Diagnostic Specificity of Sucrose Hemolysis Test for Paroxysmal Nocturnal Hemoglobinuria
The diagnostic specificity of the various modifications of the sucrose hemolysis test for PNH was examined in detail. In whole blood screening tests the greatest specificity was achieved using citrated or oxalated blood and room temperature incubation (23°). Defibrinated whole blood should not be us...
Gespeichert in:
| Veröffentlicht in: | Blood 1970-04, Vol.35 (4), p.462-475 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 475 |
|---|---|
| container_issue | 4 |
| container_start_page | 462 |
| container_title | Blood |
| container_volume | 35 |
| creator | Hartmann, Robert C. Jenkins, David E. Arnold, Anita B. |
| description | The diagnostic specificity of the various modifications of the sucrose hemolysis test for PNH was examined in detail. In whole blood screening tests the greatest specificity was achieved using citrated or oxalated blood and room temperature incubation (23°). Defibrinated whole blood should not be used since "false positive" hemolysis may occur in blood disorders other than PNH. Mechanisms were suggested for this phenomenon. The validity of the confirmatory sucrose hemolysis test employing normal serum was further reported. Because of the clear, colorless character of serum-sucrose mixtures, an insignificant degree of hemolysis (i.e., less than 5%) is more readily visible than in other PNH hemolytic tests employing undiluted serum. Definitive instructions and criteria for interpretation were given for both the whole blood screening test and the confirmatory sucrose hemolysis test. |
| doi_str_mv | 10.1182/blood.V35.4.462.462 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_84569526</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006497120816759</els_id><sourcerecordid>84569526</sourcerecordid><originalsourceid>FETCH-LOGICAL-c399t-249f535cf71488e096aa4b06ff179d59b33cb5ab52c0d609b70c58ffa341ce3b3</originalsourceid><addsrcrecordid>eNp9kE9LxDAQxYMo67r6CUToyVtr0iRtc_Ag658VFhV2FW8hSZMl0jZr0or99rbu4tHDMAPvzWPmB8A5gglCRXolK-fK5A3ThCQkS8c6AFNE0yKGMIWHYAohzGLCcnQMTkL4gBARnNIJmFBCMM7YFLzfWrFpXGitilZbrayxyrZ95Ey06pR3QUcLXbuqDzZEax3ayDgfvQjvvvtQiyp6cqrtfDNMo29TOWmbzltxCo6MqII-2_cZeL2_W88X8fL54XF-s4wVZqyNU8IMxVSZHJGi0JBlQhAJM2NQzkrKJMZKUiFpqmCZQSZzqGhhjMAEKY0lnoHLXe7Wu89uOJDXNihdVaLRrgu8IDRjNM0GI94Zx6-C14Zvva2F7zmCfOTJf3nygScnfGA51rB1sY_vZK3Lv509wEG_3ul6-PHLas-DsrpRurReq5aXzv6b_wNXgYjt</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>84569526</pqid></control><display><type>article</type><title>Diagnostic Specificity of Sucrose Hemolysis Test for Paroxysmal Nocturnal Hemoglobinuria</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Hartmann, Robert C. ; Jenkins, David E. ; Arnold, Anita B.</creator><creatorcontrib>Hartmann, Robert C. ; Jenkins, David E. ; Arnold, Anita B.</creatorcontrib><description>The diagnostic specificity of the various modifications of the sucrose hemolysis test for PNH was examined in detail. In whole blood screening tests the greatest specificity was achieved using citrated or oxalated blood and room temperature incubation (23°). Defibrinated whole blood should not be used since "false positive" hemolysis may occur in blood disorders other than PNH. Mechanisms were suggested for this phenomenon. The validity of the confirmatory sucrose hemolysis test employing normal serum was further reported. Because of the clear, colorless character of serum-sucrose mixtures, an insignificant degree of hemolysis (i.e., less than 5%) is more readily visible than in other PNH hemolytic tests employing undiluted serum. Definitive instructions and criteria for interpretation were given for both the whole blood screening test and the confirmatory sucrose hemolysis test.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V35.4.462.462</identifier><identifier>PMID: 5443369</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>ABO Blood-Group System ; Blood Transfusion ; Citrates ; Dialysis ; Erythrocytes ; Hematocrit ; Hemoglobinometry ; Hemoglobinuria, Paroxysmal - blood ; Hemoglobinuria, Paroxysmal - diagnosis ; Hemoglobinuria, Paroxysmal - therapy ; Hemolysis ; Humans ; Immune Sera ; Isotonic Solutions ; Oxalates ; Sucrose ; Temperature</subject><ispartof>Blood, 1970-04, Vol.35 (4), p.462-475</ispartof><rights>1970 American Society of Hematology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-249f535cf71488e096aa4b06ff179d59b33cb5ab52c0d609b70c58ffa341ce3b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5443369$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hartmann, Robert C.</creatorcontrib><creatorcontrib>Jenkins, David E.</creatorcontrib><creatorcontrib>Arnold, Anita B.</creatorcontrib><title>Diagnostic Specificity of Sucrose Hemolysis Test for Paroxysmal Nocturnal Hemoglobinuria</title><title>Blood</title><addtitle>Blood</addtitle><description>The diagnostic specificity of the various modifications of the sucrose hemolysis test for PNH was examined in detail. In whole blood screening tests the greatest specificity was achieved using citrated or oxalated blood and room temperature incubation (23°). Defibrinated whole blood should not be used since "false positive" hemolysis may occur in blood disorders other than PNH. Mechanisms were suggested for this phenomenon. The validity of the confirmatory sucrose hemolysis test employing normal serum was further reported. Because of the clear, colorless character of serum-sucrose mixtures, an insignificant degree of hemolysis (i.e., less than 5%) is more readily visible than in other PNH hemolytic tests employing undiluted serum. Definitive instructions and criteria for interpretation were given for both the whole blood screening test and the confirmatory sucrose hemolysis test.</description><subject>ABO Blood-Group System</subject><subject>Blood Transfusion</subject><subject>Citrates</subject><subject>Dialysis</subject><subject>Erythrocytes</subject><subject>Hematocrit</subject><subject>Hemoglobinometry</subject><subject>Hemoglobinuria, Paroxysmal - blood</subject><subject>Hemoglobinuria, Paroxysmal - diagnosis</subject><subject>Hemoglobinuria, Paroxysmal - therapy</subject><subject>Hemolysis</subject><subject>Humans</subject><subject>Immune Sera</subject><subject>Isotonic Solutions</subject><subject>Oxalates</subject><subject>Sucrose</subject><subject>Temperature</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxYMo67r6CUToyVtr0iRtc_Ag658VFhV2FW8hSZMl0jZr0or99rbu4tHDMAPvzWPmB8A5gglCRXolK-fK5A3ThCQkS8c6AFNE0yKGMIWHYAohzGLCcnQMTkL4gBARnNIJmFBCMM7YFLzfWrFpXGitilZbrayxyrZ95Ey06pR3QUcLXbuqDzZEax3ayDgfvQjvvvtQiyp6cqrtfDNMo29TOWmbzltxCo6MqII-2_cZeL2_W88X8fL54XF-s4wVZqyNU8IMxVSZHJGi0JBlQhAJM2NQzkrKJMZKUiFpqmCZQSZzqGhhjMAEKY0lnoHLXe7Wu89uOJDXNihdVaLRrgu8IDRjNM0GI94Zx6-C14Zvva2F7zmCfOTJf3nygScnfGA51rB1sY_vZK3Lv509wEG_3ul6-PHLas-DsrpRurReq5aXzv6b_wNXgYjt</recordid><startdate>197004</startdate><enddate>197004</enddate><creator>Hartmann, Robert C.</creator><creator>Jenkins, David E.</creator><creator>Arnold, Anita B.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197004</creationdate><title>Diagnostic Specificity of Sucrose Hemolysis Test for Paroxysmal Nocturnal Hemoglobinuria</title><author>Hartmann, Robert C. ; Jenkins, David E. ; Arnold, Anita B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-249f535cf71488e096aa4b06ff179d59b33cb5ab52c0d609b70c58ffa341ce3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>ABO Blood-Group System</topic><topic>Blood Transfusion</topic><topic>Citrates</topic><topic>Dialysis</topic><topic>Erythrocytes</topic><topic>Hematocrit</topic><topic>Hemoglobinometry</topic><topic>Hemoglobinuria, Paroxysmal - blood</topic><topic>Hemoglobinuria, Paroxysmal - diagnosis</topic><topic>Hemoglobinuria, Paroxysmal - therapy</topic><topic>Hemolysis</topic><topic>Humans</topic><topic>Immune Sera</topic><topic>Isotonic Solutions</topic><topic>Oxalates</topic><topic>Sucrose</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hartmann, Robert C.</creatorcontrib><creatorcontrib>Jenkins, David E.</creatorcontrib><creatorcontrib>Arnold, Anita B.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hartmann, Robert C.</au><au>Jenkins, David E.</au><au>Arnold, Anita B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnostic Specificity of Sucrose Hemolysis Test for Paroxysmal Nocturnal Hemoglobinuria</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1970-04</date><risdate>1970</risdate><volume>35</volume><issue>4</issue><spage>462</spage><epage>475</epage><pages>462-475</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>The diagnostic specificity of the various modifications of the sucrose hemolysis test for PNH was examined in detail. In whole blood screening tests the greatest specificity was achieved using citrated or oxalated blood and room temperature incubation (23°). Defibrinated whole blood should not be used since "false positive" hemolysis may occur in blood disorders other than PNH. Mechanisms were suggested for this phenomenon. The validity of the confirmatory sucrose hemolysis test employing normal serum was further reported. Because of the clear, colorless character of serum-sucrose mixtures, an insignificant degree of hemolysis (i.e., less than 5%) is more readily visible than in other PNH hemolytic tests employing undiluted serum. Definitive instructions and criteria for interpretation were given for both the whole blood screening test and the confirmatory sucrose hemolysis test.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5443369</pmid><doi>10.1182/blood.V35.4.462.462</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-4971 |
| ispartof | Blood, 1970-04, Vol.35 (4), p.462-475 |
| issn | 0006-4971 1528-0020 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84569526 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ABO Blood-Group System Blood Transfusion Citrates Dialysis Erythrocytes Hematocrit Hemoglobinometry Hemoglobinuria, Paroxysmal - blood Hemoglobinuria, Paroxysmal - diagnosis Hemoglobinuria, Paroxysmal - therapy Hemolysis Humans Immune Sera Isotonic Solutions Oxalates Sucrose Temperature |
| title | Diagnostic Specificity of Sucrose Hemolysis Test for Paroxysmal Nocturnal Hemoglobinuria |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T08%3A04%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Diagnostic%20Specificity%20of%20Sucrose%20Hemolysis%20Test%20for%20Paroxysmal%20Nocturnal%20Hemoglobinuria&rft.jtitle=Blood&rft.au=Hartmann,%20Robert%20C.&rft.date=1970-04&rft.volume=35&rft.issue=4&rft.spage=462&rft.epage=475&rft.pages=462-475&rft.issn=0006-4971&rft.eissn=1528-0020&rft_id=info:doi/10.1182/blood.V35.4.462.462&rft_dat=%3Cproquest_cross%3E84569526%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=84569526&rft_id=info:pmid/5443369&rft_els_id=S0006497120816759&rfr_iscdi=true |