Purification and properties of follicle-stimulating and luteinizing hormones from horse pituitary glands

A method is described for the purification of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from horse pituitary glands. The glands were extracted twice in 40% ethanol, and the active protein was recovered by acetone precipitation. Inactive protein was removed by dialysis of the ac...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1970-07, Vol.139 (1), p.45-58
Hauptverfasser:	Braselton, W.E., McShan, W.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetone Animals Ascorbic Acid Biological Assay Chemical Precipitation Chromatography, Gel Chromatography, Ion Exchange Dialysis Electrophoresis, Disc Ethanol Female Follicle Stimulating Hormone - isolation & purification Horses Hydrogen-Ion Concentration Immunodiffusion Immunoelectrophoresis Isoelectric Focusing Luteinizing Hormone - isolation & purification Male Methods Molecular Weight Organ Size Ovary - drug effects Phosphorous Acids Pituitary Gland - analysis Prostate - anatomy & histology Prostate - drug effects Rabbits Rats Sodium Chloride Ultracentrifugation
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