Degradation of glycerophosphatides during storage of saline-washed, saline-suspended red cells at -20 degrees C
When fresh intact red cells were washed and suspended in 0.153 M NaCl and then frozen-stored, the glycerophosphatide levels decreased significantly. Degradation began within 2 wk. Loss of phospholipid was not observed with hemoglobin-free red cell ghosts or plasma stored as long as 2 and 6 months, r...
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Veröffentlicht in: | Journal of lipid research 1967-09, Vol.8 (5), p.518-521 |
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description | When fresh intact red cells were washed and suspended in 0.153 M NaCl and then frozen-stored, the glycerophosphatide levels decreased significantly. Degradation began within 2 wk. Loss of phospholipid was not observed with hemoglobin-free red cell ghosts or plasma stored as long as 2 and 6 months, respectively. |
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Degradation began within 2 wk. Loss of phospholipid was not observed with hemoglobin-free red cell ghosts or plasma stored as long as 2 and 6 months, respectively.</description><identifier>ISSN: 0022-2275</identifier><identifier>PMID: 6049679</identifier><language>eng</language><publisher>United States</publisher><subject>Blood Preservation ; Cholesterol - analysis ; Chromatography, Thin Layer ; Erythrocytes - chemistry ; Freezing ; Hematocrit ; Hemoglobinometry ; Humans ; Phospholipids - analysis ; Phosphorus - analysis ; Sodium Chloride</subject><ispartof>Journal of lipid research, 1967-09, Vol.8 (5), p.518-521</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6049679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ways, P O</creatorcontrib><title>Degradation of glycerophosphatides during storage of saline-washed, saline-suspended red cells at -20 degrees C</title><title>Journal of lipid research</title><addtitle>J Lipid Res</addtitle><description>When fresh intact red cells were washed and suspended in 0.153 M NaCl and then frozen-stored, the glycerophosphatide levels decreased significantly. Degradation began within 2 wk. Loss of phospholipid was not observed with hemoglobin-free red cell ghosts or plasma stored as long as 2 and 6 months, respectively.</description><subject>Blood Preservation</subject><subject>Cholesterol - analysis</subject><subject>Chromatography, Thin Layer</subject><subject>Erythrocytes - chemistry</subject><subject>Freezing</subject><subject>Hematocrit</subject><subject>Hemoglobinometry</subject><subject>Humans</subject><subject>Phospholipids - analysis</subject><subject>Phosphorus - analysis</subject><subject>Sodium Chloride</subject><issn>0022-2275</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1967</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE9Lw0AQxfeg1Fr9CMKePBmYbHc3yVHqnwoFL72HSXaSRpJs3EmQfntTjIfHMI8fb4Z3JdYASkVKJeZG3DJ_AcRa23glVhZ0ZpNsLfwL1QEdjo3vpa9k3Z5LCn44eR5Os-uIpZtC09eSRx-wpgvF2DY9RT_IJ3JP_ytPPFDvyMkwq6S2ZYmjjBRIN1-hOWp3J64rbJnul7kRx7fX424fHT7fP3bPh2hQYMdIp0lqqi0AFA5izCwAxnGiU6WpLKgCwjjLdFGSMWgIEqsckSVX2qpSersRj3-xQ_DfE_GYdw1fPsKe_MR5qo2FLDEz-LCAU9GRy4fQdBjO-VLQ9he88mIB</recordid><startdate>196709</startdate><enddate>196709</enddate><creator>Ways, P O</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>196709</creationdate><title>Degradation of glycerophosphatides during storage of saline-washed, saline-suspended red cells at -20 degrees C</title><author>Ways, P O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p206t-48785f3000bd01a9600a1174824ecbef0ea1994bce55a5e0762dee6edc6ff243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1967</creationdate><topic>Blood Preservation</topic><topic>Cholesterol - analysis</topic><topic>Chromatography, Thin Layer</topic><topic>Erythrocytes - chemistry</topic><topic>Freezing</topic><topic>Hematocrit</topic><topic>Hemoglobinometry</topic><topic>Humans</topic><topic>Phospholipids - analysis</topic><topic>Phosphorus - analysis</topic><topic>Sodium Chloride</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ways, P O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of lipid research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ways, P O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Degradation of glycerophosphatides during storage of saline-washed, saline-suspended red cells at -20 degrees C</atitle><jtitle>Journal of lipid research</jtitle><addtitle>J Lipid Res</addtitle><date>1967-09</date><risdate>1967</risdate><volume>8</volume><issue>5</issue><spage>518</spage><epage>521</epage><pages>518-521</pages><issn>0022-2275</issn><abstract>When fresh intact red cells were washed and suspended in 0.153 M NaCl and then frozen-stored, the glycerophosphatide levels decreased significantly. Degradation began within 2 wk. Loss of phospholipid was not observed with hemoglobin-free red cell ghosts or plasma stored as long as 2 and 6 months, respectively.</abstract><cop>United States</cop><pmid>6049679</pmid><tpages>4</tpages></addata></record> |
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source | American Society for Biochemistry and Molecular Biology; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
subjects | Blood Preservation Cholesterol - analysis Chromatography, Thin Layer Erythrocytes - chemistry Freezing Hematocrit Hemoglobinometry Humans Phospholipids - analysis Phosphorus - analysis Sodium Chloride |
title | Degradation of glycerophosphatides during storage of saline-washed, saline-suspended red cells at -20 degrees C |
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