Outer Chloroplast Envelope Protein OEP16-1 for Plastid Import of NADPH:Protochlorophyllide Oxidoreductase A in Arabidopsis thaliana
The outer plastid envelope protein OEP16-1 was previously identified as an amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA). Reverse genetic approaches used to dissect these mutually not exclusive functions of OEP16-1 in planta have le...
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| Veröffentlicht in: | Plant and cell physiology 2011-01, Vol.52 (1), p.96-111 |
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| container_title | Plant and cell physiology |
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| creator | Samol, Iga Rossig, Claudia Buhr, Frank Springer, Armin Pollmann, Stephan Lahroussi, Abder von Wettstein, Diter Reinbothe, Christiane Reinbothe, Steffen |
| description | The outer plastid envelope protein OEP16-1 was previously identified as an amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA). Reverse genetic approaches used to dissect these mutually not exclusive functions of OEP16-1 in planta have led to descriptions of different phenotypes resulting from the presence of several mutant lines in the SALK_024018 seed stock. In addition to the T-DNA insertion in the AtOEP16-1 gene, lines were purified that contain two additional T-DNA insertions and as yet unidentified point mutations. In a first attempt to resolve the genetic basis of four different lines in the SALK_024018 seed stock, we used genetic transformation with the OEP16-1 cDNA and segregation analyses after crossing out presumed point mutations. We show that AtOEP16-1 is involved in PORA precursor import and by virtue of this activity confers photoprotection onto etiolated seedlings during greening. |
| doi_str_mv | 10.1093/pcp/pcq177 |
| format | Article |
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We show that AtOEP16-1 is involved in PORA precursor import and by virtue of this activity confers photoprotection onto etiolated seedlings during greening.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Genetic Complementation Test</subject><subject>Ion Channels - genetics</subject><subject>Ion Channels - metabolism</subject><subject>Mutation</subject><subject>Plastids - metabolism</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1rGzEQhkVJaJy0l_6AVLdAYJPRar-Um3HdJmBiQ-uz0GpHsYpsbaTdkpz7xyuzaQ5ixPDMy8xDyBcGNwwEv-11n94zq-sPZMaKmmUCSn5CZgA8z6Bu2Bk5j_E3QPpz-EjO8jTXlGU1I3_X44CBLnbOB987FQe6PPxB53ukm-AHtAe6Xm5YlTFqfKCbI2I7-rDvfRioN_Rx_m1zf3dkvZ5Sdq_O2Q7p-sV2PmA36kFFpHOasuZBtanbRxvpsFPOqoP6RE6NchE_v9ULsv2-_LW4z1brHw-L-SrTBW-GDDWwtqg5z4FpYzoEDSWqWhhe5YaVJjet0po3nJclh44JrkCYCplo2jxn_IJcTbl98M8jxkHubdTonDqgH6NsiqoRNTQikdcTqYOPMaCRfbB7FV4lA3l0LpNzOTlP8OVb7NjusXtH_0tOwNcJMMpL9RRslNuf6QgOacWiFDX_B-rMiAU</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Samol, Iga</creator><creator>Rossig, Claudia</creator><creator>Buhr, Frank</creator><creator>Springer, Armin</creator><creator>Pollmann, Stephan</creator><creator>Lahroussi, Abder</creator><creator>von Wettstein, Diter</creator><creator>Reinbothe, Christiane</creator><creator>Reinbothe, Steffen</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110101</creationdate><title>Outer Chloroplast Envelope Protein OEP16-1 for Plastid Import of NADPH:Protochlorophyllide Oxidoreductase A in Arabidopsis thaliana</title><author>Samol, Iga ; Rossig, Claudia ; Buhr, Frank ; Springer, Armin ; Pollmann, Stephan ; Lahroussi, Abder ; von Wettstein, Diter ; Reinbothe, Christiane ; Reinbothe, Steffen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-ec01b4733201cffde0c05ea79f362f15f2fbacc38335530d193a09f6e198b2213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Genetic Complementation Test</topic><topic>Ion Channels - genetics</topic><topic>Ion Channels - metabolism</topic><topic>Mutation</topic><topic>Plastids - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Samol, Iga</creatorcontrib><creatorcontrib>Rossig, Claudia</creatorcontrib><creatorcontrib>Buhr, Frank</creatorcontrib><creatorcontrib>Springer, Armin</creatorcontrib><creatorcontrib>Pollmann, Stephan</creatorcontrib><creatorcontrib>Lahroussi, Abder</creatorcontrib><creatorcontrib>von Wettstein, Diter</creatorcontrib><creatorcontrib>Reinbothe, Christiane</creatorcontrib><creatorcontrib>Reinbothe, Steffen</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Samol, Iga</au><au>Rossig, Claudia</au><au>Buhr, Frank</au><au>Springer, Armin</au><au>Pollmann, Stephan</au><au>Lahroussi, Abder</au><au>von Wettstein, Diter</au><au>Reinbothe, Christiane</au><au>Reinbothe, Steffen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Outer Chloroplast Envelope Protein OEP16-1 for Plastid Import of NADPH:Protochlorophyllide Oxidoreductase A in Arabidopsis thaliana</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>52</volume><issue>1</issue><spage>96</spage><epage>111</epage><pages>96-111</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>The outer plastid envelope protein OEP16-1 was previously identified as an amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA). 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| ispartof | Plant and cell physiology, 2011-01, Vol.52 (1), p.96-111 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Genetic Complementation Test Ion Channels - genetics Ion Channels - metabolism Mutation Plastids - metabolism |
| title | Outer Chloroplast Envelope Protein OEP16-1 for Plastid Import of NADPH:Protochlorophyllide Oxidoreductase A in Arabidopsis thaliana |
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