Characterization and Isolation of Specific Renal Tubular Epithelial Antigens
Two renal tubular epithelial specific antigens were found to be membrane-bound and localized to the brush border of cells of the proximal convoluted tubules of the rat. Cross-reacting or identical antigens were also identified in the brush border of the small bowel mucosa. Both antigens were isolate...
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| Veröffentlicht in: | The Journal of immunology (1950) 1967-12, Vol.99 (6), p.1199-1210 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 99 |
| creator | Edgington, Thomas S Glassock, Richard J Watson, J. Ian Dixon, Frank J |
| description | Two renal tubular epithelial specific antigens were found to be membrane-bound and localized to the brush border of cells of the proximal convoluted tubules of the rat. Cross-reacting or identical antigens were also identified in the brush border of the small bowel mucosa. Both antigens were isolated biochemically and one was highly purified. One antigen, renal tubular epithelium (RTE)-α4, appears to be a lipoprotein, so20w of 28S, and the other a protein, so20w of 9.8S. RTE-α3 is immunogenic but is masked in situ so that direct interaction with humoral antibody does not occur. This antigen does appear in the serum following tubular epithelial injury. Neither of the isolated RTE-specific antigens is the pathogenetic antigen responsible for experimental allergic glomerulonephritis induced with tubular antigen. |
| doi_str_mv | 10.4049/jimmunol.99.6.1199 |
| format | Article |
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Ian ; Dixon, Frank J</creator><creatorcontrib>Edgington, Thomas S ; Glassock, Richard J ; Watson, J. Ian ; Dixon, Frank J</creatorcontrib><description>Two renal tubular epithelial specific antigens were found to be membrane-bound and localized to the brush border of cells of the proximal convoluted tubules of the rat. Cross-reacting or identical antigens were also identified in the brush border of the small bowel mucosa. Both antigens were isolated biochemically and one was highly purified. One antigen, renal tubular epithelium (RTE)-α4, appears to be a lipoprotein, so20w of 28S, and the other a protein, so20w of 9.8S. RTE-α3 is immunogenic but is masked in situ so that direct interaction with humoral antibody does not occur. This antigen does appear in the serum following tubular epithelial injury. Neither of the isolated RTE-specific antigens is the pathogenetic antigen responsible for experimental allergic glomerulonephritis induced with tubular antigen.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.99.6.1199</identifier><identifier>PMID: 4862890</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Animals ; Antigens - analysis ; Chromatography, Ion Exchange ; Epithelium ; Fluorescent Antibody Technique ; Glomerulonephritis - immunology ; Hypersensitivity ; Immunoelectrophoresis ; Kidney Tubules - immunology ; Male ; Microscopy, Fluorescence ; Rabbits ; Rats ; Ultracentrifugation</subject><ispartof>The Journal of immunology (1950), 1967-12, Vol.99 (6), p.1199-1210</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-59ac2d1d486654b6154548588f4cbca936f1f4e3ba1b17d5b099d4eafdbd0c9f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4862890$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Edgington, Thomas S</creatorcontrib><creatorcontrib>Glassock, Richard J</creatorcontrib><creatorcontrib>Watson, J. Ian</creatorcontrib><creatorcontrib>Dixon, Frank J</creatorcontrib><title>Characterization and Isolation of Specific Renal Tubular Epithelial Antigens</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Two renal tubular epithelial specific antigens were found to be membrane-bound and localized to the brush border of cells of the proximal convoluted tubules of the rat. Cross-reacting or identical antigens were also identified in the brush border of the small bowel mucosa. Both antigens were isolated biochemically and one was highly purified. One antigen, renal tubular epithelium (RTE)-α4, appears to be a lipoprotein, so20w of 28S, and the other a protein, so20w of 9.8S. RTE-α3 is immunogenic but is masked in situ so that direct interaction with humoral antibody does not occur. This antigen does appear in the serum following tubular epithelial injury. Neither of the isolated RTE-specific antigens is the pathogenetic antigen responsible for experimental allergic glomerulonephritis induced with tubular antigen.</description><subject>Animals</subject><subject>Antigens - analysis</subject><subject>Chromatography, Ion Exchange</subject><subject>Epithelium</subject><subject>Fluorescent Antibody Technique</subject><subject>Glomerulonephritis - immunology</subject><subject>Hypersensitivity</subject><subject>Immunoelectrophoresis</subject><subject>Kidney Tubules - immunology</subject><subject>Male</subject><subject>Microscopy, Fluorescence</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Ultracentrifugation</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1967</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkF9LwzAUxYMoc06_gCD0ybfWpE2y5nGMqYOBoPM5JGmyZqR_TFqKfno7Nt3T5V7OOffwA-AewQRDzJ72tqr6unEJYwlNEGLsAkwRITCmFNJLMIUwTWM0p_NrcBPCHkJIYYonYIJzmuYMTsFmWQovVKe9_RGdbepI1EW0Do07bo2JPlqtrLEqete1cNG2l70TPlq1tiu1s-NpUXd2p-twC66McEHfneYMfD6vtsvXePP2sl4uNrHCGetiwoRKC1SMJSjBkiKCCc5JnhuspBIsowYZrDMpkETzgkjIWIG1MIUsoGImm4HHY27rm69eh45XNijtnKh10weeY8LyjMJRmB6FyjcheG14620l_DdHkB8Q8j-EnDFO-QHhaHo4pfey0sW_5cTs_L20u3KwXvNQCedGNeLDMJyDfgFhsX2C</recordid><startdate>196712</startdate><enddate>196712</enddate><creator>Edgington, Thomas S</creator><creator>Glassock, Richard J</creator><creator>Watson, J. 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Ian</au><au>Dixon, Frank J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and Isolation of Specific Renal Tubular Epithelial Antigens</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1967-12</date><risdate>1967</risdate><volume>99</volume><issue>6</issue><spage>1199</spage><epage>1210</epage><pages>1199-1210</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Two renal tubular epithelial specific antigens were found to be membrane-bound and localized to the brush border of cells of the proximal convoluted tubules of the rat. Cross-reacting or identical antigens were also identified in the brush border of the small bowel mucosa. Both antigens were isolated biochemically and one was highly purified. One antigen, renal tubular epithelium (RTE)-α4, appears to be a lipoprotein, so20w of 28S, and the other a protein, so20w of 9.8S. RTE-α3 is immunogenic but is masked in situ so that direct interaction with humoral antibody does not occur. This antigen does appear in the serum following tubular epithelial injury. Neither of the isolated RTE-specific antigens is the pathogenetic antigen responsible for experimental allergic glomerulonephritis induced with tubular antigen.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>4862890</pmid><doi>10.4049/jimmunol.99.6.1199</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1967-12, Vol.99 (6), p.1199-1210 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antigens - analysis Chromatography, Ion Exchange Epithelium Fluorescent Antibody Technique Glomerulonephritis - immunology Hypersensitivity Immunoelectrophoresis Kidney Tubules - immunology Male Microscopy, Fluorescence Rabbits Rats Ultracentrifugation |
| title | Characterization and Isolation of Specific Renal Tubular Epithelial Antigens |
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