Purification and Allosteric Properties of a Nicotinamide Adenine Dinucleotide-linked d(-)-Specific Lactate Dehydrogenase from Butyribacterium rettgeri

A procedure is described for the purification of a nicotinamide adenine dinucleotide-linked, d (-)-specific lactate dehydrogenase from extracts of Butyribacterium rettgeri . This procedure yields a 300-fold purified enzyme with a 28% recovery of the activity present in crude, cell-free extracts. The...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	The Journal of biological chemistry 1967-06, Vol.242 (12), p.2917-2924
Hauptverfasser:	Wittenberger, C L, Fulco, J G
Format:	Artikel
Sprache:	eng
Schlagworte:	Butyrates Cellulose Chromatography, Ion Exchange Eubacterium - enzymology Hydrogen-Ion Concentration Keto Acids Kinetics L-Lactate Dehydrogenase - analysis L-Lactate Dehydrogenase - metabolism NAD Pyruvates - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!