Vitamin B6 Deficiency in Rats: Utilization of 14C-labeled glutamic acid and sodium 2-ketoglutarate
The effect of a vitamin B6 deficiency on the utilization of 14C-labeled glutamic acid and 2-ketoglutarate in brain and liver tissue was investigated. When glutamate-2-14C was injected the activity in the expired carbon dioxide from vitamin B6-depleted rats was less than that from the control rats. T...
Gespeichert in:
| Veröffentlicht in: | The Journal of nutrition 1967-04, Vol.91 (4), p.555-560 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 560 |
|---|---|
| container_issue | 4 |
| container_start_page | 555 |
| container_title | The Journal of nutrition |
| container_volume | 91 |
| creator | Thiele, V.F. Radke, F.H. |
| description | The effect of a vitamin B6 deficiency on the utilization of 14C-labeled glutamic acid and 2-ketoglutarate in brain and liver tissue was investigated. When glutamate-2-14C was injected the activity in the expired carbon dioxide from vitamin B6-depleted rats was less than that from the control rats. Three fractions were isolated from the brain and liver hydrolysates: 1) all amino acids minus glutamic and aspartic acid, 2) glutamic acid, and 3) aspartic acid. There was no difference in the proportion of 14C found in the 3 fractions isolated from the brains of the deficient group as compared with the control group except in the 14C activity of aspartic acid. In this instance the least 14C activity was noted in the pyridoxine-supplemented group. Liver amino acid fractions from the vitamin B6-deficient rats showed lower levels of 14C activity. Variable levels of 14CO2 were obtained upon injection of 2-ketoglutarate-3,5-14C. In the brain fractions 1 and 3 were significantly lower in the vitamin B6-deprived group than in the vitamin-supplemented group. The 3 fractions from vitamin B6-deficient livers showed lower levels of 14C activity than from the control rats, but the difference was only significant with fraction 3. |
| doi_str_mv | 10.1093/jn/91.4.555 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_84528144</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022316623150346</els_id><sourcerecordid>84528144</sourcerecordid><originalsourceid>FETCH-LOGICAL-c325t-b4f5ef5dae266ea92907f9c8de55e4341068d67fc033a49e71ab2958d5d3ee183</originalsourceid><addsrcrecordid>eNptkEtLAzEURrNQaq2uXAtZuZGpeXfiTusTCoJYtyGT3JHUeegkI9Rf79QWV64u3O_cD-5B6ISSKSWaX6yaC02nYiql3ENjQhjLOFXqAB3GuCKEUKHzERopIriSbIyK15BsHRp8rfANlMEFaNwaD4tnm-IlXqZQhW-bQtvgtsRUzLPKFlCBx29Vvzl12LrgsW08jq0PfY1Z9g6p_Y07m-AI7Ze2inC8mxO0vLt9mT9ki6f7x_nVInOcyZQVopRQSm-BKQVWM01mpXa5BylBcEGJyr2alY5wboWGGbUF0zL30nMAmvMJOtv2fnTtZw8xmTpEB1VlG2j7aHIhWU6FGMDzLei6NsYOSvPRhdp2a0OJ2Vg0q8ZoaoQZLA706a62L2rwf-xO4ZDLbQ7Db18BOhN_JYIPHbhkfBv-7f0Bt7yBHg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>84528144</pqid></control><display><type>article</type><title>Vitamin B6 Deficiency in Rats: Utilization of 14C-labeled glutamic acid and sodium 2-ketoglutarate</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Thiele, V.F. ; Radke, F.H.</creator><creatorcontrib>Thiele, V.F. ; Radke, F.H.</creatorcontrib><description>The effect of a vitamin B6 deficiency on the utilization of 14C-labeled glutamic acid and 2-ketoglutarate in brain and liver tissue was investigated. When glutamate-2-14C was injected the activity in the expired carbon dioxide from vitamin B6-depleted rats was less than that from the control rats. Three fractions were isolated from the brain and liver hydrolysates: 1) all amino acids minus glutamic and aspartic acid, 2) glutamic acid, and 3) aspartic acid. There was no difference in the proportion of 14C found in the 3 fractions isolated from the brains of the deficient group as compared with the control group except in the 14C activity of aspartic acid. In this instance the least 14C activity was noted in the pyridoxine-supplemented group. Liver amino acid fractions from the vitamin B6-deficient rats showed lower levels of 14C activity. Variable levels of 14CO2 were obtained upon injection of 2-ketoglutarate-3,5-14C. In the brain fractions 1 and 3 were significantly lower in the vitamin B6-deprived group than in the vitamin-supplemented group. The 3 fractions from vitamin B6-deficient livers showed lower levels of 14C activity than from the control rats, but the difference was only significant with fraction 3.</description><identifier>ISSN: 0022-3166</identifier><identifier>DOI: 10.1093/jn/91.4.555</identifier><identifier>PMID: 6043652</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acids - metabolism ; Animals ; Aspartic Acid - metabolism ; Body Weight ; Brain - metabolism ; Carbon Dioxide - metabolism ; Carbon Isotopes ; Diet ; Glutamates - metabolism ; Ketoglutaric Acids - metabolism ; Liver - metabolism ; Male ; Organ Size ; Rats ; Vitamin B 6 Deficiency - metabolism</subject><ispartof>The Journal of nutrition, 1967-04, Vol.91 (4), p.555-560</ispartof><rights>1967 American Society for Nutrition.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c325t-b4f5ef5dae266ea92907f9c8de55e4341068d67fc033a49e71ab2958d5d3ee183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6043652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thiele, V.F.</creatorcontrib><creatorcontrib>Radke, F.H.</creatorcontrib><title>Vitamin B6 Deficiency in Rats: Utilization of 14C-labeled glutamic acid and sodium 2-ketoglutarate</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>The effect of a vitamin B6 deficiency on the utilization of 14C-labeled glutamic acid and 2-ketoglutarate in brain and liver tissue was investigated. When glutamate-2-14C was injected the activity in the expired carbon dioxide from vitamin B6-depleted rats was less than that from the control rats. Three fractions were isolated from the brain and liver hydrolysates: 1) all amino acids minus glutamic and aspartic acid, 2) glutamic acid, and 3) aspartic acid. There was no difference in the proportion of 14C found in the 3 fractions isolated from the brains of the deficient group as compared with the control group except in the 14C activity of aspartic acid. In this instance the least 14C activity was noted in the pyridoxine-supplemented group. Liver amino acid fractions from the vitamin B6-deficient rats showed lower levels of 14C activity. Variable levels of 14CO2 were obtained upon injection of 2-ketoglutarate-3,5-14C. In the brain fractions 1 and 3 were significantly lower in the vitamin B6-deprived group than in the vitamin-supplemented group. The 3 fractions from vitamin B6-deficient livers showed lower levels of 14C activity than from the control rats, but the difference was only significant with fraction 3.</description><subject>Amino Acids - metabolism</subject><subject>Animals</subject><subject>Aspartic Acid - metabolism</subject><subject>Body Weight</subject><subject>Brain - metabolism</subject><subject>Carbon Dioxide - metabolism</subject><subject>Carbon Isotopes</subject><subject>Diet</subject><subject>Glutamates - metabolism</subject><subject>Ketoglutaric Acids - metabolism</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Organ Size</subject><subject>Rats</subject><subject>Vitamin B 6 Deficiency - metabolism</subject><issn>0022-3166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1967</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEtLAzEURrNQaq2uXAtZuZGpeXfiTusTCoJYtyGT3JHUeegkI9Rf79QWV64u3O_cD-5B6ISSKSWaX6yaC02nYiql3ENjQhjLOFXqAB3GuCKEUKHzERopIriSbIyK15BsHRp8rfANlMEFaNwaD4tnm-IlXqZQhW-bQtvgtsRUzLPKFlCBx29Vvzl12LrgsW08jq0PfY1Z9g6p_Y07m-AI7Ze2inC8mxO0vLt9mT9ki6f7x_nVInOcyZQVopRQSm-BKQVWM01mpXa5BylBcEGJyr2alY5wboWGGbUF0zL30nMAmvMJOtv2fnTtZw8xmTpEB1VlG2j7aHIhWU6FGMDzLei6NsYOSvPRhdp2a0OJ2Vg0q8ZoaoQZLA706a62L2rwf-xO4ZDLbQ7Db18BOhN_JYIPHbhkfBv-7f0Bt7yBHg</recordid><startdate>196704</startdate><enddate>196704</enddate><creator>Thiele, V.F.</creator><creator>Radke, F.H.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196704</creationdate><title>Vitamin B6 Deficiency in Rats: Utilization of 14C-labeled glutamic acid and sodium 2-ketoglutarate</title><author>Thiele, V.F. ; Radke, F.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-b4f5ef5dae266ea92907f9c8de55e4341068d67fc033a49e71ab2958d5d3ee183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1967</creationdate><topic>Amino Acids - metabolism</topic><topic>Animals</topic><topic>Aspartic Acid - metabolism</topic><topic>Body Weight</topic><topic>Brain - metabolism</topic><topic>Carbon Dioxide - metabolism</topic><topic>Carbon Isotopes</topic><topic>Diet</topic><topic>Glutamates - metabolism</topic><topic>Ketoglutaric Acids - metabolism</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Organ Size</topic><topic>Rats</topic><topic>Vitamin B 6 Deficiency - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thiele, V.F.</creatorcontrib><creatorcontrib>Radke, F.H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thiele, V.F.</au><au>Radke, F.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vitamin B6 Deficiency in Rats: Utilization of 14C-labeled glutamic acid and sodium 2-ketoglutarate</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>1967-04</date><risdate>1967</risdate><volume>91</volume><issue>4</issue><spage>555</spage><epage>560</epage><pages>555-560</pages><issn>0022-3166</issn><abstract>The effect of a vitamin B6 deficiency on the utilization of 14C-labeled glutamic acid and 2-ketoglutarate in brain and liver tissue was investigated. When glutamate-2-14C was injected the activity in the expired carbon dioxide from vitamin B6-depleted rats was less than that from the control rats. Three fractions were isolated from the brain and liver hydrolysates: 1) all amino acids minus glutamic and aspartic acid, 2) glutamic acid, and 3) aspartic acid. There was no difference in the proportion of 14C found in the 3 fractions isolated from the brains of the deficient group as compared with the control group except in the 14C activity of aspartic acid. In this instance the least 14C activity was noted in the pyridoxine-supplemented group. Liver amino acid fractions from the vitamin B6-deficient rats showed lower levels of 14C activity. Variable levels of 14CO2 were obtained upon injection of 2-ketoglutarate-3,5-14C. In the brain fractions 1 and 3 were significantly lower in the vitamin B6-deprived group than in the vitamin-supplemented group. The 3 fractions from vitamin B6-deficient livers showed lower levels of 14C activity than from the control rats, but the difference was only significant with fraction 3.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6043652</pmid><doi>10.1093/jn/91.4.555</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3166 |
| ispartof | The Journal of nutrition, 1967-04, Vol.91 (4), p.555-560 |
| issn | 0022-3166 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84528144 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amino Acids - metabolism Animals Aspartic Acid - metabolism Body Weight Brain - metabolism Carbon Dioxide - metabolism Carbon Isotopes Diet Glutamates - metabolism Ketoglutaric Acids - metabolism Liver - metabolism Male Organ Size Rats Vitamin B 6 Deficiency - metabolism |
| title | Vitamin B6 Deficiency in Rats: Utilization of 14C-labeled glutamic acid and sodium 2-ketoglutarate |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T09%3A18%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Vitamin%20B6%20Deficiency%20in%20Rats:%20Utilization%20of%2014C-labeled%20glutamic%20acid%20and%20sodium%202-ketoglutarate&rft.jtitle=The%20Journal%20of%20nutrition&rft.au=Thiele,%20V.F.&rft.date=1967-04&rft.volume=91&rft.issue=4&rft.spage=555&rft.epage=560&rft.pages=555-560&rft.issn=0022-3166&rft_id=info:doi/10.1093/jn/91.4.555&rft_dat=%3Cproquest_cross%3E84528144%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=84528144&rft_id=info:pmid/6043652&rft_els_id=S0022316623150346&rfr_iscdi=true |