New electromechanical method for the assay of heparin in vitro
A rapid, simple, and accurate in vitro assay for heparin employing a diatomaceous silicate modified plasma and a precision coagulation timer is reported. The method reveals standard deviations of ± 2.5sec. from the average clotting times with a coefficient of variation of about 2 per cent. Sodium he...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 1967-10, Vol.56 (10), p.1304-1308 |
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| container_end_page | 1308 |
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| container_issue | 10 |
| container_start_page | 1304 |
| container_title | Journal of pharmaceutical sciences |
| container_volume | 56 |
| creator | Seip, William F. Carski, Theodore R. Kramer, David N. |
| description | A rapid, simple, and accurate in vitro assay for heparin employing a diatomaceous silicate modified plasma and a precision coagulation timer is reported. The method reveals standard deviations of ± 2.5sec. from the average clotting times with a coefficient of variation of about 2 per cent. Sodium heparin U.S.P., heparin reference standard lot H, and the second international standard for heparin have been successfully assayed by this method. The assays have also revealed the stability of sodium heparin to autoclaving and high energy electron irradiation. |
| doi_str_mv | 10.1002/jps.2600561018 |
| format | Article |
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The method reveals standard deviations of ± 2.5sec. from the average clotting times with a coefficient of variation of about 2 per cent. Sodium heparin U.S.P., heparin reference standard lot H, and the second international standard for heparin have been successfully assayed by this method. The assays have also revealed the stability of sodium heparin to autoclaving and high energy electron irradiation.</description><identifier>ISSN: 0022-3549</identifier><identifier>EISSN: 1520-6017</identifier><identifier>DOI: 10.1002/jps.2600561018</identifier><identifier>PMID: 4293838</identifier><language>eng</language><publisher>Washington: Elsevier Inc</publisher><subject>Animals ; Blood Coagulation ; Electronics ; Heparin - standards ; In Vitro Techniques ; Methods ; Sheep ; Silicon Dioxide ; Time</subject><ispartof>Journal of pharmaceutical sciences, 1967-10, Vol.56 (10), p.1304-1308</ispartof><rights>1967 Wiley-Liss, Inc., A Wiley Company</rights><rights>Copyright © 1967 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4278-2a67f5cee89bf1ecaa1b8b33bc5cc41af0966011679e53bb693e051b9f691a9d3</citedby><cites>FETCH-LOGICAL-c4278-2a67f5cee89bf1ecaa1b8b33bc5cc41af0966011679e53bb693e051b9f691a9d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjps.2600561018$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjps.2600561018$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4293838$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seip, William F.</creatorcontrib><creatorcontrib>Carski, Theodore R.</creatorcontrib><creatorcontrib>Kramer, David N.</creatorcontrib><title>New electromechanical method for the assay of heparin in vitro</title><title>Journal of pharmaceutical sciences</title><addtitle>J. Pharm. Sci</addtitle><description>A rapid, simple, and accurate in vitro assay for heparin employing a diatomaceous silicate modified plasma and a precision coagulation timer is reported. The method reveals standard deviations of ± 2.5sec. from the average clotting times with a coefficient of variation of about 2 per cent. Sodium heparin U.S.P., heparin reference standard lot H, and the second international standard for heparin have been successfully assayed by this method. The assays have also revealed the stability of sodium heparin to autoclaving and high energy electron irradiation.</description><subject>Animals</subject><subject>Blood Coagulation</subject><subject>Electronics</subject><subject>Heparin - standards</subject><subject>In Vitro Techniques</subject><subject>Methods</subject><subject>Sheep</subject><subject>Silicon Dioxide</subject><subject>Time</subject><issn>0022-3549</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1967</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFLwzAYhoMoc06v3oSevHUmTZM2F0FEpzKm4NyOIU2_ssx2nUm3uX9vpGPiQYRADt_zvuR7gtA5wX2CcXQ1X7p-xDFmnGCSHqAuYREOOSbJIep6IAopi8UxOnFujjHmmLEO6sSRoClNu-h6BJsAStCNrSvQM7UwWpVBBc2szoOitkEzg0A5p7ZBXQQzWCprFoE_a-Mjp-ioUKWDs93dQ2_3d-Pbh3D4PHi8vRmGOo6SNIwUTwqmAVKRFQS0UiRLM0ozzbSOiSqw4P7JhCcCGM0yLihgRjJRcEGUyGkPXba9S1t_rMA1sjJOQ1mqBdQrJ9OY-YUI9WC_BbWtnbNQyKU1lbJbSbD8Fia9MPkjzAcuds2rrIJ8j-8M-blo5xtTwvafNvn08vqrO2yzxjXwuc8q-y55QhMmp6OB5HwyoeNpLCeeT1sevMq1ASudNrDQkBvrf0jmtflrjS-Gb5i8</recordid><startdate>196710</startdate><enddate>196710</enddate><creator>Seip, William F.</creator><creator>Carski, Theodore R.</creator><creator>Kramer, David N.</creator><general>Elsevier Inc</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196710</creationdate><title>New electromechanical method for the assay of heparin in vitro</title><author>Seip, William F. ; Carski, Theodore R. ; Kramer, David N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4278-2a67f5cee89bf1ecaa1b8b33bc5cc41af0966011679e53bb693e051b9f691a9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1967</creationdate><topic>Animals</topic><topic>Blood Coagulation</topic><topic>Electronics</topic><topic>Heparin - standards</topic><topic>In Vitro Techniques</topic><topic>Methods</topic><topic>Sheep</topic><topic>Silicon Dioxide</topic><topic>Time</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seip, William F.</creatorcontrib><creatorcontrib>Carski, Theodore R.</creatorcontrib><creatorcontrib>Kramer, David N.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seip, William F.</au><au>Carski, Theodore R.</au><au>Kramer, David N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New electromechanical method for the assay of heparin in vitro</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><addtitle>J. Pharm. Sci</addtitle><date>1967-10</date><risdate>1967</risdate><volume>56</volume><issue>10</issue><spage>1304</spage><epage>1308</epage><pages>1304-1308</pages><issn>0022-3549</issn><eissn>1520-6017</eissn><abstract>A rapid, simple, and accurate in vitro assay for heparin employing a diatomaceous silicate modified plasma and a precision coagulation timer is reported. The method reveals standard deviations of ± 2.5sec. from the average clotting times with a coefficient of variation of about 2 per cent. Sodium heparin U.S.P., heparin reference standard lot H, and the second international standard for heparin have been successfully assayed by this method. The assays have also revealed the stability of sodium heparin to autoclaving and high energy electron irradiation.</abstract><cop>Washington</cop><pub>Elsevier Inc</pub><pmid>4293838</pmid><doi>10.1002/jps.2600561018</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of pharmaceutical sciences, 1967-10, Vol.56 (10), p.1304-1308 |
| issn | 0022-3549 1520-6017 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84593813 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Animals Blood Coagulation Electronics Heparin - standards In Vitro Techniques Methods Sheep Silicon Dioxide Time |
| title | New electromechanical method for the assay of heparin in vitro |
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