Polykaryocyte induction by Newcastle disease virus propagated on different hosts
Department of Microbiology Emory University, Atlanta, Georgia 30322 U.S.A. Infection of cultured cells with Newcastle disease virus may result in the formation of multinucleated cells or polykaryocytes (Johnson & Scott, 1964). Polykaryocytes have been observed early (1 to 3 hr) after infection o...
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| Veröffentlicht in: | Journal of general virology 1970-04, Vol.7 (1), p.81-82 |
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| container_title | Journal of general virology |
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| creator | Young, N.P Ash, R.J |
| description | Department of Microbiology Emory University, Atlanta, Georgia 30322 U.S.A.
Infection of cultured cells with Newcastle disease virus may result in the formation of multinucleated cells or polykaryocytes (Johnson & Scott, 1964). Polykaryocytes have been observed early (1 to 3 hr) after infection of cells with high multiplicities of Newcastle disease virus (Kohn, 1965). However, strains of Newcastle disease virus have different cell-fusing potencies (Kohn & Fuchs, 1969). We report here differences in the ability of Newcastle disease virus stocks propagated on chicken and human cells to induce early polykaryocyte formation.
HeLa cells (obtained from Dr W. A. Cassel) were grown in Eagle's minimal essential medium (MEM) containing 10% calf serum. The os strain of Newcastle disease virus, assayed on HeLa cells as previously described (Ash & Bubel, 1966), was employed in all experiments. Virus titres were expressed in terms of haemadsorption focal units (h.f.u.)/ml.
Received 30 September 1969;
accepted 25 November 1969. |
| doi_str_mv | 10.1099/0022-1317-7-1-81 |
| format | Article |
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Infection of cultured cells with Newcastle disease virus may result in the formation of multinucleated cells or polykaryocytes (Johnson & Scott, 1964). Polykaryocytes have been observed early (1 to 3 hr) after infection of cells with high multiplicities of Newcastle disease virus (Kohn, 1965). However, strains of Newcastle disease virus have different cell-fusing potencies (Kohn & Fuchs, 1969). We report here differences in the ability of Newcastle disease virus stocks propagated on chicken and human cells to induce early polykaryocyte formation.
HeLa cells (obtained from Dr W. A. Cassel) were grown in Eagle's minimal essential medium (MEM) containing 10% calf serum. The os strain of Newcastle disease virus, assayed on HeLa cells as previously described (Ash & Bubel, 1966), was employed in all experiments. Virus titres were expressed in terms of haemadsorption focal units (h.f.u.)/ml.
Received 30 September 1969;
accepted 25 November 1969.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-7-1-81</identifier><identifier>PMID: 5463176</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>animal science ; Animals ; Cell Line ; Cell Nucleus ; Centrifugation, Density Gradient ; Cesium ; Chick Embryo ; Chlorides ; Culture Techniques ; Cytopathogenic Effect, Viral ; Female ; HeLa Cells ; Humans ; Lipoproteins ; Newcastle disease virus - pathogenicity ; Temperature ; Uterine Cervical Neoplasms ; Viral Proteins ; Virus Cultivation ; zoology</subject><ispartof>Journal of general virology, 1970-04, Vol.7 (1), p.81-82</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-d40459334e88d7615a73031cb18e2ec7728c94142a074087be04b6c4ea4559b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5463176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Young, N.P</creatorcontrib><creatorcontrib>Ash, R.J</creatorcontrib><title>Polykaryocyte induction by Newcastle disease virus propagated on different hosts</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Department of Microbiology Emory University, Atlanta, Georgia 30322 U.S.A.
Infection of cultured cells with Newcastle disease virus may result in the formation of multinucleated cells or polykaryocytes (Johnson & Scott, 1964). Polykaryocytes have been observed early (1 to 3 hr) after infection of cells with high multiplicities of Newcastle disease virus (Kohn, 1965). However, strains of Newcastle disease virus have different cell-fusing potencies (Kohn & Fuchs, 1969). We report here differences in the ability of Newcastle disease virus stocks propagated on chicken and human cells to induce early polykaryocyte formation.
HeLa cells (obtained from Dr W. A. Cassel) were grown in Eagle's minimal essential medium (MEM) containing 10% calf serum. The os strain of Newcastle disease virus, assayed on HeLa cells as previously described (Ash & Bubel, 1966), was employed in all experiments. Virus titres were expressed in terms of haemadsorption focal units (h.f.u.)/ml.
Received 30 September 1969;
accepted 25 November 1969.</description><subject>animal science</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Cell Nucleus</subject><subject>Centrifugation, Density Gradient</subject><subject>Cesium</subject><subject>Chick Embryo</subject><subject>Chlorides</subject><subject>Culture Techniques</subject><subject>Cytopathogenic Effect, Viral</subject><subject>Female</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Lipoproteins</subject><subject>Newcastle disease virus - pathogenicity</subject><subject>Temperature</subject><subject>Uterine Cervical Neoplasms</subject><subject>Viral Proteins</subject><subject>Virus Cultivation</subject><subject>zoology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEFP3DAQha2qCLa0914qcmlvgRnbiZ1jhWiLhAAJerYcZ7Lrko0XOwHtv69Xu9rTSPPevJn5GPuKcInQNFcAnJcoUJWqxFLjB7ZAWVclz-JHtjjKZ-xTSv8AUMpKnbLTSta5Wy_Y42MYti82boPbTlT4sZvd5MNYtNvint6dTdNARecT2UTFm49zKjYxbOzSTtQV2dj5vqdI41SsQprSZ3bS2yHRl0M9Z8-_bp6v_5R3D79vr3_elU40MJWdBFk1QkjSulM1VlYJEOha1MTJKcW1ayRKbkFJ0KolkG3tJFlZVU0rztmPfWw-5nWmNJm1T46GwY4U5mR0TufYQDbC3uhiSClSbzbRr_PDBsHsGJodJLODZJRBozGPfDtkz-2auuPAAVrWv-_1lV-u3n0ks6Rx7fOC1geTGR1zLva-3gZjl9En8_eJAwpADhXXUvwHL8qCvw</recordid><startdate>197004</startdate><enddate>197004</enddate><creator>Young, N.P</creator><creator>Ash, R.J</creator><general>Soc General Microbiol</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197004</creationdate><title>Polykaryocyte induction by Newcastle disease virus propagated on different hosts</title><author>Young, N.P ; Ash, R.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-d40459334e88d7615a73031cb18e2ec7728c94142a074087be04b6c4ea4559b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>animal science</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Cell Nucleus</topic><topic>Centrifugation, Density Gradient</topic><topic>Cesium</topic><topic>Chick Embryo</topic><topic>Chlorides</topic><topic>Culture Techniques</topic><topic>Cytopathogenic Effect, Viral</topic><topic>Female</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Lipoproteins</topic><topic>Newcastle disease virus - pathogenicity</topic><topic>Temperature</topic><topic>Uterine Cervical Neoplasms</topic><topic>Viral Proteins</topic><topic>Virus Cultivation</topic><topic>zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Young, N.P</creatorcontrib><creatorcontrib>Ash, R.J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Young, N.P</au><au>Ash, R.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polykaryocyte induction by Newcastle disease virus propagated on different hosts</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1970-04</date><risdate>1970</risdate><volume>7</volume><issue>1</issue><spage>81</spage><epage>82</epage><pages>81-82</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Department of Microbiology Emory University, Atlanta, Georgia 30322 U.S.A.
Infection of cultured cells with Newcastle disease virus may result in the formation of multinucleated cells or polykaryocytes (Johnson & Scott, 1964). Polykaryocytes have been observed early (1 to 3 hr) after infection of cells with high multiplicities of Newcastle disease virus (Kohn, 1965). However, strains of Newcastle disease virus have different cell-fusing potencies (Kohn & Fuchs, 1969). We report here differences in the ability of Newcastle disease virus stocks propagated on chicken and human cells to induce early polykaryocyte formation.
HeLa cells (obtained from Dr W. A. Cassel) were grown in Eagle's minimal essential medium (MEM) containing 10% calf serum. The os strain of Newcastle disease virus, assayed on HeLa cells as previously described (Ash & Bubel, 1966), was employed in all experiments. Virus titres were expressed in terms of haemadsorption focal units (h.f.u.)/ml.
Received 30 September 1969;
accepted 25 November 1969.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>5463176</pmid><doi>10.1099/0022-1317-7-1-81</doi><tpages>2</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1970-04, Vol.7 (1), p.81-82 |
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| language | eng |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | animal science Animals Cell Line Cell Nucleus Centrifugation, Density Gradient Cesium Chick Embryo Chlorides Culture Techniques Cytopathogenic Effect, Viral Female HeLa Cells Humans Lipoproteins Newcastle disease virus - pathogenicity Temperature Uterine Cervical Neoplasms Viral Proteins Virus Cultivation zoology |
| title | Polykaryocyte induction by Newcastle disease virus propagated on different hosts |
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