Effect of organic and inorganic phosphates on the oxygen equilibrium of human erythrocytes
The effect of organic and inorganic phosphates on the functional properties of purified adult human hemoglobin has been investigated. Adenosine triphosphate, guanosine triphosphate, and 2,3-diphosphoglycerate are particularly effective in decreasing the oxygen affinity of hemoglobin and are responsi...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1967-07, Vol.121 (1), p.96-102 |
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| container_title | Archives of biochemistry and biophysics |
| container_volume | 121 |
| creator | Chanutin, Alfred Curnish, Richard R. |
| description | The effect of organic and inorganic phosphates on the functional properties of purified adult human hemoglobin has been investigated. Adenosine triphosphate, guanosine triphosphate, and 2,3-diphosphoglycerate are particularly effective in decreasing the oxygen affinity of hemoglobin and are responsible for a small change in heme-heme interaction. The di- and mononucleotides have a neglibigle effect on the
log p
1
2
and
n values. Hexametaphosphate causes marked changes in both the oxygen affinity and the oxygen equilibrium curve, but tripolyphosphate, tetrametaphosphate, and pyrophosphate are less effective. The oxygen affinity of hemoglobin is decreased in the presence of 2,3-diphosphoglycerate over a wide pH range. At high concentrations of phosphate buffer, diphosphoglycerate has practically no effect on the
log
p1
2
and
n values. The results are discussed in relation to the changes in the oxyhemoglobin dissociation curve of stored blood. |
| doi_str_mv | 10.1016/0003-9861(67)90013-6 |
| format | Article |
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log p
1
2
and
n values. Hexametaphosphate causes marked changes in both the oxygen affinity and the oxygen equilibrium curve, but tripolyphosphate, tetrametaphosphate, and pyrophosphate are less effective. The oxygen affinity of hemoglobin is decreased in the presence of 2,3-diphosphoglycerate over a wide pH range. At high concentrations of phosphate buffer, diphosphoglycerate has practically no effect on the
log
p1
2
and
n values. The results are discussed in relation to the changes in the oxyhemoglobin dissociation curve of stored blood.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(67)90013-6</identifier><identifier>PMID: 6035074</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenine Nucleotides - pharmacology ; Adenosine Triphosphate - pharmacology ; Erythrocytes - metabolism ; Guanine Nucleotides - pharmacology ; Hemoglobins - metabolism ; Humans ; Hydrogen-Ion Concentration ; In Vitro Techniques ; Oxygen - metabolism ; Phosphates - pharmacology ; Propionates - pharmacology ; Spectrophotometry</subject><ispartof>Archives of biochemistry and biophysics, 1967-07, Vol.121 (1), p.96-102</ispartof><rights>1967</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-ad14dc0c92dd49c6d29f2dcaa822897a800226ba35abc59db9dece0bd444a66d3</citedby><cites>FETCH-LOGICAL-c423t-ad14dc0c92dd49c6d29f2dcaa822897a800226ba35abc59db9dece0bd444a66d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003986167900136$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6035074$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chanutin, Alfred</creatorcontrib><creatorcontrib>Curnish, Richard R.</creatorcontrib><title>Effect of organic and inorganic phosphates on the oxygen equilibrium of human erythrocytes</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>The effect of organic and inorganic phosphates on the functional properties of purified adult human hemoglobin has been investigated. Adenosine triphosphate, guanosine triphosphate, and 2,3-diphosphoglycerate are particularly effective in decreasing the oxygen affinity of hemoglobin and are responsible for a small change in heme-heme interaction. The di- and mononucleotides have a neglibigle effect on the
log p
1
2
and
n values. Hexametaphosphate causes marked changes in both the oxygen affinity and the oxygen equilibrium curve, but tripolyphosphate, tetrametaphosphate, and pyrophosphate are less effective. The oxygen affinity of hemoglobin is decreased in the presence of 2,3-diphosphoglycerate over a wide pH range. At high concentrations of phosphate buffer, diphosphoglycerate has practically no effect on the
log
p1
2
and
n values. The results are discussed in relation to the changes in the oxyhemoglobin dissociation curve of stored blood.</description><subject>Adenine Nucleotides - pharmacology</subject><subject>Adenosine Triphosphate - pharmacology</subject><subject>Erythrocytes - metabolism</subject><subject>Guanine Nucleotides - pharmacology</subject><subject>Hemoglobins - metabolism</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>In Vitro Techniques</subject><subject>Oxygen - metabolism</subject><subject>Phosphates - pharmacology</subject><subject>Propionates - pharmacology</subject><subject>Spectrophotometry</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1967</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9PwyAYhonRzPnjP9CkJ6OHKlDKysXELPNHssSLXrwQCl9XTFs2aI3772Vu7uiJwPe87xcehC4IviWY8DuMcZaKgpNrPrkRGJMs5QdoTLDgKc4KdojGe-QYnYTwGRnCOB2hEcdZjidsjD5mVQW6T1yVOL9QndWJ6kxiu7_bsnZhWaseQuK6pK8hcd_rBXQJrAbb2NLbod2k66FV8dGv-9o7vY78GTqqVBPgfHeeovfH2dv0OZ2_Pr1MH-apZjTrU2UIMxprQY1hQnNDRUWNVqqgtBATVWBMKS9VlqtS58KUwoAGXBrGmOLcZKfoatu79G41QOhla4OGplEduCHIguWxosgiyLag9i4ED5Vcetsqv5YEy41SufElN74kn8hfpZLH2OWufyhbMPvQzmGc32_nED_5ZcHLoC10Goz1Ua00zv6_4AehwIc3</recordid><startdate>196707</startdate><enddate>196707</enddate><creator>Chanutin, Alfred</creator><creator>Curnish, Richard R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196707</creationdate><title>Effect of organic and inorganic phosphates on the oxygen equilibrium of human erythrocytes</title><author>Chanutin, Alfred ; Curnish, Richard R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-ad14dc0c92dd49c6d29f2dcaa822897a800226ba35abc59db9dece0bd444a66d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1967</creationdate><topic>Adenine Nucleotides - pharmacology</topic><topic>Adenosine Triphosphate - pharmacology</topic><topic>Erythrocytes - metabolism</topic><topic>Guanine Nucleotides - pharmacology</topic><topic>Hemoglobins - metabolism</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>In Vitro Techniques</topic><topic>Oxygen - metabolism</topic><topic>Phosphates - pharmacology</topic><topic>Propionates - pharmacology</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chanutin, Alfred</creatorcontrib><creatorcontrib>Curnish, Richard R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chanutin, Alfred</au><au>Curnish, Richard R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of organic and inorganic phosphates on the oxygen equilibrium of human erythrocytes</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1967-07</date><risdate>1967</risdate><volume>121</volume><issue>1</issue><spage>96</spage><epage>102</epage><pages>96-102</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>The effect of organic and inorganic phosphates on the functional properties of purified adult human hemoglobin has been investigated. Adenosine triphosphate, guanosine triphosphate, and 2,3-diphosphoglycerate are particularly effective in decreasing the oxygen affinity of hemoglobin and are responsible for a small change in heme-heme interaction. The di- and mononucleotides have a neglibigle effect on the
log p
1
2
and
n values. Hexametaphosphate causes marked changes in both the oxygen affinity and the oxygen equilibrium curve, but tripolyphosphate, tetrametaphosphate, and pyrophosphate are less effective. The oxygen affinity of hemoglobin is decreased in the presence of 2,3-diphosphoglycerate over a wide pH range. At high concentrations of phosphate buffer, diphosphoglycerate has practically no effect on the
log
p1
2
and
n values. The results are discussed in relation to the changes in the oxyhemoglobin dissociation curve of stored blood.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6035074</pmid><doi>10.1016/0003-9861(67)90013-6</doi><tpages>7</tpages></addata></record> |
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| ispartof | Archives of biochemistry and biophysics, 1967-07, Vol.121 (1), p.96-102 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84500283 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenine Nucleotides - pharmacology Adenosine Triphosphate - pharmacology Erythrocytes - metabolism Guanine Nucleotides - pharmacology Hemoglobins - metabolism Humans Hydrogen-Ion Concentration In Vitro Techniques Oxygen - metabolism Phosphates - pharmacology Propionates - pharmacology Spectrophotometry |
| title | Effect of organic and inorganic phosphates on the oxygen equilibrium of human erythrocytes |
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