A SIMPLE AND EFFICIENT METHOD OF SEPARATING PERIPHERAL-BLOOD LEUCOCYTES FOR IN-VITRO STUDIES

A SIMPLE AND EFFICIENT METHOD OF SEPARATING PERIPHERAL-BLOOD LEUCOCYTES FOR IN-VITRO STUDIES

A technique for the separation of leucocytes from blood is described which was found to be suitable in man and several laboratory species—namely, mouse, rat, rabbit, guineapig, and sheep. Blood is overlayered on a solution of high density containing an agglutinating agent. Separation occurs by clump...

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Veröffentlicht in:The Lancet (British edition) 1967-01, Vol.289 (7503), p.1304-1305
Hauptverfasser: Hulliger, Lotte, Blazkovec, A.A.
Format: Artikel
Sprache:eng
Schlagworte:
Agglutination
Animals
Antibodies
Blood Sedimentation
Contrast Media
Erythrocytes
Guinea Pigs
Hemagglutination Tests
Leukocytes
Methylcellulose
Mice
Rabbits
Rats
Sheep
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container_issue 7503
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container_title The Lancet (British edition)
container_volume 289
creator Hulliger, Lotte
Blazkovec, A.A.
description A technique for the separation of leucocytes from blood is described which was found to be suitable in man and several laboratory species—namely, mouse, rat, rabbit, guineapig, and sheep. Blood is overlayered on a solution of high density containing an agglutinating agent. Separation occurs by clumping of the erythrocytes at the interphase followed by rapid sedimentation. In-vitro studies showed that leucocytes separated by this technique reacted normally to phytohæmagglutinin (P.H.A.), and had adequate antibody-forming capacity.
doi_str_mv 10.1016/S0140-6736(67)91596-6
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subjects Agglutination
Animals
Antibodies
Blood Sedimentation
Contrast Media
Erythrocytes
Guinea Pigs
Hemagglutination Tests
Leukocytes
Methylcellulose
Mice
Rabbits
Rats
Sheep
title A SIMPLE AND EFFICIENT METHOD OF SEPARATING PERIPHERAL-BLOOD LEUCOCYTES FOR IN-VITRO STUDIES
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