Freezability of Bull Semen in Centrifuged or Dialyzed and Reconstituted Buffered Egg Yolk Extenders
Bull spermatozoa were frozen with equal success in a 0.25 m Tris-buffered extender containing either the supernatant or precipitated portion of centrifuged egg yolk. Washing the precipitate with distilled water to remove additional ions and other water-soluble materials had little effect on the osmo...
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| Veröffentlicht in: | Journal of dairy science 1967-09, Vol.50 (9), p.1481-1485 |
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| container_title | Journal of dairy science |
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| creator | Yassen, A.M. Foote, R.H. |
| description | Bull spermatozoa were frozen with equal success in a 0.25 m Tris-buffered extender containing either the supernatant or precipitated portion of centrifuged egg yolk. Washing the precipitate with distilled water to remove additional ions and other water-soluble materials had little effect on the osmotic pressure of the washed egg yolk precipitate reconstituted with Tris, but viability of spermatozoa both before and after freezing was reduced.
Egg yolk was dialyzed against distilled water until the Na and K levels were reduced to 12 and 10μg per ml, respectively. Different proportions of dialyzed and non-dialyzed egg yolk were combined with Tris buffer, and sperm cell survival following freezing was higher than in the Tris-yolk control (P |
| doi_str_mv | 10.3168/jds.S0022-0302(67)87652-5 |
| format | Article |
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Egg yolk was dialyzed against distilled water until the Na and K levels were reduced to 12 and 10μg per ml, respectively. Different proportions of dialyzed and non-dialyzed egg yolk were combined with Tris buffer, and sperm cell survival following freezing was higher than in the Tris-yolk control (P<.01). Post-thaw motility of spermatozoa ranged from 80 to 90% of the prefreeze motility. The results indicate that high survival of bull spermatozoa can be achieved with an extender low in all ions except those supplied by Tris.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.S0022-0302(67)87652-5</identifier><identifier>PMID: 6070688</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cattle ; Centrifugation ; Dialysis ; Egg Yolk ; Female ; Freezing ; Male ; Preservation, Biological ; Semen</subject><ispartof>Journal of dairy science, 1967-09, Vol.50 (9), p.1481-1485</ispartof><rights>1967 American Dairy Science Association</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-72b7524e7d4ed8cfe0cfb8f8950db7ff2e340c3581c241a666f64b1df2e1b3d03</citedby><cites>FETCH-LOGICAL-c482t-72b7524e7d4ed8cfe0cfb8f8950db7ff2e340c3581c241a666f64b1df2e1b3d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3168/jds.S0022-0302(67)87652-5$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27869,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6070688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yassen, A.M.</creatorcontrib><creatorcontrib>Foote, R.H.</creatorcontrib><title>Freezability of Bull Semen in Centrifuged or Dialyzed and Reconstituted Buffered Egg Yolk Extenders</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>Bull spermatozoa were frozen with equal success in a 0.25 m Tris-buffered extender containing either the supernatant or precipitated portion of centrifuged egg yolk. Washing the precipitate with distilled water to remove additional ions and other water-soluble materials had little effect on the osmotic pressure of the washed egg yolk precipitate reconstituted with Tris, but viability of spermatozoa both before and after freezing was reduced.
Egg yolk was dialyzed against distilled water until the Na and K levels were reduced to 12 and 10μg per ml, respectively. Different proportions of dialyzed and non-dialyzed egg yolk were combined with Tris buffer, and sperm cell survival following freezing was higher than in the Tris-yolk control (P<.01). Post-thaw motility of spermatozoa ranged from 80 to 90% of the prefreeze motility. The results indicate that high survival of bull spermatozoa can be achieved with an extender low in all ions except those supplied by Tris.</description><subject>Animals</subject><subject>Cattle</subject><subject>Centrifugation</subject><subject>Dialysis</subject><subject>Egg Yolk</subject><subject>Female</subject><subject>Freezing</subject><subject>Male</subject><subject>Preservation, Biological</subject><subject>Semen</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1967</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>K30</sourceid><recordid>eNqNkVFvFCEUhYnR1LX6E0wwJkYfpgIDDPNo162aNDGx-uATmYHLlpUZWphp3f562e6mJj75xAW-e-_JOQi9ouSkplK939h8ckEIYxWpCXsrm3eqkYJV4hFaUMFEVdNWPUaLB-QpepbzplwpI-IIHUnSEKnUApmzBHDX9T74aYujw6dzCPgCBhixH_ESxil5N6_B4pjwR9-F7V2pu9Hib2DimCc_zVN5OZ2dg1SK1XqNf8bwC69-TzBaSPk5euK6kOHF4TxGP85W35efq_Ovn74sP5xXhis2VQ3rG8E4NJaDVcYBMa5XTrWC2L5xjkHNiamFooZx2kkpneQ9teWD9rUl9TF6s597leL1DHnSg88GQuhGiHPWiksh6mYHvv4H3MQ5jUWbpqpuleCM8UK1e8qkmHMCp6-SH7q01ZToXQy6xKDvY9A7j7Vs9H0MWpTel4cNcz-Afeg8-P5X6qVfX976BDoPXQiFprupguhWU65oAZd7EIpxNx6SzsbDaMCWJjNpG_1_yPkDuYGnjg</recordid><startdate>196709</startdate><enddate>196709</enddate><creator>Yassen, A.M.</creator><creator>Foote, R.H.</creator><general>Elsevier Inc</general><general>Am Dairy Sci Assoc</general><general>American Dairy Science Association</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7WH</scope><scope>K30</scope><scope>PAAUG</scope><scope>PAWHS</scope><scope>PAWZZ</scope><scope>PAXOH</scope><scope>PBHAV</scope><scope>PBQSW</scope><scope>PBYQZ</scope><scope>PCIWU</scope><scope>PCMID</scope><scope>PCZJX</scope><scope>PDGRG</scope><scope>PDWWI</scope><scope>PETMR</scope><scope>PFVGT</scope><scope>PGXDX</scope><scope>PIHIL</scope><scope>PISVA</scope><scope>PJCTQ</scope><scope>PJTMS</scope><scope>PLCHJ</scope><scope>PMHAD</scope><scope>PNQDJ</scope><scope>POUND</scope><scope>PPLAD</scope><scope>PQAPC</scope><scope>PQCAN</scope><scope>PQCMW</scope><scope>PQEME</scope><scope>PQHKH</scope><scope>PQMID</scope><scope>PQNCT</scope><scope>PQNET</scope><scope>PQSCT</scope><scope>PQSET</scope><scope>PSVJG</scope><scope>PVMQY</scope><scope>PZGFC</scope><scope>7X8</scope></search><sort><creationdate>196709</creationdate><title>Freezability of Bull Semen in Centrifuged or Dialyzed and Reconstituted Buffered Egg Yolk Extenders</title><author>Yassen, A.M. ; Foote, R.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-72b7524e7d4ed8cfe0cfb8f8950db7ff2e340c3581c241a666f64b1df2e1b3d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1967</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Centrifugation</topic><topic>Dialysis</topic><topic>Egg Yolk</topic><topic>Female</topic><topic>Freezing</topic><topic>Male</topic><topic>Preservation, Biological</topic><topic>Semen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yassen, A.M.</creatorcontrib><creatorcontrib>Foote, R.H.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Periodicals Index Online Segment 50</collection><collection>Periodicals Index Online</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - West</collection><collection>Primary Sources Access (Plan D) - International</collection><collection>Primary Sources Access & Build (Plan A) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Midwest</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Northeast</collection><collection>Primary Sources Access (Plan D) - Southeast</collection><collection>Primary Sources Access (Plan D) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Southeast</collection><collection>Primary Sources Access (Plan D) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - UK / I</collection><collection>Primary Sources Access (Plan D) - Canada</collection><collection>Primary Sources Access (Plan D) - EMEALA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - International</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - International</collection><collection>Primary Sources Access (Plan D) - West</collection><collection>Periodicals Index Online Segments 1-50</collection><collection>Primary Sources Access (Plan D) - APAC</collection><collection>Primary Sources Access (Plan D) - Midwest</collection><collection>Primary Sources Access (Plan D) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Canada</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - EMEALA</collection><collection>Primary Sources Access & Build (Plan A) - APAC</collection><collection>Primary Sources Access & Build (Plan A) - Canada</collection><collection>Primary Sources Access & Build (Plan A) - West</collection><collection>Primary Sources Access & Build (Plan A) - EMEALA</collection><collection>Primary Sources Access (Plan D) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - Midwest</collection><collection>Primary Sources Access & Build (Plan A) - North Central</collection><collection>Primary Sources Access & Build (Plan A) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - Southeast</collection><collection>Primary Sources Access (Plan D) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - APAC</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - MEA</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yassen, A.M.</au><au>Foote, R.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Freezability of Bull Semen in Centrifuged or Dialyzed and Reconstituted Buffered Egg Yolk Extenders</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>1967-09</date><risdate>1967</risdate><volume>50</volume><issue>9</issue><spage>1481</spage><epage>1485</epage><pages>1481-1485</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><abstract>Bull spermatozoa were frozen with equal success in a 0.25 m Tris-buffered extender containing either the supernatant or precipitated portion of centrifuged egg yolk. Washing the precipitate with distilled water to remove additional ions and other water-soluble materials had little effect on the osmotic pressure of the washed egg yolk precipitate reconstituted with Tris, but viability of spermatozoa both before and after freezing was reduced.
Egg yolk was dialyzed against distilled water until the Na and K levels were reduced to 12 and 10μg per ml, respectively. Different proportions of dialyzed and non-dialyzed egg yolk were combined with Tris buffer, and sperm cell survival following freezing was higher than in the Tris-yolk control (P<.01). Post-thaw motility of spermatozoa ranged from 80 to 90% of the prefreeze motility. The results indicate that high survival of bull spermatozoa can be achieved with an extender low in all ions except those supplied by Tris.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6070688</pmid><doi>10.3168/jds.S0022-0302(67)87652-5</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Periodicals Index Online |
| subjects | Animals Cattle Centrifugation Dialysis Egg Yolk Female Freezing Male Preservation, Biological Semen |
| title | Freezability of Bull Semen in Centrifuged or Dialyzed and Reconstituted Buffered Egg Yolk Extenders |
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