Freeze-drying various attenuated strains of Pasteurella pestis
A method is described for the preparation of freeze-dried living attenuated Pasteurella pestis organisms in a medium suitable for the development of a vaccine for human use. Viability recovery rates of 50% or greater were obtained with the attenuated strains of P. pestis when concentrates prepared f...
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| Veröffentlicht in: | Cryobiology 1970-07, Vol.7 (1), p.40-43 |
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| container_end_page | 43 |
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| container_start_page | 40 |
| container_title | Cryobiology |
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| creator | Berman, Sanford Altieri, Patricia L. Groffinger, Albert Lowenthal, Joseph P. |
| description | A method is described for the preparation of freeze-dried living attenuated
Pasteurella pestis organisms in a medium suitable for the development of a vaccine for human use. Viability recovery rates of 50% or greater were obtained with the attenuated strains of
P. pestis when concentrates prepared from organisms harvested after 28-hr incubation were suspended in a sucrose-phosphate-glutamate solution containing 2.5% human serum albumin and freeze-dried. The freeze-dried organisms demonstrated no loss in viability after storage for 1 year at −20 °C. |
| doi_str_mv | 10.1016/0011-2240(70)90043-X |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0011-2240 |
| ispartof | Cryobiology, 1970-07, Vol.7 (1), p.40-43 |
| issn | 0011-2240 1090-2392 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84719397 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Culture Media Freeze Drying Plague Vaccine Suspensions Temperature Time Factors Yersinia pestis |
| title | Freeze-drying various attenuated strains of Pasteurella pestis |
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