Effect of Dopamine on Viability of BHK-21 Cells
We studied the effects of dopamine added to culture medium on survival of floating or adherent BHK-21 cells differing by organization of actin cytoskeleton. The viability of floating cells more drastically decreased with increasing dopamine concentration and duration of exposure than that of adheren...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2010-09, Vol.149 (3), p.359-363 |
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| creator | Moshkov, D. A Abramova, M. B Shubina, V. S Lavrovskaya, V. P Pavlik, L. L Lezhnev, E. I |
| description | We studied the effects of dopamine added to culture medium on survival of floating or adherent BHK-21 cells differing by organization of actin cytoskeleton. The viability of floating cells more drastically decreased with increasing dopamine concentration and duration of exposure than that of adherent cells. The cells worse adhered to the substrate and formed a monolayer. The formed monolayer degrades, cell borders become blurred, cells, polygonal in the control, are rounded. Preliminary blockade of dopamine receptors with haloperidol, inessential for cell survival and morphology, does not prevent the destructive effect of dopamine on the cells. Ultrastructural study revealed increased density of filamentous actin threads in deep compartments of cell cytoplasm after dopamine treatment, this increase being more pronounced in cells grown in suspension. Bearing in mind the polymerizing effect of dopamine on globular actin in vitro and the fact that the content of this protein in floating cells is higher than in adherent cells, we can conclude that the decrease in viability of BHK-21 cells is caused by interaction of dopamine with cytoplasmic globular actin. |
| doi_str_mv | 10.1007/s10517-010-0946-8 |
| format | Article |
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A ; Abramova, M. B ; Shubina, V. S ; Lavrovskaya, V. P ; Pavlik, L. L ; Lezhnev, E. I</creator><creatorcontrib>Moshkov, D. A ; Abramova, M. B ; Shubina, V. S ; Lavrovskaya, V. P ; Pavlik, L. L ; Lezhnev, E. I</creatorcontrib><description>We studied the effects of dopamine added to culture medium on survival of floating or adherent BHK-21 cells differing by organization of actin cytoskeleton. The viability of floating cells more drastically decreased with increasing dopamine concentration and duration of exposure than that of adherent cells. The cells worse adhered to the substrate and formed a monolayer. The formed monolayer degrades, cell borders become blurred, cells, polygonal in the control, are rounded. Preliminary blockade of dopamine receptors with haloperidol, inessential for cell survival and morphology, does not prevent the destructive effect of dopamine on the cells. Ultrastructural study revealed increased density of filamentous actin threads in deep compartments of cell cytoplasm after dopamine treatment, this increase being more pronounced in cells grown in suspension. Bearing in mind the polymerizing effect of dopamine on globular actin in vitro and the fact that the content of this protein in floating cells is higher than in adherent cells, we can conclude that the decrease in viability of BHK-21 cells is caused by interaction of dopamine with cytoplasmic globular actin.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-010-0946-8</identifier><identifier>PMID: 21246101</identifier><identifier>CODEN: BEXBAN</identifier><language>eng</language><publisher>Boston: Boston : Springer US</publisher><subject>Actins - metabolism ; Actins - ultrastructure ; anatomy and morphology ; Animals ; BHK-21 cells ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cell Line ; Cell Survival - drug effects ; Cricetinae ; Culture Media - chemistry ; Cytoskeleton - drug effects ; Cytoskeleton - ultrastructure ; dopamine ; Dopamine - pharmacology ; Dopamine receptors ; Haloperidol ; Haloperidol - pharmacology ; Internal Medicine ; Kidney - cytology ; Laboratory Medicine ; Microscopy, Electron ; Monomolecular films ; Morphology and Pathomorphology ; mortality ; Muscle proteins ; Pathology ; Phenols ; Receptors, Dopamine - metabolism ; Time Factors</subject><ispartof>Bulletin of experimental biology and medicine, 2010-09, Vol.149 (3), p.359-363</ispartof><rights>Springer Science+Business Media, Inc. 2010</rights><rights>COPYRIGHT 2010 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-2fb7c833a60feea60950ed29a08ccd8b9b0278b26aa7e9b761dcb7bde18991d3</citedby><cites>FETCH-LOGICAL-c492t-2fb7c833a60feea60950ed29a08ccd8b9b0278b26aa7e9b761dcb7bde18991d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-010-0946-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-010-0946-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21246101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moshkov, D. A</creatorcontrib><creatorcontrib>Abramova, M. B</creatorcontrib><creatorcontrib>Shubina, V. S</creatorcontrib><creatorcontrib>Lavrovskaya, V. P</creatorcontrib><creatorcontrib>Pavlik, L. L</creatorcontrib><creatorcontrib>Lezhnev, E. I</creatorcontrib><title>Effect of Dopamine on Viability of BHK-21 Cells</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>We studied the effects of dopamine added to culture medium on survival of floating or adherent BHK-21 cells differing by organization of actin cytoskeleton. The viability of floating cells more drastically decreased with increasing dopamine concentration and duration of exposure than that of adherent cells. The cells worse adhered to the substrate and formed a monolayer. The formed monolayer degrades, cell borders become blurred, cells, polygonal in the control, are rounded. Preliminary blockade of dopamine receptors with haloperidol, inessential for cell survival and morphology, does not prevent the destructive effect of dopamine on the cells. Ultrastructural study revealed increased density of filamentous actin threads in deep compartments of cell cytoplasm after dopamine treatment, this increase being more pronounced in cells grown in suspension. Bearing in mind the polymerizing effect of dopamine on globular actin in vitro and the fact that the content of this protein in floating cells is higher than in adherent cells, we can conclude that the decrease in viability of BHK-21 cells is caused by interaction of dopamine with cytoplasmic globular actin.</description><subject>Actins - metabolism</subject><subject>Actins - ultrastructure</subject><subject>anatomy and morphology</subject><subject>Animals</subject><subject>BHK-21 cells</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Cricetinae</subject><subject>Culture Media - chemistry</subject><subject>Cytoskeleton - drug effects</subject><subject>Cytoskeleton - ultrastructure</subject><subject>dopamine</subject><subject>Dopamine - pharmacology</subject><subject>Dopamine receptors</subject><subject>Haloperidol</subject><subject>Haloperidol - pharmacology</subject><subject>Internal Medicine</subject><subject>Kidney - cytology</subject><subject>Laboratory Medicine</subject><subject>Microscopy, Electron</subject><subject>Monomolecular films</subject><subject>Morphology and Pathomorphology</subject><subject>mortality</subject><subject>Muscle proteins</subject><subject>Pathology</subject><subject>Phenols</subject><subject>Receptors, Dopamine - metabolism</subject><subject>Time Factors</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU9vFSEUxYmxsa_VD-BGJ5rYFS0X5vFnWV9ra2ziwuqWAAOvNDPD6zCz6LeXyVRtjTEkEOB3Tu69B6HXQI6BEHGSgaxBYAIEE1VzLJ-hFawFw5JSeI5WpEC4llLuo4Ocb-cr4fAC7VOgNQcCK3RyHoJ3Y5VCdZZ2pou9r1Jf_YjGxjaO9_PHx8svmEK18W2bX6K9YNrsXz2ch-j60_n15hJffb34vDm9wq5WdMQ0WOEkY4aT4H3Z1Zr4hipDpHONtMoSKqSl3BjhlRUcGmeFbTxIpaBhh-hosd0N6W7yedRdzK4UYHqfpqxlvRa8Bi4K-e4v8jZNQ19q06K0DlAmVKD3C7Q1rdexD2kcjJst9SljlJeZUVao439QZTW-iy71PsTy_kTw4ZHgxpt2vMmpncaY-vwUhAV0Q8p58EHvhtiZ4V4D0XOUeolSlyj1HKWWRfPmobHJdr75rfiVXQHoAuTy1W_98Kfz_7m-XUTBJG22Q8z6-zda3AhIyYji7CdXOqx0</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Moshkov, D. 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A</au><au>Abramova, M. B</au><au>Shubina, V. S</au><au>Lavrovskaya, V. P</au><au>Pavlik, L. L</au><au>Lezhnev, E. I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Dopamine on Viability of BHK-21 Cells</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><addtitle>Bull Exp Biol Med</addtitle><date>2010-09-01</date><risdate>2010</risdate><volume>149</volume><issue>3</issue><spage>359</spage><epage>363</epage><pages>359-363</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><coden>BEXBAN</coden><abstract>We studied the effects of dopamine added to culture medium on survival of floating or adherent BHK-21 cells differing by organization of actin cytoskeleton. The viability of floating cells more drastically decreased with increasing dopamine concentration and duration of exposure than that of adherent cells. The cells worse adhered to the substrate and formed a monolayer. The formed monolayer degrades, cell borders become blurred, cells, polygonal in the control, are rounded. Preliminary blockade of dopamine receptors with haloperidol, inessential for cell survival and morphology, does not prevent the destructive effect of dopamine on the cells. Ultrastructural study revealed increased density of filamentous actin threads in deep compartments of cell cytoplasm after dopamine treatment, this increase being more pronounced in cells grown in suspension. Bearing in mind the polymerizing effect of dopamine on globular actin in vitro and the fact that the content of this protein in floating cells is higher than in adherent cells, we can conclude that the decrease in viability of BHK-21 cells is caused by interaction of dopamine with cytoplasmic globular actin.</abstract><cop>Boston</cop><pub>Boston : Springer US</pub><pmid>21246101</pmid><doi>10.1007/s10517-010-0946-8</doi><tpages>5</tpages></addata></record> |
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| subjects | Actins - metabolism Actins - ultrastructure anatomy and morphology Animals BHK-21 cells Biomedical and Life Sciences Biomedicine Cell Biology Cell Line Cell Survival - drug effects Cricetinae Culture Media - chemistry Cytoskeleton - drug effects Cytoskeleton - ultrastructure dopamine Dopamine - pharmacology Dopamine receptors Haloperidol Haloperidol - pharmacology Internal Medicine Kidney - cytology Laboratory Medicine Microscopy, Electron Monomolecular films Morphology and Pathomorphology mortality Muscle proteins Pathology Phenols Receptors, Dopamine - metabolism Time Factors |
| title | Effect of Dopamine on Viability of BHK-21 Cells |
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