Factors Influencing Bioassay of Porcine and Human Calcitonin
Several factors which affect the sensitivity of calcitonin bioassay were investigated. As to the assay animals, Sprague-Dawley and Holtzman rats were more sensitive than Wistar rats. In mice, C3H III strain gave better response to calcitonin than CF 1 and ddN strains. In each strain, younger rats we...
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| Veröffentlicht in: | Endocrinologia Japonica 1970, Vol.17(2), pp.157-164 |
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| container_title | Endocrinologia Japonica |
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| creator | MORII, HIROTOSHI IBAYASHI, HIROSHI SHIMAZAWA, EIICHIRO ASANO, HIDEICHI |
| description | Several factors which affect the sensitivity of calcitonin bioassay were investigated. As to the assay animals, Sprague-Dawley and Holtzman rats were more sensitive than Wistar rats. In mice, C3H III strain gave better response to calcitonin than CF 1 and ddN strains. In each strain, younger rats were more sensitive than older ones. It was also demonstrated that the longer was the period of low calcium diet, the more remarkable was the response of the Wistar rats to calcitonin. In Wistar rats, phosphate in drinking water improved the sensitivity of the assay and intravenous administration of calcitonin produced steeper dose-response curve than subcutaneous administration. When 3 to 4-week-old Sprague-Dawley rats were utilized, the hypocalcemic acitivity was detected in 25 to 66 mg of wet weight of human thyroid glands, while 5-week-old Sprague-Dawley rats were not so sensitive as to be able to detect hypocalcemic activity in 100mg of wet weight of human thyroid glands. Two-week-old Holtzman rats were so sensitive that we detected hypocalcemic activity in 1mg of wet weight of a metastatic lymph node of the medullary thyroid carcinoma. |
| doi_str_mv | 10.1507/endocrj1954.17.157 |
| format | Article |
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As to the assay animals, Sprague-Dawley and Holtzman rats were more sensitive than Wistar rats. In mice, C3H III strain gave better response to calcitonin than CF 1 and ddN strains. In each strain, younger rats were more sensitive than older ones. It was also demonstrated that the longer was the period of low calcium diet, the more remarkable was the response of the Wistar rats to calcitonin. In Wistar rats, phosphate in drinking water improved the sensitivity of the assay and intravenous administration of calcitonin produced steeper dose-response curve than subcutaneous administration. When 3 to 4-week-old Sprague-Dawley rats were utilized, the hypocalcemic acitivity was detected in 25 to 66 mg of wet weight of human thyroid glands, while 5-week-old Sprague-Dawley rats were not so sensitive as to be able to detect hypocalcemic activity in 100mg of wet weight of human thyroid glands. Two-week-old Holtzman rats were so sensitive that we detected hypocalcemic activity in 1mg of wet weight of a metastatic lymph node of the medullary thyroid carcinoma.</description><identifier>ISSN: 0013-7219</identifier><identifier>EISSN: 2185-6370</identifier><identifier>DOI: 10.1507/endocrj1954.17.157</identifier><identifier>PMID: 5468814</identifier><language>eng</language><publisher>Japan: The Japan Endocrine Society</publisher><subject>Age Factors ; Animals ; Biological Assay ; Calcitonin - analysis ; Calcium - blood ; Calcium - pharmacology ; Diet ; Female ; Humans ; Injections, Intravenous ; Injections, Subcutaneous ; Lymphatic Metastasis - diagnosis ; Male ; Mice ; Phosphates - pharmacology ; Rats ; Species Specificity ; Swine ; Thyroid Neoplasms - diagnosis</subject><ispartof>Endocrinologia Japonica, 1970, Vol.17(2), pp.157-164</ispartof><rights>The Japan Endocrine Society</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-6c8e654194e7b5f52deb6265b4a33e81e3f4b42a30e3c743d684db7d2e67915c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5468814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MORII, HIROTOSHI</creatorcontrib><creatorcontrib>IBAYASHI, HIROSHI</creatorcontrib><creatorcontrib>SHIMAZAWA, EIICHIRO</creatorcontrib><creatorcontrib>ASANO, HIDEICHI</creatorcontrib><title>Factors Influencing Bioassay of Porcine and Human Calcitonin</title><title>Endocrinologia Japonica</title><addtitle>Endocrinol Japon</addtitle><description>Several factors which affect the sensitivity of calcitonin bioassay were investigated. As to the assay animals, Sprague-Dawley and Holtzman rats were more sensitive than Wistar rats. In mice, C3H III strain gave better response to calcitonin than CF 1 and ddN strains. In each strain, younger rats were more sensitive than older ones. It was also demonstrated that the longer was the period of low calcium diet, the more remarkable was the response of the Wistar rats to calcitonin. In Wistar rats, phosphate in drinking water improved the sensitivity of the assay and intravenous administration of calcitonin produced steeper dose-response curve than subcutaneous administration. When 3 to 4-week-old Sprague-Dawley rats were utilized, the hypocalcemic acitivity was detected in 25 to 66 mg of wet weight of human thyroid glands, while 5-week-old Sprague-Dawley rats were not so sensitive as to be able to detect hypocalcemic activity in 100mg of wet weight of human thyroid glands. Two-week-old Holtzman rats were so sensitive that we detected hypocalcemic activity in 1mg of wet weight of a metastatic lymph node of the medullary thyroid carcinoma.</description><subject>Age Factors</subject><subject>Animals</subject><subject>Biological Assay</subject><subject>Calcitonin - analysis</subject><subject>Calcium - blood</subject><subject>Calcium - pharmacology</subject><subject>Diet</subject><subject>Female</subject><subject>Humans</subject><subject>Injections, Intravenous</subject><subject>Injections, Subcutaneous</subject><subject>Lymphatic Metastasis - diagnosis</subject><subject>Male</subject><subject>Mice</subject><subject>Phosphates - pharmacology</subject><subject>Rats</subject><subject>Species Specificity</subject><subject>Swine</subject><subject>Thyroid Neoplasms - diagnosis</subject><issn>0013-7219</issn><issn>2185-6370</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUEtLw0AQXkSppfYPCEJO3lL3vQl40WIfUNCDnsNmM6kpyW7dTQ7-e1caijiHGfheDB9CtwQviMDqAWzljD-QXPAFURFTF2hKSSZSyRS-RFOMCUsVJfk1modwwHEklYrjCZoILrOM8Cl6XGnTOx-Sra3bAaxp7D55bpwOQX8nrk7enI8YJNpWyWbotE2WujVN72xjb9BVrdsA8_HO0Mfq5X25SXev6-3yaZcaQfM-lSYDKTjJOahS1IJWUMZPRMk1Y5ARYDUvOdUMAzOKs0pmvCpVRUGqnAjDZuj-lHv07muA0BddEwy0rbbghlBkXCqGCY5CehIa70LwUBdH33TafxcEF7-tFX9aK4iKmIqmuzF9KDuozpaxo8ivT_wh9HoPZ177vjEt_I-k4xLqrDCf2kcZ-wH1f4JO</recordid><startdate>19700101</startdate><enddate>19700101</enddate><creator>MORII, HIROTOSHI</creator><creator>IBAYASHI, HIROSHI</creator><creator>SHIMAZAWA, EIICHIRO</creator><creator>ASANO, HIDEICHI</creator><general>The Japan Endocrine Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19700101</creationdate><title>Factors Influencing Bioassay of Porcine and Human Calcitonin</title><author>MORII, HIROTOSHI ; IBAYASHI, HIROSHI ; SHIMAZAWA, EIICHIRO ; ASANO, HIDEICHI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-6c8e654194e7b5f52deb6265b4a33e81e3f4b42a30e3c743d684db7d2e67915c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Age Factors</topic><topic>Animals</topic><topic>Biological Assay</topic><topic>Calcitonin - analysis</topic><topic>Calcium - blood</topic><topic>Calcium - pharmacology</topic><topic>Diet</topic><topic>Female</topic><topic>Humans</topic><topic>Injections, Intravenous</topic><topic>Injections, Subcutaneous</topic><topic>Lymphatic Metastasis - diagnosis</topic><topic>Male</topic><topic>Mice</topic><topic>Phosphates - pharmacology</topic><topic>Rats</topic><topic>Species Specificity</topic><topic>Swine</topic><topic>Thyroid Neoplasms - diagnosis</topic><toplevel>online_resources</toplevel><creatorcontrib>MORII, HIROTOSHI</creatorcontrib><creatorcontrib>IBAYASHI, HIROSHI</creatorcontrib><creatorcontrib>SHIMAZAWA, EIICHIRO</creatorcontrib><creatorcontrib>ASANO, HIDEICHI</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinologia Japonica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MORII, HIROTOSHI</au><au>IBAYASHI, HIROSHI</au><au>SHIMAZAWA, EIICHIRO</au><au>ASANO, HIDEICHI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Factors Influencing Bioassay of Porcine and Human Calcitonin</atitle><jtitle>Endocrinologia Japonica</jtitle><addtitle>Endocrinol Japon</addtitle><date>1970-01-01</date><risdate>1970</risdate><volume>17</volume><issue>2</issue><spage>157</spage><epage>164</epage><pages>157-164</pages><issn>0013-7219</issn><eissn>2185-6370</eissn><abstract>Several factors which affect the sensitivity of calcitonin bioassay were investigated. As to the assay animals, Sprague-Dawley and Holtzman rats were more sensitive than Wistar rats. In mice, C3H III strain gave better response to calcitonin than CF 1 and ddN strains. In each strain, younger rats were more sensitive than older ones. It was also demonstrated that the longer was the period of low calcium diet, the more remarkable was the response of the Wistar rats to calcitonin. In Wistar rats, phosphate in drinking water improved the sensitivity of the assay and intravenous administration of calcitonin produced steeper dose-response curve than subcutaneous administration. When 3 to 4-week-old Sprague-Dawley rats were utilized, the hypocalcemic acitivity was detected in 25 to 66 mg of wet weight of human thyroid glands, while 5-week-old Sprague-Dawley rats were not so sensitive as to be able to detect hypocalcemic activity in 100mg of wet weight of human thyroid glands. Two-week-old Holtzman rats were so sensitive that we detected hypocalcemic activity in 1mg of wet weight of a metastatic lymph node of the medullary thyroid carcinoma.</abstract><cop>Japan</cop><pub>The Japan Endocrine Society</pub><pmid>5468814</pmid><doi>10.1507/endocrj1954.17.157</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Endocrinologia Japonica, 1970, Vol.17(2), pp.157-164 |
| issn | 0013-7219 2185-6370 |
| language | eng |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Age Factors Animals Biological Assay Calcitonin - analysis Calcium - blood Calcium - pharmacology Diet Female Humans Injections, Intravenous Injections, Subcutaneous Lymphatic Metastasis - diagnosis Male Mice Phosphates - pharmacology Rats Species Specificity Swine Thyroid Neoplasms - diagnosis |
| title | Factors Influencing Bioassay of Porcine and Human Calcitonin |
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