Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ
[Display omitted] ► A real-time PCR method for the detection in situ of Anisakis pegreffii was developed. ► The ITS-2 of rDNA of A. pegreffii was used as molecular marker. ► The assay was capable of detecting 1/3 L3 in 30mg of fish tissue. To facilitate improved diagnosis and detection of the third...
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| Veröffentlicht in: | Experimental parasitology 2011-02, Vol.127 (2), p.587-592 |
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| container_title | Experimental parasitology |
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| creator | Fang, Wenzhen Liu, Fan Zhang, Shaolei Lin, Junhua Xu, Shisan Luo, Damin |
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► A real-time PCR method for the detection in situ of Anisakis pegreffii was developed. ► The ITS-2 of rDNA of A. pegreffii was used as molecular marker. ► The assay was capable of detecting 1/3 L3 in 30mg of fish tissue.
To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single L3 in 30mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground. |
| doi_str_mv | 10.1016/j.exppara.2010.11.008 |
| format | Article |
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► A real-time PCR method for the detection in situ of Anisakis pegreffii was developed. ► The ITS-2 of rDNA of A. pegreffii was used as molecular marker. ► The assay was capable of detecting 1/3 L3 in 30mg of fish tissue.
To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single L3 in 30mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2010.11.008</identifier><identifier>PMID: 21110972</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Anisakiasis - diagnosis ; Anisakiasis - parasitology ; Anisakiasis - veterinary ; Anisakis ; Anisakis - genetics ; Anisakis - isolation & purification ; Anisakis pegreffii ; Biological and medical sciences ; DNA Primers ; DNA Probes ; DNA, Ribosomal ; DNA, Ribosomal Spacer - genetics ; Fish Diseases - diagnosis ; Fish Diseases - parasitology ; Fishes ; Fluorescent Dyes ; Food-borne parasitic zoonosis ; Fundamental and applied biological sciences. Psychology ; In situ ; Invertebrates ; Life cycle. Host-agent relationship. Pathogenesis ; Marine ; Nemathelminthia. Plathelmintha ; Nematode ; Polymerase Chain Reaction - methods ; Protozoa ; Reproducibility of Results ; Second internal transcribed spacer (ITS-2) ; Sensitivity and Specificity ; TaqMan real-time PCR</subject><ispartof>Experimental parasitology, 2011-02, Vol.127 (2), p.587-592</ispartof><rights>2010 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-c4f106c88acd1e643a9130067f4a41f88ed869ba8301c2df6d4468cfddd3f8843</citedby><cites>FETCH-LOGICAL-c427t-c4f106c88acd1e643a9130067f4a41f88ed869ba8301c2df6d4468cfddd3f8843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014489410003498$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23842273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21110972$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fang, Wenzhen</creatorcontrib><creatorcontrib>Liu, Fan</creatorcontrib><creatorcontrib>Zhang, Shaolei</creatorcontrib><creatorcontrib>Lin, Junhua</creatorcontrib><creatorcontrib>Xu, Shisan</creatorcontrib><creatorcontrib>Luo, Damin</creatorcontrib><title>Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>[Display omitted]
► A real-time PCR method for the detection in situ of Anisakis pegreffii was developed. ► The ITS-2 of rDNA of A. pegreffii was used as molecular marker. ► The assay was capable of detecting 1/3 L3 in 30mg of fish tissue.
To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single L3 in 30mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground.</description><subject>Animals</subject><subject>Anisakiasis - diagnosis</subject><subject>Anisakiasis - parasitology</subject><subject>Anisakiasis - veterinary</subject><subject>Anisakis</subject><subject>Anisakis - genetics</subject><subject>Anisakis - isolation & purification</subject><subject>Anisakis pegreffii</subject><subject>Biological and medical sciences</subject><subject>DNA Primers</subject><subject>DNA Probes</subject><subject>DNA, Ribosomal</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Fish Diseases - diagnosis</subject><subject>Fish Diseases - parasitology</subject><subject>Fishes</subject><subject>Fluorescent Dyes</subject><subject>Food-borne parasitic zoonosis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In situ</subject><subject>Invertebrates</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Marine</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>Nematode</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Protozoa</subject><subject>Reproducibility of Results</subject><subject>Second internal transcribed spacer (ITS-2)</subject><subject>Sensitivity and Specificity</subject><subject>TaqMan real-time PCR</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1PHCEYwHFianS1fgQbLo297MoDDMN4MZtNbZuYaJr2TBAeGtbZmRFmjH572ey2vbUXSODHS_6EnANbAAN1uV7gyzDYZBecbddgwZg-IDNgDZtzKZt3ZMYYyLnUjTwmJzmvWRHA5RE55gDF1XxG7pZdzPYxZjrgr4QhxHhFl_Rpst0YRzvGZ6ShnfqE2WHnkN6vvlObs32loU_U44hujH1HY0dzHKf35DDYNuPZfj4lP28-_1h9nd_effm2Wt7OneT1WMYATDmtrfOASgrbgGBM1UFaCUFr9Fo1D1YLBo77oLyUSrvgvRdlV4pTcrG7d0j904R5NJtYfti2tsN-ykZL1TDBRVPkp39KqEDVFedVVWi1oy71OZcaZkhxY9OrAWa21c3a7KubbXUDYErTcu7D_onpYYP-z6nfmQv4uAc2O9uGZDsX818ntOS8FsVd7xyWdM8Rk8kubrP7mEpn4_v4n6-8AS6IokE</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Fang, Wenzhen</creator><creator>Liu, Fan</creator><creator>Zhang, Shaolei</creator><creator>Lin, Junhua</creator><creator>Xu, Shisan</creator><creator>Luo, Damin</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20110201</creationdate><title>Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ</title><author>Fang, Wenzhen ; Liu, Fan ; Zhang, Shaolei ; Lin, Junhua ; Xu, Shisan ; Luo, Damin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-c4f106c88acd1e643a9130067f4a41f88ed869ba8301c2df6d4468cfddd3f8843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Anisakiasis - diagnosis</topic><topic>Anisakiasis - parasitology</topic><topic>Anisakiasis - veterinary</topic><topic>Anisakis</topic><topic>Anisakis - genetics</topic><topic>Anisakis - isolation & purification</topic><topic>Anisakis pegreffii</topic><topic>Biological and medical sciences</topic><topic>DNA Primers</topic><topic>DNA Probes</topic><topic>DNA, Ribosomal</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Fish Diseases - diagnosis</topic><topic>Fish Diseases - parasitology</topic><topic>Fishes</topic><topic>Fluorescent Dyes</topic><topic>Food-borne parasitic zoonosis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In situ</topic><topic>Invertebrates</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Marine</topic><topic>Nemathelminthia. Plathelmintha</topic><topic>Nematode</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Protozoa</topic><topic>Reproducibility of Results</topic><topic>Second internal transcribed spacer (ITS-2)</topic><topic>Sensitivity and Specificity</topic><topic>TaqMan real-time PCR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fang, Wenzhen</creatorcontrib><creatorcontrib>Liu, Fan</creatorcontrib><creatorcontrib>Zhang, Shaolei</creatorcontrib><creatorcontrib>Lin, Junhua</creatorcontrib><creatorcontrib>Xu, Shisan</creatorcontrib><creatorcontrib>Luo, Damin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fang, Wenzhen</au><au>Liu, Fan</au><au>Zhang, Shaolei</au><au>Lin, Junhua</au><au>Xu, Shisan</au><au>Luo, Damin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>127</volume><issue>2</issue><spage>587</spage><epage>592</epage><pages>587-592</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>[Display omitted]
► A real-time PCR method for the detection in situ of Anisakis pegreffii was developed. ► The ITS-2 of rDNA of A. pegreffii was used as molecular marker. ► The assay was capable of detecting 1/3 L3 in 30mg of fish tissue.
To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single L3 in 30mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>21110972</pmid><doi>10.1016/j.exppara.2010.11.008</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Anisakiasis - diagnosis Anisakiasis - parasitology Anisakiasis - veterinary Anisakis Anisakis - genetics Anisakis - isolation & purification Anisakis pegreffii Biological and medical sciences DNA Primers DNA Probes DNA, Ribosomal DNA, Ribosomal Spacer - genetics Fish Diseases - diagnosis Fish Diseases - parasitology Fishes Fluorescent Dyes Food-borne parasitic zoonosis Fundamental and applied biological sciences. Psychology In situ Invertebrates Life cycle. Host-agent relationship. Pathogenesis Marine Nemathelminthia. Plathelmintha Nematode Polymerase Chain Reaction - methods Protozoa Reproducibility of Results Second internal transcribed spacer (ITS-2) Sensitivity and Specificity TaqMan real-time PCR |
| title | Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ |
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