Double-isotope derivative assay of serum iodothyronines: I. Preparation of acetyl derivatives of thyroxine and triiodothyronine
In order to apply the double-isotope derivative method to assay of total serum thyroxine or triiodothyronine, the stable mono- and diacetyl derivatives of these compounds were prepared and examined for use in an assay procedure. The monoacetyl derivatives were found to be best for this purpose since...
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| Veröffentlicht in: | Analytical biochemistry 1970, Vol.33 (1), p.67-75 |
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| container_title | Analytical biochemistry |
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| creator | Hagen, Garrett A. Diuguid, Lincoln I. Kliman, Bernard Stanbury, John B. |
| description | In order to apply the double-isotope derivative method to assay of total serum thyroxine or triiodothyronine, the stable mono- and diacetyl derivatives of these compounds were prepared and examined for use in an assay procedure. The monoacetyl derivatives were found to be best for this purpose since they show greater stability, are more soluble in polar solvents, and are easier to purify by chemical or chromatographic means. Therefore the
N-acetyl-
3H derivatives of thyroxine and triiodothyronine were used to develop an assay procedure. |
| doi_str_mv | 10.1016/0003-2697(70)90440-9 |
| format | Article |
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N-acetyl-
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N-acetyl-
3H derivatives of thyroxine and triiodothyronine were used to develop an assay procedure.</description><subject>Acetates - chemical synthesis</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Chromatography, Thin Layer</subject><subject>Drug Stability</subject><subject>Hydrogen-Ion Concentration</subject><subject>Infrared Rays</subject><subject>Methods</subject><subject>Nitrites</subject><subject>Solubility</subject><subject>Spectrophotometry</subject><subject>Temperature</subject><subject>Thyroxine</subject><subject>Triiodothyronine</subject><subject>Tritium</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9LAzEQxYMotVa_gUJOooetk2ya3XgQxL-Fgh70HLKbKUa2m5rsFnvyq7tbi-hpYOY3bx5vCDlmMGbA5AUApAmXKjvL4FyBEJCoHTJkoGQCKahdMvxF9slBjO8AjImJHJDBRLCUCzEkX7e-LSpMXPSNXyK1GNzKNG6F1MRo1tTPacTQLqjz1jdv6-BrV2O8pNMxfQ64NKGjfd1zpsRmXf2RiH13s_PZ7VBTW9oE90_okOzNTRXxaFtH5PX-7uXmMZk9PUxvrmcJskw2iVQTyHNmLOQSJrxzr7hVaLhM51yKQnHORMFMCVnJGbclilyKrl9wA1zN0xE5_dFdBv_RYmz0wsUSq8rU6NuocyG6G4J34MkWbIsFWr0MbmHCWm8T6-ZXP3Ps3K4cBh1Lh3WJ1gUsG2290wx0_yHdx6_7-HUGevMhrdJv1jqDww</recordid><startdate>1970</startdate><enddate>1970</enddate><creator>Hagen, Garrett A.</creator><creator>Diuguid, Lincoln I.</creator><creator>Kliman, Bernard</creator><creator>Stanbury, John B.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1970</creationdate><title>Double-isotope derivative assay of serum iodothyronines: I. Preparation of acetyl derivatives of thyroxine and triiodothyronine</title><author>Hagen, Garrett A. ; Diuguid, Lincoln I. ; Kliman, Bernard ; Stanbury, John B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e176t-6950881ad08605241392d9ea263f264b92214b1ac07c212dce48644b9b2a029f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Acetates - chemical synthesis</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Chromatography, Thin Layer</topic><topic>Drug Stability</topic><topic>Hydrogen-Ion Concentration</topic><topic>Infrared Rays</topic><topic>Methods</topic><topic>Nitrites</topic><topic>Solubility</topic><topic>Spectrophotometry</topic><topic>Temperature</topic><topic>Thyroxine</topic><topic>Triiodothyronine</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hagen, Garrett A.</creatorcontrib><creatorcontrib>Diuguid, Lincoln I.</creatorcontrib><creatorcontrib>Kliman, Bernard</creatorcontrib><creatorcontrib>Stanbury, John B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hagen, Garrett A.</au><au>Diuguid, Lincoln I.</au><au>Kliman, Bernard</au><au>Stanbury, John B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Double-isotope derivative assay of serum iodothyronines: I. Preparation of acetyl derivatives of thyroxine and triiodothyronine</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1970</date><risdate>1970</risdate><volume>33</volume><issue>1</issue><spage>67</spage><epage>75</epage><pages>67-75</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>In order to apply the double-isotope derivative method to assay of total serum thyroxine or triiodothyronine, the stable mono- and diacetyl derivatives of these compounds were prepared and examined for use in an assay procedure. The monoacetyl derivatives were found to be best for this purpose since they show greater stability, are more soluble in polar solvents, and are easier to purify by chemical or chromatographic means. Therefore the
N-acetyl-
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| ispartof | Analytical biochemistry, 1970, Vol.33 (1), p.67-75 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetates - chemical synthesis Chemical Phenomena Chemistry, Physical Chromatography, Thin Layer Drug Stability Hydrogen-Ion Concentration Infrared Rays Methods Nitrites Solubility Spectrophotometry Temperature Thyroxine Triiodothyronine Tritium |
| title | Double-isotope derivative assay of serum iodothyronines: I. Preparation of acetyl derivatives of thyroxine and triiodothyronine |
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