Human M-Proteins with Antibody Activity for Nitrophenyl Ligands

Human M-Proteins with Antibody Activity for Nitrophenyl Ligands

Human sera containing M-proteins were tested for their capacity to bind nitrophenyl haptens. About 1.5% of 580 M-proteins showed significant binding for either DNP-caproate or DNP-lysine. In all active sera, binding was due to the M-protein. By contrast, tests of 319 sera by immunodiffusion against...

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Veröffentlicht in:The Journal of immunology (1950) 1970-01, Vol.104 (1), p.256-259
Hauptverfasser: Terry, W. D, Boyd, M. M, Rea, J. S, Stein, R
Format: Artikel
Sprache:eng
Schlagworte:
Aminocaproates
Antigen-Antibody Reactions
Bence Jones Protein
Blood Proteins
Dinitrophenols
Haptens
Humans
Immunodiffusion
Immunoelectrophoresis
Lysine
Multiple Myeloma - immunology
Tritium
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Zusammenfassung:Human sera containing M-proteins were tested for their capacity to bind nitrophenyl haptens. About 1.5% of 580 M-proteins showed significant binding for either DNP-caproate or DNP-lysine. In all active sera, binding was due to the M-protein. By contrast, tests of 319 sera by immunodiffusion against nitrophenyl-protein conjugates resulted in many false positive and false negative reactions, indicating that this is a less satisfactory method for detecting M-proteins with antibody activity.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.104.1.256