Arbovirus Replication in Mosquito Cell Lines (Singh) Grown in Monolayer or Suspension Culture
Summary Singh's mosquito cell line derived from larval A. albopictus was grown in spinner cultures with a doubling time of 24-30 hr at 23 or 28°. It was found to be adaptable to various partially defined growth media. The typical chromosome count was 6, with 12 chromosomes appearing in 25% of f...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1970-05, Vol.134 (1), p.356-361 |
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| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 134 |
| creator | Stevens, Thomas M. |
| description | Summary
Singh's mosquito cell line derived from larval A. albopictus was grown in spinner cultures with a doubling time of 24-30 hr at 23 or 28°. It was found to be adaptable to various partially defined growth media. The typical chromosome count was 6, with 12 chromosomes appearing in 25% of figures. Infection of the cells with Sindbis virus or type 2 dengue virus at multiplicities of 1 or 2 pfu/cell and maintenance at 28° yielded viral growth cycles similar in timing to those seen in infected vertebrate cells at 37°. Cells chronically infected with Sindbis virus yielded virus at the rate of 1 pfu/cell/-day 7 weeks after infection. Sindbis virus production by cells exposed to actinomycin-D was unaffected by noncytotoxic doses. CPE due to actinomycin occurred at concentrations similar to those affecting vertebrate cells. |
| doi_str_mv | 10.3181/00379727-134-34793 |
| format | Article |
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Singh's mosquito cell line derived from larval A. albopictus was grown in spinner cultures with a doubling time of 24-30 hr at 23 or 28°. It was found to be adaptable to various partially defined growth media. The typical chromosome count was 6, with 12 chromosomes appearing in 25% of figures. Infection of the cells with Sindbis virus or type 2 dengue virus at multiplicities of 1 or 2 pfu/cell and maintenance at 28° yielded viral growth cycles similar in timing to those seen in infected vertebrate cells at 37°. Cells chronically infected with Sindbis virus yielded virus at the rate of 1 pfu/cell/-day 7 weeks after infection. Sindbis virus production by cells exposed to actinomycin-D was unaffected by noncytotoxic doses. CPE due to actinomycin occurred at concentrations similar to those affecting vertebrate cells.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-134-34793</identifier><identifier>PMID: 5423526</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Aedes ; Animals ; Cell Line ; Chromosomes ; Culture Techniques ; Cytopathogenic Effect, Viral ; Dactinomycin - pharmacology ; Dengue Virus - growth & development ; Depression, Chemical ; Encephalitis Viruses - growth & development ; RNA, Viral - biosynthesis ; Virus Cultivation ; Virus Replication</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1970-05, Vol.134 (1), p.356-361</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-3b34f7c065b54fe7cbe125ea9c933d3fba8af22042dc4193b3468c57cff0ff33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5423526$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stevens, Thomas M.</creatorcontrib><title>Arbovirus Replication in Mosquito Cell Lines (Singh) Grown in Monolayer or Suspension Culture</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
Singh's mosquito cell line derived from larval A. albopictus was grown in spinner cultures with a doubling time of 24-30 hr at 23 or 28°. It was found to be adaptable to various partially defined growth media. The typical chromosome count was 6, with 12 chromosomes appearing in 25% of figures. Infection of the cells with Sindbis virus or type 2 dengue virus at multiplicities of 1 or 2 pfu/cell and maintenance at 28° yielded viral growth cycles similar in timing to those seen in infected vertebrate cells at 37°. Cells chronically infected with Sindbis virus yielded virus at the rate of 1 pfu/cell/-day 7 weeks after infection. Sindbis virus production by cells exposed to actinomycin-D was unaffected by noncytotoxic doses. CPE due to actinomycin occurred at concentrations similar to those affecting vertebrate cells.</description><subject>Aedes</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Chromosomes</subject><subject>Culture Techniques</subject><subject>Cytopathogenic Effect, Viral</subject><subject>Dactinomycin - pharmacology</subject><subject>Dengue Virus - growth & development</subject><subject>Depression, Chemical</subject><subject>Encephalitis Viruses - growth & development</subject><subject>RNA, Viral - biosynthesis</subject><subject>Virus Cultivation</subject><subject>Virus Replication</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gCDkJHpYTTLJfhylaBUqgnqVkE2TGtluarJR_Pfu2urR0xzmeV9mHoSOKbkAWtJLQqCoClZkFHgGvKhgB42pAJFBXlW7aDwA2UDso4MY3wihomD5CI0EZyBYPkYvV6H2Hy6kiB_NunFadc632LX43sf35DqPp6Zp8Ny1JuKzJ9cuX8_xLPjPLdT6Rn2ZgH3ATymuTRuH_DQ1XQrmEO1Z1URztJ0T9Hxz_Ty9zeYPs7vp1TzTAGWXQQ3cFprkohbcmkLXhjJhVKUrgAXYWpXKMkY4W2hOqwHPSy0KbS2xFmCCTje16-Dfk4mdXLmo-7NVa3yKsuSigrzkPcg2oA4-xmCsXAe3UuFLUiIHpfJXqeyVyh-lfehk257qlVn8RbYO-_3lZh_V0sg3n0Lb__pf4zcIyX_U</recordid><startdate>197005</startdate><enddate>197005</enddate><creator>Stevens, Thomas M.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197005</creationdate><title>Arbovirus Replication in Mosquito Cell Lines (Singh) Grown in Monolayer or Suspension Culture</title><author>Stevens, Thomas M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-3b34f7c065b54fe7cbe125ea9c933d3fba8af22042dc4193b3468c57cff0ff33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Aedes</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Chromosomes</topic><topic>Culture Techniques</topic><topic>Cytopathogenic Effect, Viral</topic><topic>Dactinomycin - pharmacology</topic><topic>Dengue Virus - growth & development</topic><topic>Depression, Chemical</topic><topic>Encephalitis Viruses - growth & development</topic><topic>RNA, Viral - biosynthesis</topic><topic>Virus Cultivation</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stevens, Thomas M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stevens, Thomas M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arbovirus Replication in Mosquito Cell Lines (Singh) Grown in Monolayer or Suspension Culture</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1970-05</date><risdate>1970</risdate><volume>134</volume><issue>1</issue><spage>356</spage><epage>361</epage><pages>356-361</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
Singh's mosquito cell line derived from larval A. albopictus was grown in spinner cultures with a doubling time of 24-30 hr at 23 or 28°. It was found to be adaptable to various partially defined growth media. The typical chromosome count was 6, with 12 chromosomes appearing in 25% of figures. Infection of the cells with Sindbis virus or type 2 dengue virus at multiplicities of 1 or 2 pfu/cell and maintenance at 28° yielded viral growth cycles similar in timing to those seen in infected vertebrate cells at 37°. Cells chronically infected with Sindbis virus yielded virus at the rate of 1 pfu/cell/-day 7 weeks after infection. Sindbis virus production by cells exposed to actinomycin-D was unaffected by noncytotoxic doses. CPE due to actinomycin occurred at concentrations similar to those affecting vertebrate cells.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>5423526</pmid><doi>10.3181/00379727-134-34793</doi><tpages>6</tpages></addata></record> |
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| ispartof | Experimental biology and medicine (Maywood, N.J.), 1970-05, Vol.134 (1), p.356-361 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Aedes Animals Cell Line Chromosomes Culture Techniques Cytopathogenic Effect, Viral Dactinomycin - pharmacology Dengue Virus - growth & development Depression, Chemical Encephalitis Viruses - growth & development RNA, Viral - biosynthesis Virus Cultivation Virus Replication |
| title | Arbovirus Replication in Mosquito Cell Lines (Singh) Grown in Monolayer or Suspension Culture |
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