The Multiplication of Measles Virus in Human Amnion Cells in vitro
Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands The multiplication of the EDMONSTON strain of measles virus in suspensions of human amnion cells was studied. The first new virus appeared in the cytoplasmic fraction of the cells 17 to 20 hr after infection....
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| Veröffentlicht in: | Journal of general virology 1970-04, Vol.7 (1), p.13-17 |
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| container_title | Journal of general virology |
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| creator | De Jong, J. C Winkler, K. C |
| description | Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands
The multiplication of the EDMONSTON strain of measles virus in suspensions of human amnion cells was studied. The first new virus appeared in the cytoplasmic fraction of the cells 17 to 20 hr after infection. After another 7 hr the virus was liberated into the medium. The multiplication of the virus was not inhibited by actinomycin D as measured by virus growth, virus plaque formation, or change in sensitivity to u.v.-irradiation. The u.v.-irradiation of monolayers before infection did not reduce plaque formation.
Received 25 June 1969;
accepted 13 November 1969. |
| doi_str_mv | 10.1099/0022-1317-7-1-13 |
| format | Article |
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The multiplication of the EDMONSTON strain of measles virus in suspensions of human amnion cells was studied. The first new virus appeared in the cytoplasmic fraction of the cells 17 to 20 hr after infection. After another 7 hr the virus was liberated into the medium. The multiplication of the virus was not inhibited by actinomycin D as measured by virus growth, virus plaque formation, or change in sensitivity to u.v.-irradiation. The u.v.-irradiation of monolayers before infection did not reduce plaque formation.
Received 25 June 1969;
accepted 13 November 1969.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-7-1-13</identifier><identifier>PMID: 5447478</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Amnion - microbiology ; Cell Line ; Cell Nucleus - microbiology ; Culture Techniques ; Cytoplasm - microbiology ; Dactinomycin - pharmacology ; Humans ; Measles virus - drug effects ; Measles virus - growth & development ; Measles virus - isolation & purification ; Measles virus - pathogenicity ; Measles virus - radiation effects ; Spectrophotometry ; Time Factors ; Ultraviolet Rays ; Virus Replication - drug effects ; Virus Replication - radiation effects</subject><ispartof>Journal of general virology, 1970-04, Vol.7 (1), p.13-17</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-ad075dd0ba6fc17be3de52579f7b18af29c984aec1c05ce8cfca1408f478f0f33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5447478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Jong, J. C</creatorcontrib><creatorcontrib>Winkler, K. C</creatorcontrib><title>The Multiplication of Measles Virus in Human Amnion Cells in vitro</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands
The multiplication of the EDMONSTON strain of measles virus in suspensions of human amnion cells was studied. The first new virus appeared in the cytoplasmic fraction of the cells 17 to 20 hr after infection. After another 7 hr the virus was liberated into the medium. The multiplication of the virus was not inhibited by actinomycin D as measured by virus growth, virus plaque formation, or change in sensitivity to u.v.-irradiation. The u.v.-irradiation of monolayers before infection did not reduce plaque formation.
Received 25 June 1969;
accepted 13 November 1969.</description><subject>Amnion - microbiology</subject><subject>Cell Line</subject><subject>Cell Nucleus - microbiology</subject><subject>Culture Techniques</subject><subject>Cytoplasm - microbiology</subject><subject>Dactinomycin - pharmacology</subject><subject>Humans</subject><subject>Measles virus - drug effects</subject><subject>Measles virus - growth & development</subject><subject>Measles virus - isolation & purification</subject><subject>Measles virus - pathogenicity</subject><subject>Measles virus - radiation effects</subject><subject>Spectrophotometry</subject><subject>Time Factors</subject><subject>Ultraviolet Rays</subject><subject>Virus Replication - drug effects</subject><subject>Virus Replication - radiation effects</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UD1PwzAQtRColMLOgpQFtoCd2LEzlgooUiuWwmo5zrk1ykexkyL-PQ6NmO507927dw-ha4LvCc7zB4yTJCYp4TGPSWhO0JTQjMVJAE_R9B8-Rxfef2JMKGV8giaMUk65mKLHzQ6idV91dl9ZrTrbNlFrojUoX4GPPqzrfWSbaNnXqonmdTMQFlBVf9OD7Vx7ic6MqjxcjXWG3p-fNotlvHp7eV3MV7FOs6yLVYk5K0tcqMxowgtIS2AJ47nhBRHKJLnOBVWgicZMg9BGK0KxMMGnwSZNZ-juqLt37VcPvpO19TpYUQ20vZcivCZYggMRH4natd47MHLvbK3cjyRYDrHJIRc55CK5JKEJKzejdl_UUP4vjDkF_PaI7-x2920dyC00tQ0HCtvKg3Wjzi_s0XVT</recordid><startdate>197004</startdate><enddate>197004</enddate><creator>De Jong, J. C</creator><creator>Winkler, K. C</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197004</creationdate><title>The Multiplication of Measles Virus in Human Amnion Cells in vitro</title><author>De Jong, J. C ; Winkler, K. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-ad075dd0ba6fc17be3de52579f7b18af29c984aec1c05ce8cfca1408f478f0f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Amnion - microbiology</topic><topic>Cell Line</topic><topic>Cell Nucleus - microbiology</topic><topic>Culture Techniques</topic><topic>Cytoplasm - microbiology</topic><topic>Dactinomycin - pharmacology</topic><topic>Humans</topic><topic>Measles virus - drug effects</topic><topic>Measles virus - growth & development</topic><topic>Measles virus - isolation & purification</topic><topic>Measles virus - pathogenicity</topic><topic>Measles virus - radiation effects</topic><topic>Spectrophotometry</topic><topic>Time Factors</topic><topic>Ultraviolet Rays</topic><topic>Virus Replication - drug effects</topic><topic>Virus Replication - radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Jong, J. C</creatorcontrib><creatorcontrib>Winkler, K. C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Jong, J. C</au><au>Winkler, K. C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Multiplication of Measles Virus in Human Amnion Cells in vitro</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1970-04</date><risdate>1970</risdate><volume>7</volume><issue>1</issue><spage>13</spage><epage>17</epage><pages>13-17</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands
The multiplication of the EDMONSTON strain of measles virus in suspensions of human amnion cells was studied. The first new virus appeared in the cytoplasmic fraction of the cells 17 to 20 hr after infection. After another 7 hr the virus was liberated into the medium. The multiplication of the virus was not inhibited by actinomycin D as measured by virus growth, virus plaque formation, or change in sensitivity to u.v.-irradiation. The u.v.-irradiation of monolayers before infection did not reduce plaque formation.
Received 25 June 1969;
accepted 13 November 1969.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>5447478</pmid><doi>10.1099/0022-1317-7-1-13</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-1317 |
| ispartof | Journal of general virology, 1970-04, Vol.7 (1), p.13-17 |
| issn | 0022-1317 1465-2099 |
| language | eng |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amnion - microbiology Cell Line Cell Nucleus - microbiology Culture Techniques Cytoplasm - microbiology Dactinomycin - pharmacology Humans Measles virus - drug effects Measles virus - growth & development Measles virus - isolation & purification Measles virus - pathogenicity Measles virus - radiation effects Spectrophotometry Time Factors Ultraviolet Rays Virus Replication - drug effects Virus Replication - radiation effects |
| title | The Multiplication of Measles Virus in Human Amnion Cells in vitro |
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