Infection of Chick Embryo Fibroblasts With Template Active RNA From Avian Myeloblastosis Virus
Institute of Experimental Biology and Genetics J. íman and L. Vep ek Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague 6 The effect of different doses of viral RNA extracted from avian myeloblastosis virus was tested on RIF-free chick embryo cells. The results...
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| Veröffentlicht in: | Journal of general virology 1970-01, Vol.6 (1), p.163-168 |
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| container_title | Journal of general virology |
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| creator | Hlozanek, I Sovova, Vlasta Riman, J Veprek, L |
| description | Institute of Experimental Biology and Genetics
J. íman and
L. Vep ek
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague 6
The effect of different doses of viral RNA extracted from avian myeloblastosis virus was tested on RIF-free chick embryo cells. The results showed that the number of cells with positive gs-antigen was probably related to the dose of RNA. The specificity of viral RNA action was shown by a fluorescent antibody method and by the use of ribonuclease.
* Present address: Houghton Poultry Research Station, Houghton, Huntingdon, England.
Part of the work reported in this paper was undertaken during the tenure of an Eleanor Roosevelt International Cancer Fellowship of the American Cancer Society awarded by the International Union Against Cancer.
Received 8 August 1969;
accepted 18 September 1969. |
| doi_str_mv | 10.1099/0022-1317-6-1-163 |
| format | Article |
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J. íman and
L. Vep ek
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague 6
The effect of different doses of viral RNA extracted from avian myeloblastosis virus was tested on RIF-free chick embryo cells. The results showed that the number of cells with positive gs-antigen was probably related to the dose of RNA. The specificity of viral RNA action was shown by a fluorescent antibody method and by the use of ribonuclease.
* Present address: Houghton Poultry Research Station, Houghton, Huntingdon, England.
Part of the work reported in this paper was undertaken during the tenure of an Eleanor Roosevelt International Cancer Fellowship of the American Cancer Society awarded by the International Union Against Cancer.
Received 8 August 1969;
accepted 18 September 1969.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-6-1-163</identifier><identifier>PMID: 4313399</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Animals ; Antigens ; Avian Leukosis Virus - immunology ; Cell Line ; Chick Embryo ; Fibroblasts ; Fluorescent Antibody Technique ; Ribonucleases ; RNA, Viral - pharmacology ; Templates, Genetic</subject><ispartof>Journal of general virology, 1970-01, Vol.6 (1), p.163-168</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-46024275749996933e89b81dae9d07e68291687ac86fe608a5f280149d68f0fa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4313399$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hlozanek, I</creatorcontrib><creatorcontrib>Sovova, Vlasta</creatorcontrib><creatorcontrib>Riman, J</creatorcontrib><creatorcontrib>Veprek, L</creatorcontrib><title>Infection of Chick Embryo Fibroblasts With Template Active RNA From Avian Myeloblastosis Virus</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Institute of Experimental Biology and Genetics
J. íman and
L. Vep ek
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague 6
The effect of different doses of viral RNA extracted from avian myeloblastosis virus was tested on RIF-free chick embryo cells. The results showed that the number of cells with positive gs-antigen was probably related to the dose of RNA. The specificity of viral RNA action was shown by a fluorescent antibody method and by the use of ribonuclease.
* Present address: Houghton Poultry Research Station, Houghton, Huntingdon, England.
Part of the work reported in this paper was undertaken during the tenure of an Eleanor Roosevelt International Cancer Fellowship of the American Cancer Society awarded by the International Union Against Cancer.
Received 8 August 1969;
accepted 18 September 1969.</description><subject>Animals</subject><subject>Antigens</subject><subject>Avian Leukosis Virus - immunology</subject><subject>Cell Line</subject><subject>Chick Embryo</subject><subject>Fibroblasts</subject><subject>Fluorescent Antibody Technique</subject><subject>Ribonucleases</subject><subject>RNA, Viral - pharmacology</subject><subject>Templates, Genetic</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1PwjAYxxujQUQ_gAeTnrxN27Xr2iMhoCSoiUG92XTjGVS3FdsNw7d3BNTTc_i_5fkhdEnJDSVK3RISxxFlNI1ERCMq2BHqUy6SKO7UY9T_00_RWQgfhFDOk7SHepxRxpTqo_dpXUDeWFdjV-DRyuafeFxlfuvwxGbeZaUJTcBvtlnhOVTr0jSAh11gA_j5cYgn3lV4uLGmxg9bKPd-F2zAr9a34RydFKYMcHG4A_QyGc9H99Hs6W46Gs6inKWkibggMY_TJOVKKaEYA6kySRcG1IKkIGSsqJCpyaUoQBBpkiKW3TNqIWRBCsMG6Hrfu_buq4XQ6MqGHMrS1ODaoCXnSnIZd0a6N-beheCh0GtvK-O3mhK9Y6p3zPSOmRaa6o5pl7k6lLdZBYu_xAHi__jKLlff1oNeQl3ZbiGzTm-s_y36AblRffs</recordid><startdate>19700101</startdate><enddate>19700101</enddate><creator>Hlozanek, I</creator><creator>Sovova, Vlasta</creator><creator>Riman, J</creator><creator>Veprek, L</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19700101</creationdate><title>Infection of Chick Embryo Fibroblasts With Template Active RNA From Avian Myeloblastosis Virus</title><author>Hlozanek, I ; Sovova, Vlasta ; Riman, J ; Veprek, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-46024275749996933e89b81dae9d07e68291687ac86fe608a5f280149d68f0fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Avian Leukosis Virus - immunology</topic><topic>Cell Line</topic><topic>Chick Embryo</topic><topic>Fibroblasts</topic><topic>Fluorescent Antibody Technique</topic><topic>Ribonucleases</topic><topic>RNA, Viral - pharmacology</topic><topic>Templates, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hlozanek, I</creatorcontrib><creatorcontrib>Sovova, Vlasta</creatorcontrib><creatorcontrib>Riman, J</creatorcontrib><creatorcontrib>Veprek, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hlozanek, I</au><au>Sovova, Vlasta</au><au>Riman, J</au><au>Veprek, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Infection of Chick Embryo Fibroblasts With Template Active RNA From Avian Myeloblastosis Virus</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1970-01-01</date><risdate>1970</risdate><volume>6</volume><issue>1</issue><spage>163</spage><epage>168</epage><pages>163-168</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Institute of Experimental Biology and Genetics
J. íman and
L. Vep ek
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague 6
The effect of different doses of viral RNA extracted from avian myeloblastosis virus was tested on RIF-free chick embryo cells. The results showed that the number of cells with positive gs-antigen was probably related to the dose of RNA. The specificity of viral RNA action was shown by a fluorescent antibody method and by the use of ribonuclease.
* Present address: Houghton Poultry Research Station, Houghton, Huntingdon, England.
Part of the work reported in this paper was undertaken during the tenure of an Eleanor Roosevelt International Cancer Fellowship of the American Cancer Society awarded by the International Union Against Cancer.
Received 8 August 1969;
accepted 18 September 1969.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>4313399</pmid><doi>10.1099/0022-1317-6-1-163</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1970-01, Vol.6 (1), p.163-168 |
| issn | 0022-1317 1465-2099 |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Antigens Avian Leukosis Virus - immunology Cell Line Chick Embryo Fibroblasts Fluorescent Antibody Technique Ribonucleases RNA, Viral - pharmacology Templates, Genetic |
| title | Infection of Chick Embryo Fibroblasts With Template Active RNA From Avian Myeloblastosis Virus |
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