Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ

[Display omitted] ► A real-time PCR method for the detection in situ of Anisakis pegreffii was developed. ► The ITS-2 of rDNA of A. pegreffii was used as molecular marker. ► The assay was capable of detecting 1/3 L3 in 30mg of fish tissue. To facilitate improved diagnosis and detection of the third...

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Veröffentlicht in:	Experimental parasitology 2011-02, Vol.127 (2), p.587-592
Hauptverfasser:	Fang, Wenzhen, Liu, Fan, Zhang, Shaolei, Lin, Junhua, Xu, Shisan, Luo, Damin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anisakiasis - diagnosis Anisakiasis - parasitology Anisakiasis - veterinary Anisakis Anisakis - genetics Anisakis - isolation & purification Anisakis pegreffii Biological and medical sciences DNA Primers DNA Probes DNA, Ribosomal DNA, Ribosomal Spacer - genetics Fish Diseases - diagnosis Fish Diseases - parasitology Fishes Fluorescent Dyes Food-borne parasitic zoonosis Fundamental and applied biological sciences. Psychology In situ Invertebrates Life cycle. Host-agent relationship. Pathogenesis Marine Nemathelminthia. Plathelmintha Nematode Polymerase Chain Reaction - methods Protozoa Reproducibility of Results Second internal transcribed spacer (ITS-2) Sensitivity and Specificity TaqMan real-time PCR
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