Fine structural changes in the nucleus induced by adenosine
Cells of the ME-180 cell line, when treated with a nutrient medium that contains adenosine in a 2 m M concentration, show profound fine structural changes of nucleoli. The fibrillar and granular components become separated, and new structural elements appear that are not observed in nucleoli of untr...
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| Veröffentlicht in: | Journal of ultrastructure research 1970-08, Vol.32 (3), p.212-225 |
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| creator | Recher, Louis |
| description | Cells of the ME-180 cell line, when treated with a nutrient medium that contains adenosine in a 2 m
M concentration, show profound fine structural changes of nucleoli. The fibrillar and granular components become separated, and new structural elements appear that are not observed in nucleoli of untreated cells. Most prominent among those structural elements are electron dense nodules of 100–170 m
μ size that appear to be composed primarily of proteins and that resist lead citrate staining unless they are stained first with uranyl acetate. Nodules of identical appearance may occur also outside nucleoli. These appear to be associated with the dispersed chromatin.
The mechanism by which adenosine exerts its effect on nucleoli is unknown. A meaningful interpretation is impossible at the present time, since little is known of its biochemical effects. |
| doi_str_mv | 10.1016/S0022-5320(70)80002-8 |
| format | Article |
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M concentration, show profound fine structural changes of nucleoli. The fibrillar and granular components become separated, and new structural elements appear that are not observed in nucleoli of untreated cells. Most prominent among those structural elements are electron dense nodules of 100–170 m
μ size that appear to be composed primarily of proteins and that resist lead citrate staining unless they are stained first with uranyl acetate. Nodules of identical appearance may occur also outside nucleoli. These appear to be associated with the dispersed chromatin.
The mechanism by which adenosine exerts its effect on nucleoli is unknown. A meaningful interpretation is impossible at the present time, since little is known of its biochemical effects.</description><identifier>ISSN: 0022-5320</identifier><identifier>EISSN: 1878-2345</identifier><identifier>DOI: 10.1016/S0022-5320(70)80002-8</identifier><identifier>PMID: 4917989</identifier><language>eng</language><publisher>United States: Elsevier B.V</publisher><subject>Carcinoma - pathology ; Cell Line - drug effects ; Cell Nucleolus ; Culture Techniques ; Female ; Humans ; Microscopy, Electron ; Nucleosides - pharmacology ; Pepsin A - pharmacology ; Ribonucleases - pharmacology ; Trypsin - pharmacology ; Uterine Cervical Neoplasms - pathology</subject><ispartof>Journal of ultrastructure research, 1970-08, Vol.32 (3), p.212-225</ispartof><rights>1970 Academic Press, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-860ebcfdf8f313427f86fee6e7e77a82600542c60592587598f7d98a70e6eddf3</citedby><cites>FETCH-LOGICAL-c360t-860ebcfdf8f313427f86fee6e7e77a82600542c60592587598f7d98a70e6eddf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4917989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Recher, Louis</creatorcontrib><title>Fine structural changes in the nucleus induced by adenosine</title><title>Journal of ultrastructure research</title><addtitle>J Ultrastruct Res</addtitle><description>Cells of the ME-180 cell line, when treated with a nutrient medium that contains adenosine in a 2 m
M concentration, show profound fine structural changes of nucleoli. The fibrillar and granular components become separated, and new structural elements appear that are not observed in nucleoli of untreated cells. Most prominent among those structural elements are electron dense nodules of 100–170 m
μ size that appear to be composed primarily of proteins and that resist lead citrate staining unless they are stained first with uranyl acetate. Nodules of identical appearance may occur also outside nucleoli. These appear to be associated with the dispersed chromatin.
The mechanism by which adenosine exerts its effect on nucleoli is unknown. A meaningful interpretation is impossible at the present time, since little is known of its biochemical effects.</description><subject>Carcinoma - pathology</subject><subject>Cell Line - drug effects</subject><subject>Cell Nucleolus</subject><subject>Culture Techniques</subject><subject>Female</subject><subject>Humans</subject><subject>Microscopy, Electron</subject><subject>Nucleosides - pharmacology</subject><subject>Pepsin A - pharmacology</subject><subject>Ribonucleases - pharmacology</subject><subject>Trypsin - pharmacology</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>0022-5320</issn><issn>1878-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1LAzEQxYMotVb_hMKeRA-rk-xHsngQKX5BwYN6DmkysZHtbk02gv-9qS29ehoe770Z5kfIlMIVBVpfvwIwllcFgwsOlwKSzMUBGVPBRc6Ksjok433kmJyE8AlQUCrKERmVDeWNaMbk5sF1mIXBRz1Er9pML1X3gSFzXTYsMeuibjFupIkaTbb4yZTBrg-pdkqOrGoDnu3mhLw_3L_NnvL5y-Pz7G6e66KGIRc14EJbY4UtaFEybkVtEWvkyLkSrAaoSqZrqBpWCV41wnLTCMUhZYyxxYScb_euff8VMQxy5YLGtlUd9jFIUXKWmpCC1TaofR-CRyvX3q2U_5EU5Aaa_IMmN0QkB_kHTYrUm-4OxMUKzb61o5T8262P6ctvh14G7bBLPJxHPUjTu38u_AIkbnr_</recordid><startdate>197008</startdate><enddate>197008</enddate><creator>Recher, Louis</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197008</creationdate><title>Fine structural changes in the nucleus induced by adenosine</title><author>Recher, Louis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-860ebcfdf8f313427f86fee6e7e77a82600542c60592587598f7d98a70e6eddf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Carcinoma - pathology</topic><topic>Cell Line - drug effects</topic><topic>Cell Nucleolus</topic><topic>Culture Techniques</topic><topic>Female</topic><topic>Humans</topic><topic>Microscopy, Electron</topic><topic>Nucleosides - pharmacology</topic><topic>Pepsin A - pharmacology</topic><topic>Ribonucleases - pharmacology</topic><topic>Trypsin - pharmacology</topic><topic>Uterine Cervical Neoplasms - pathology</topic><toplevel>online_resources</toplevel><creatorcontrib>Recher, Louis</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ultrastructure research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Recher, Louis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fine structural changes in the nucleus induced by adenosine</atitle><jtitle>Journal of ultrastructure research</jtitle><addtitle>J Ultrastruct Res</addtitle><date>1970-08</date><risdate>1970</risdate><volume>32</volume><issue>3</issue><spage>212</spage><epage>225</epage><pages>212-225</pages><issn>0022-5320</issn><eissn>1878-2345</eissn><abstract>Cells of the ME-180 cell line, when treated with a nutrient medium that contains adenosine in a 2 m
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μ size that appear to be composed primarily of proteins and that resist lead citrate staining unless they are stained first with uranyl acetate. Nodules of identical appearance may occur also outside nucleoli. These appear to be associated with the dispersed chromatin.
The mechanism by which adenosine exerts its effect on nucleoli is unknown. A meaningful interpretation is impossible at the present time, since little is known of its biochemical effects.</abstract><cop>United States</cop><pub>Elsevier B.V</pub><pmid>4917989</pmid><doi>10.1016/S0022-5320(70)80002-8</doi><tpages>14</tpages></addata></record> |
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| ispartof | Journal of ultrastructure research, 1970-08, Vol.32 (3), p.212-225 |
| issn | 0022-5320 1878-2345 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84722580 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Carcinoma - pathology Cell Line - drug effects Cell Nucleolus Culture Techniques Female Humans Microscopy, Electron Nucleosides - pharmacology Pepsin A - pharmacology Ribonucleases - pharmacology Trypsin - pharmacology Uterine Cervical Neoplasms - pathology |
| title | Fine structural changes in the nucleus induced by adenosine |
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