agent in the treatment of various diseases caused by reactive oxygen species. However, achieving this would be difficult without a suitable delivery system for SOD. We previously reported that PC-SOD, in which four molecules of a phosphatidylcholine (PC) derivative were covalently bound to each dimer of recombinant human CuZnSOD, was a high affinity for the cell membrane
[14]. Here, we show that an octaarginine (R8) modified liposome equipped with PC-SOD (R8-LP (PC-SOD)) enhances its anti-oxidant effect. High-density R8-modified liposomes can stimulate macropinocytosis and are taken up efficiently by cells as demonstrated in a previous study
[21]. Flow cytometry analyses showed that R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. Moreover, R8-LP (PC-SOD) liposomes were found to scavenge superoxide anions (O
2
−) very efficiently. These results suggest that the efficient cytosolic delivery of PC-SOD by R8-modified liposomes would enhance the anti-oxidant effects of PC-SOD.</description><subject>Anti-oxidant therapy</subject><subject>Antioxidants - administration & dosage</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - metabolism</subject><subject>Flow Cytometry</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Lecithinized superoxide dismutase (PC-SOD)</subject><subject>Liposomes</subject><subject>Mitochondria</subject><subject>Octaarginine-modified liposomes</subject><subject>Oligopeptides - chemistry</subject><subject>Phosphatidylcholines - administration & dosage</subject><subject>Phosphatidylcholines - chemistry</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Reactive oxygen species (ROS)</subject><subject>Superoxide Dismutase - administration & dosage</subject><subject>Superoxide Dismutase - chemistry</subject><subject>Superoxide Dismutase - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtu1DAUhi1ERaeFF2CBvGOV4diTOI7EBlXlIo3UDZXYWY593PGQxMF2KsoD9LnraApLVr7o-3-d8xHylsGWARMfjtu-j2bLYf3gWxD8Bdkw6KDiDOqXZAMAouId-3FOLlI6AjBWi-4VOeeMCbmT3YY83pisdbzzk5-wGoP1zqOlg59DCiMmitNBTwZpPiDVU_ZV-O1tuVB0Dk2mwdE9Gp8PpeFPSaZlxrgySK1P45J1Qto_UD-ZiDr56Y76nKjBYVgGHekyZ_0TX5Mzp4eEb57PS3L7-fr71ddqf_Pl29WnfWUa6HKly9y9tcht2xjjjLQaWCMQOQjsyqvudy2I2oFE1rSS7bSUjRMt6wRw0-4uyftT7xzDrwVTVqNP6yx6wrAkJeuWt7Kr60LyE2liSCmiU3P0o44PioFa9aujWvWrVb9iXBX9JfTuuX7pR7T_In99F-DjCcCy5L3HqJLxWPxaH4tNZYP_X_8TkJWZjg</recordid><startdate>20110121</startdate><enddate>20110121</enddate><creator>Furukawa, Ryo</creator><creator>Yamada, Yuma</creator><creator>Takenaga, Mitsuko</creator><creator>Igarashi, Rie</creator><creator>Harashima, Hideyoshi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110121</creationdate><title>Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake</title><author>Furukawa, Ryo ; Yamada, Yuma ; Takenaga, Mitsuko ; Igarashi, Rie ; Harashima, Hideyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-a116bdde2d75ccfc8da0156ee206e98da4b37064f08e157813a885f6719602c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Anti-oxidant therapy</topic><topic>Antioxidants - administration & dosage</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - metabolism</topic><topic>Flow Cytometry</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Lecithinized superoxide dismutase (PC-SOD)</topic><topic>Liposomes</topic><topic>Mitochondria</topic><topic>Octaarginine-modified liposomes</topic><topic>Oligopeptides - chemistry</topic><topic>Phosphatidylcholines - administration & dosage</topic><topic>Phosphatidylcholines - chemistry</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Reactive oxygen species (ROS)</topic><topic>Superoxide Dismutase - administration & dosage</topic><topic>Superoxide Dismutase - chemistry</topic><topic>Superoxide Dismutase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Furukawa, Ryo</creatorcontrib><creatorcontrib>Yamada, Yuma</creatorcontrib><creatorcontrib>Takenaga, Mitsuko</creatorcontrib><creatorcontrib>Igarashi, Rie</creatorcontrib><creatorcontrib>Harashima, Hideyoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Furukawa, Ryo</au><au>Yamada, Yuma</au><au>Takenaga, Mitsuko</au><au>Igarashi, Rie</au><au>Harashima, Hideyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2011-01-21</date><risdate>2011</risdate><volume>404</volume><issue>3</issue><spage>796</spage><epage>801</epage><pages>796-801</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>► In this study we succeeded in developing an octaarginine modified liposome equipped with PC-SOD (R8-LP (PC-SOD)). ► R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. ► R8-LP (PC-SOD) has the ability to deliver PC-SOD to the cytosol efficiently. ► R8-LP (PC-SOD) was scavenged superoxide anions (O
2
-) very efficiently. ► The efficient cytosolic delivery by R8-LP enhances the anti-oxidant effect of PC-SOD.
The anti-oxidant enzyme superoxide dismutase (SOD) has the potential for use as a therapeutic agent in the treatment of various diseases caused by reactive oxygen species. However, achieving this would be difficult without a suitable delivery system for SOD. We previously reported that PC-SOD, in which four molecules of a phosphatidylcholine (PC) derivative were covalently bound to each dimer of recombinant human CuZnSOD, was a high affinity for the cell membrane
[14]. Here, we show that an octaarginine (R8) modified liposome equipped with PC-SOD (R8-LP (PC-SOD)) enhances its anti-oxidant effect. High-density R8-modified liposomes can stimulate macropinocytosis and are taken up efficiently by cells as demonstrated in a previous study
[21]. Flow cytometry analyses showed that R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. Moreover, R8-LP (PC-SOD) liposomes were found to scavenge superoxide anions (O
2
−) very efficiently. These results suggest that the efficient cytosolic delivery of PC-SOD by R8-modified liposomes would enhance the anti-oxidant effects of PC-SOD.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21168389</pmid><doi>10.1016/j.bbrc.2010.12.062</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-oxidant therapy Antioxidants - administration & dosage Antioxidants - chemistry Antioxidants - metabolism Flow Cytometry HeLa Cells Humans Lecithinized superoxide dismutase (PC-SOD) Liposomes Mitochondria Octaarginine-modified liposomes Oligopeptides - chemistry Phosphatidylcholines - administration & dosage Phosphatidylcholines - chemistry Phosphatidylcholines - metabolism Reactive oxygen species (ROS) Superoxide Dismutase - administration & dosage Superoxide Dismutase - chemistry Superoxide Dismutase - metabolism |
| title | Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake |
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