A Method for Determining Deoxyribonucleic Acid Synthetic Time and Mitotic Time of Heart Muscle by Autoradiographic Technique
A new method to estimate the DNA synthetic time and mitotic time of heart muscle of rats from single autoradiographic specimen was described. If the postsynthetic time is negligible, the DNA synthetic time of heart muscle cells is 15-16 hours in 2-day-old, 1- and 2-week-old rats. The mitotic time of...
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| Veröffentlicht in: | The Tohoku Journal of Experimental Medicine 1970, Vol.100(1), pp.15-21 |
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| container_title | The Tohoku Journal of Experimental Medicine |
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| creator | Sasaki, Rikuro Morishita, Toshiaki Yamagata, Shoichi |
| description | A new method to estimate the DNA synthetic time and mitotic time of heart muscle of rats from single autoradiographic specimen was described. If the postsynthetic time is negligible, the DNA synthetic time of heart muscle cells is 15-16 hours in 2-day-old, 1- and 2-week-old rats. The mitotic time of heart muscle cells is 7-11 hours. The DNA synthetic time of interstitial cells is 15-18 hours in 2-day, 1-, 2- and 3-week-old rats. The mitotic time of interstitial cells is 7-9 hours. The DNA synthetic time was also calculated from the estimation of cell number and the results were compared with the above mentioned values. In addition, a method to approach the postsynthetic time was mentioned. |
| doi_str_mv | 10.1620/tjem.100.15 |
| format | Article |
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If the postsynthetic time is negligible, the DNA synthetic time of heart muscle cells is 15-16 hours in 2-day-old, 1- and 2-week-old rats. The mitotic time of heart muscle cells is 7-11 hours. The DNA synthetic time of interstitial cells is 15-18 hours in 2-day, 1-, 2- and 3-week-old rats. The mitotic time of interstitial cells is 7-9 hours. The DNA synthetic time was also calculated from the estimation of cell number and the results were compared with the above mentioned values. 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Exp. Med.</addtitle><description>A new method to estimate the DNA synthetic time and mitotic time of heart muscle of rats from single autoradiographic specimen was described. If the postsynthetic time is negligible, the DNA synthetic time of heart muscle cells is 15-16 hours in 2-day-old, 1- and 2-week-old rats. The mitotic time of heart muscle cells is 7-11 hours. The DNA synthetic time of interstitial cells is 15-18 hours in 2-day, 1-, 2- and 3-week-old rats. The mitotic time of interstitial cells is 7-9 hours. The DNA synthetic time was also calculated from the estimation of cell number and the results were compared with the above mentioned values. In addition, a method to approach the postsynthetic time was mentioned.</description><subject>Age Factors</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Autoradiography</subject><subject>DNA - biosynthesis</subject><subject>Mathematics</subject><subject>Methods</subject><subject>Mitosis</subject><subject>Myocardium - cytology</subject><subject>Myocardium - metabolism</subject><subject>Rats</subject><subject>Thymidine - metabolism</subject><subject>Time Factors</subject><subject>Tritium</subject><issn>0040-8727</issn><issn>1349-3329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UD1PwzAUtBAISmFiRvLEggJ2YifOWEH5kFoxAHPk2C-NqyYutiNRiR-PS0uX53e-e2e_Q-iKkjuap-Q-LKG7oyQifoRGNGNlkmVpeYxGhDCSiCItztC590tCMkaK_BSdckZzzukI_UzwHEJrNW6sw48QwHWmN_0i9vZ740xt-0GtwCg8UUbj900fWggRfpgOsOw1nptgDxe2wS8gXcDzwccxXG_wZAjWSW3swsl1uxWCanvzNcAFOmnkysPl_hyjz6fpx8NLMnt7fn2YzBLF4i8TyAsOohYkF1JQrbK4EKOp0JIJUeuaybTmSlGZi7pUaVNIXTBoYilV1ogmG6Obne_a2fisD1VnvILVSvZgB18JxkmaFmUU3u6EylnvHTTV2plOuk1FSbXNutpmHUFEPKqv97ZD3YE-aPfhRn6645c-yAUc-BiPidn8edEymv75_Vd-4FUrXQV99gsVUZRs</recordid><startdate>1970</startdate><enddate>1970</enddate><creator>Sasaki, Rikuro</creator><creator>Morishita, Toshiaki</creator><creator>Yamagata, Shoichi</creator><general>Tohoku University Medical Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1970</creationdate><title>A Method for Determining Deoxyribonucleic Acid Synthetic Time and Mitotic Time of Heart Muscle by Autoradiographic Technique</title><author>Sasaki, Rikuro ; Morishita, Toshiaki ; Yamagata, Shoichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4655-e675e8b8068a81dc37274128da488bdb4a2b5cc1a68b9c2f7ad74efd749c3f8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Age Factors</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Autoradiography</topic><topic>DNA - biosynthesis</topic><topic>Mathematics</topic><topic>Methods</topic><topic>Mitosis</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>Rats</topic><topic>Thymidine - metabolism</topic><topic>Time Factors</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sasaki, Rikuro</creatorcontrib><creatorcontrib>Morishita, Toshiaki</creatorcontrib><creatorcontrib>Yamagata, Shoichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Tohoku Journal of Experimental Medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sasaki, Rikuro</au><au>Morishita, Toshiaki</au><au>Yamagata, Shoichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Method for Determining Deoxyribonucleic Acid Synthetic Time and Mitotic Time of Heart Muscle by Autoradiographic Technique</atitle><jtitle>The Tohoku Journal of Experimental Medicine</jtitle><addtitle>Tohoku J. Exp. Med.</addtitle><date>1970</date><risdate>1970</risdate><volume>100</volume><issue>1</issue><spage>15</spage><epage>21</epage><pages>15-21</pages><issn>0040-8727</issn><eissn>1349-3329</eissn><abstract>A new method to estimate the DNA synthetic time and mitotic time of heart muscle of rats from single autoradiographic specimen was described. If the postsynthetic time is negligible, the DNA synthetic time of heart muscle cells is 15-16 hours in 2-day-old, 1- and 2-week-old rats. The mitotic time of heart muscle cells is 7-11 hours. The DNA synthetic time of interstitial cells is 15-18 hours in 2-day, 1-, 2- and 3-week-old rats. The mitotic time of interstitial cells is 7-9 hours. The DNA synthetic time was also calculated from the estimation of cell number and the results were compared with the above mentioned values. In addition, a method to approach the postsynthetic time was mentioned.</abstract><cop>Japan</cop><pub>Tohoku University Medical Press</pub><pmid>5416551</pmid><doi>10.1620/tjem.100.15</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Tohoku Journal of Experimental Medicine, 1970, Vol.100(1), pp.15-21 |
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| subjects | Age Factors Animals Animals, Newborn Autoradiography DNA - biosynthesis Mathematics Methods Mitosis Myocardium - cytology Myocardium - metabolism Rats Thymidine - metabolism Time Factors Tritium |
| title | A Method for Determining Deoxyribonucleic Acid Synthetic Time and Mitotic Time of Heart Muscle by Autoradiographic Technique |
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