Antioxidant Activity and Inhibition of Lipid Peroxidation in Germinating Seeds of Transgenic Soybean Expressing OsHGGT
Tocochromanols are potent lipid-soluble antioxidants and essential nutrients for human health. Genetic engineering techniques were used to develop soybeans with enhanced vitamin E levels, including tocotrienols, which are not found in soybean. The gene encoding rice homogentisate geranylgeranyl tran...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2011-01, Vol.59 (2), p.584-591 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Kim, Yul Ho Lee, Yu Young Kim, Yong Ho Choi, Man Soo Jeong, Kwang Ho Lee, Seuk Ki Seo, Min Jung Yun, Hong Tai Lee, Choon Ki Kim, Wook Han Lee, Sang Chul Park, Soon Ki Park, Hyang Mi |
| description | Tocochromanols are potent lipid-soluble antioxidants and essential nutrients for human health. Genetic engineering techniques were used to develop soybeans with enhanced vitamin E levels, including tocotrienols, which are not found in soybean. The gene encoding rice homogentisate geranylgeranyl transferase (HGGT) was overexpressed in soybeans using seed-specific and constitutive promoters. The association between abundance of vitamin E isomers and antioxidant activity was investigated during seed germination. With the exception of β-tocotrienol, all vitamin E isomers were detected in germinating seeds expressing OsHGGT. The antioxidant properties of germinating seed extracts were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals and lipid peroxidation (TBARS). Compared with intact wild-type seeds, transgenic seeds showed increases in radical scavenging of 5.4−17 and 23.2−35.3% in the DPPH and ABTS assays, respectively. Furthermore, the lipid peroxidation levels were 2.0−4.5-fold lower in germinating seeds from transgenic lines than in wild-type seeds. Therefore, it appears that the antioxidant potential of transgenic oil-producing plants such as soybean, sunflower, and corn may be enhanced by overexpressing OsHGGT during seed germination. |
| doi_str_mv | 10.1021/jf104012z |
| format | Article |
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Genetic engineering techniques were used to develop soybeans with enhanced vitamin E levels, including tocotrienols, which are not found in soybean. The gene encoding rice homogentisate geranylgeranyl transferase (HGGT) was overexpressed in soybeans using seed-specific and constitutive promoters. The association between abundance of vitamin E isomers and antioxidant activity was investigated during seed germination. With the exception of β-tocotrienol, all vitamin E isomers were detected in germinating seeds expressing OsHGGT. The antioxidant properties of germinating seed extracts were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals and lipid peroxidation (TBARS). Compared with intact wild-type seeds, transgenic seeds showed increases in radical scavenging of 5.4−17 and 23.2−35.3% in the DPPH and ABTS assays, respectively. Furthermore, the lipid peroxidation levels were 2.0−4.5-fold lower in germinating seeds from transgenic lines than in wild-type seeds. Therefore, it appears that the antioxidant potential of transgenic oil-producing plants such as soybean, sunflower, and corn may be enhanced by overexpressing OsHGGT during seed germination.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf104012z</identifier><identifier>PMID: 21175184</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Alkyl and Aryl Transferases - genetics ; Alkyl and Aryl Transferases - metabolism ; Antioxidants - analysis ; Antioxidants - pharmacology ; Biological and medical sciences ; Cereal and baking product industries ; Chemical Aspects of Biotechnology/Molecular Biology ; Food industries ; Fruit and vegetable industries ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Germination ; Glycine max - chemistry ; Glycine max - genetics ; Glycine max - growth & development ; Glycine max - metabolism ; Lipid Peroxidation - drug effects ; Oryza - enzymology ; Plant Extracts - analysis ; Plant Extracts - pharmacology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - chemistry ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - growth & development ; Plants, Genetically Modified - metabolism ; Seeds - chemistry ; Seeds - genetics ; Seeds - growth & development ; Seeds - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2011-01, Vol.59 (2), p.584-591</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a434t-d9c97f4670388036083b29632ce517ff9bc9a550d50f34ae9f1396b3fa4dcca53</citedby><cites>FETCH-LOGICAL-a434t-d9c97f4670388036083b29632ce517ff9bc9a550d50f34ae9f1396b3fa4dcca53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf104012z$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf104012z$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23783621$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21175184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Yul Ho</creatorcontrib><creatorcontrib>Lee, Yu Young</creatorcontrib><creatorcontrib>Kim, Yong Ho</creatorcontrib><creatorcontrib>Choi, Man Soo</creatorcontrib><creatorcontrib>Jeong, Kwang Ho</creatorcontrib><creatorcontrib>Lee, Seuk Ki</creatorcontrib><creatorcontrib>Seo, Min Jung</creatorcontrib><creatorcontrib>Yun, Hong Tai</creatorcontrib><creatorcontrib>Lee, Choon Ki</creatorcontrib><creatorcontrib>Kim, Wook Han</creatorcontrib><creatorcontrib>Lee, Sang Chul</creatorcontrib><creatorcontrib>Park, Soon Ki</creatorcontrib><creatorcontrib>Park, Hyang Mi</creatorcontrib><title>Antioxidant Activity and Inhibition of Lipid Peroxidation in Germinating Seeds of Transgenic Soybean Expressing OsHGGT</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Tocochromanols are potent lipid-soluble antioxidants and essential nutrients for human health. Genetic engineering techniques were used to develop soybeans with enhanced vitamin E levels, including tocotrienols, which are not found in soybean. The gene encoding rice homogentisate geranylgeranyl transferase (HGGT) was overexpressed in soybeans using seed-specific and constitutive promoters. The association between abundance of vitamin E isomers and antioxidant activity was investigated during seed germination. With the exception of β-tocotrienol, all vitamin E isomers were detected in germinating seeds expressing OsHGGT. The antioxidant properties of germinating seed extracts were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals and lipid peroxidation (TBARS). Compared with intact wild-type seeds, transgenic seeds showed increases in radical scavenging of 5.4−17 and 23.2−35.3% in the DPPH and ABTS assays, respectively. Furthermore, the lipid peroxidation levels were 2.0−4.5-fold lower in germinating seeds from transgenic lines than in wild-type seeds. Therefore, it appears that the antioxidant potential of transgenic oil-producing plants such as soybean, sunflower, and corn may be enhanced by overexpressing OsHGGT during seed germination.</description><subject>Alkyl and Aryl Transferases - genetics</subject><subject>Alkyl and Aryl Transferases - metabolism</subject><subject>Antioxidants - analysis</subject><subject>Antioxidants - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cereal and baking product industries</subject><subject>Chemical Aspects of Biotechnology/Molecular Biology</subject><subject>Food industries</subject><subject>Fruit and vegetable industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Germination</subject><subject>Glycine max - chemistry</subject><subject>Glycine max - genetics</subject><subject>Glycine max - growth & development</subject><subject>Glycine max - metabolism</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Oryza - enzymology</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - chemistry</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - growth & development</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Seeds - chemistry</subject><subject>Seeds - genetics</subject><subject>Seeds - growth & development</subject><subject>Seeds - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0E1P4zAQBmBrtWgpHwf-wK4vCHEIzMR24hwrBAWpEkgt58hx7OKqdbp2iuj--nVpgQsnazyPXo1eQs4QrhByvJ5bBA6Y__tBBihyyASi_EkGkJaZFAUekqMY5wAgRQm_yGGOWAqUfEBeh7533Ztrle_pUPfu1fUbqnxLH_yLa1xaetpZOnYr19InE97t-6_zdGTC0vk0-hmdGNPGLZ0G5ePMeKfppNs0Rnl6-7YKJsYte4z3o9H0hBxYtYjmdP8ek-e72-nNfTZ-HD3cDMeZ4oz3WVvpqrS8KIFJCawAyZq8KliujcDS2qrRlRICWgGWcWUqi6wqGmYVb7VWgh2Ti13uKnR_1yb29dJFbRYL5U23jrXkoiw4L3mSlzupQxdjMLZeBbdUYVMj1NuW68-Wk_29T103S9N-yo9aEzjfAxW1WtjUiHbxy7FSsiLH5P7snFVdrWYhmedJDsgAK85A4leS0rGed-vgU13fnPQfK3qaBg</recordid><startdate>20110126</startdate><enddate>20110126</enddate><creator>Kim, Yul Ho</creator><creator>Lee, Yu Young</creator><creator>Kim, Yong Ho</creator><creator>Choi, Man Soo</creator><creator>Jeong, Kwang Ho</creator><creator>Lee, Seuk Ki</creator><creator>Seo, Min Jung</creator><creator>Yun, Hong Tai</creator><creator>Lee, Choon Ki</creator><creator>Kim, Wook Han</creator><creator>Lee, Sang Chul</creator><creator>Park, Soon Ki</creator><creator>Park, Hyang Mi</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110126</creationdate><title>Antioxidant Activity and Inhibition of Lipid Peroxidation in Germinating Seeds of Transgenic Soybean Expressing OsHGGT</title><author>Kim, Yul Ho ; Lee, Yu Young ; Kim, Yong Ho ; Choi, Man Soo ; Jeong, Kwang Ho ; Lee, Seuk Ki ; Seo, Min Jung ; Yun, Hong Tai ; Lee, Choon Ki ; Kim, Wook Han ; Lee, Sang Chul ; Park, Soon Ki ; Park, Hyang Mi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a434t-d9c97f4670388036083b29632ce517ff9bc9a550d50f34ae9f1396b3fa4dcca53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Alkyl and Aryl Transferases - genetics</topic><topic>Alkyl and Aryl Transferases - metabolism</topic><topic>Antioxidants - analysis</topic><topic>Antioxidants - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cereal and baking product industries</topic><topic>Chemical Aspects of Biotechnology/Molecular Biology</topic><topic>Food industries</topic><topic>Fruit and vegetable industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Germination</topic><topic>Glycine max - chemistry</topic><topic>Glycine max - genetics</topic><topic>Glycine max - growth & development</topic><topic>Glycine max - metabolism</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Oryza - enzymology</topic><topic>Plant Extracts - analysis</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified - chemistry</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - growth & development</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Seeds - chemistry</topic><topic>Seeds - genetics</topic><topic>Seeds - growth & development</topic><topic>Seeds - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Yul Ho</creatorcontrib><creatorcontrib>Lee, Yu Young</creatorcontrib><creatorcontrib>Kim, Yong Ho</creatorcontrib><creatorcontrib>Choi, Man Soo</creatorcontrib><creatorcontrib>Jeong, Kwang Ho</creatorcontrib><creatorcontrib>Lee, Seuk Ki</creatorcontrib><creatorcontrib>Seo, Min Jung</creatorcontrib><creatorcontrib>Yun, Hong Tai</creatorcontrib><creatorcontrib>Lee, Choon Ki</creatorcontrib><creatorcontrib>Kim, Wook Han</creatorcontrib><creatorcontrib>Lee, Sang Chul</creatorcontrib><creatorcontrib>Park, Soon Ki</creatorcontrib><creatorcontrib>Park, Hyang Mi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Yul Ho</au><au>Lee, Yu Young</au><au>Kim, Yong Ho</au><au>Choi, Man Soo</au><au>Jeong, Kwang Ho</au><au>Lee, Seuk Ki</au><au>Seo, Min Jung</au><au>Yun, Hong Tai</au><au>Lee, Choon Ki</au><au>Kim, Wook Han</au><au>Lee, Sang Chul</au><au>Park, Soon Ki</au><au>Park, Hyang Mi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant Activity and Inhibition of Lipid Peroxidation in Germinating Seeds of Transgenic Soybean Expressing OsHGGT</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2011-01-26</date><risdate>2011</risdate><volume>59</volume><issue>2</issue><spage>584</spage><epage>591</epage><pages>584-591</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Tocochromanols are potent lipid-soluble antioxidants and essential nutrients for human health. Genetic engineering techniques were used to develop soybeans with enhanced vitamin E levels, including tocotrienols, which are not found in soybean. The gene encoding rice homogentisate geranylgeranyl transferase (HGGT) was overexpressed in soybeans using seed-specific and constitutive promoters. The association between abundance of vitamin E isomers and antioxidant activity was investigated during seed germination. With the exception of β-tocotrienol, all vitamin E isomers were detected in germinating seeds expressing OsHGGT. The antioxidant properties of germinating seed extracts were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals and lipid peroxidation (TBARS). Compared with intact wild-type seeds, transgenic seeds showed increases in radical scavenging of 5.4−17 and 23.2−35.3% in the DPPH and ABTS assays, respectively. Furthermore, the lipid peroxidation levels were 2.0−4.5-fold lower in germinating seeds from transgenic lines than in wild-type seeds. Therefore, it appears that the antioxidant potential of transgenic oil-producing plants such as soybean, sunflower, and corn may be enhanced by overexpressing OsHGGT during seed germination.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>21175184</pmid><doi>10.1021/jf104012z</doi><tpages>8</tpages></addata></record> |
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| subjects | Alkyl and Aryl Transferases - genetics Alkyl and Aryl Transferases - metabolism Antioxidants - analysis Antioxidants - pharmacology Biological and medical sciences Cereal and baking product industries Chemical Aspects of Biotechnology/Molecular Biology Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology Gene Expression Germination Glycine max - chemistry Glycine max - genetics Glycine max - growth & development Glycine max - metabolism Lipid Peroxidation - drug effects Oryza - enzymology Plant Extracts - analysis Plant Extracts - pharmacology Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - chemistry Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Plants, Genetically Modified - metabolism Seeds - chemistry Seeds - genetics Seeds - growth & development Seeds - metabolism |
| title | Antioxidant Activity and Inhibition of Lipid Peroxidation in Germinating Seeds of Transgenic Soybean Expressing OsHGGT |
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