Inhibition of Rauscher murine leukemia virus growth in vitro by actinomycin D
The growth of Rauscher murine leukemia virus was studied in vitro by measuring incorporation of uridine- 3H into purified virus. Labeled virus could be recovered from infected cell cultures 2 hours after adding the nucleoside precursor. Virus labeling was prevented by 0.5 μg of actinomycin D per mil...
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Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1967-06, Vol.32 (2), p.175-183 |
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container_title | Virology (New York, N.Y.) |
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creator | Bases, Robert E. King, Alice S. |
description | The growth of Rauscher murine leukemia virus was studied
in vitro by measuring incorporation of uridine-
3H into purified virus. Labeled virus could be recovered from infected cell cultures 2 hours after adding the nucleoside precursor. Virus labeling was prevented by 0.5 μg of actinomycin D per milliliter, but not by inhibitors of cellular DNA synthesis. These results extend the similarities between the murine and avian RNA tumor viruses. 70 S viral RNA was prepared in good yield from purified labeled virus by exposure to 0.5% sodium dodecyl sulfate. |
doi_str_mv | 10.1016/0042-6822(67)90268-1 |
format | Article |
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3H into purified virus. Labeled virus could be recovered from infected cell cultures 2 hours after adding the nucleoside precursor. Virus labeling was prevented by 0.5 μg of actinomycin D per milliliter, but not by inhibitors of cellular DNA synthesis. These results extend the similarities between the murine and avian RNA tumor viruses. 70 S viral RNA was prepared in good yield from purified labeled virus by exposure to 0.5% sodium dodecyl sulfate.</description><subject>Animals</subject><subject>Centrifugation, Density Gradient</subject><subject>Culture Techniques</subject><subject>Cytarabine - pharmacology</subject><subject>Dactinomycin - pharmacology</subject><subject>Detergents - pharmacology</subject><subject>DNA - biosynthesis</subject><subject>Mice</subject><subject>Phosphorus Isotopes</subject><subject>Rauscher Virus - drug effects</subject><subject>Rauscher Virus - growth & development</subject><subject>RNA, Viral - biosynthesis</subject><subject>Thymidine - metabolism</subject><subject>Tritium</subject><subject>Uridine - metabolism</subject><subject>Virus Cultivation</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1967</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotV7eQCEr0cVokskkmY0gXgsVQXQd0syJjXYmNZmp9O2d2tKlq8M5_39uH0InlFxSQsUVIZxlQjF2LuRFSZhQGd1BQ0pKkZGc01003Fr20UFKn6TPpSQDNBCEFUryIXoeNVM_8a0PDQ4Ov5ou2SlEXHfRN4Bn0H1B7Q1e-Ngl_BHDTzvFvunzNgY8WWJjW9-Eemn74t0R2nNmluB4Ew_R-8P92-1TNn55HN3ejDObF7LNWMXBVbJiSjKubAEyt2CdKftTFS0FLXjOqJGCFIUscuIqLp1iBpxzKhdlfojO1nPnMXx3kFpd-2RhNjMNhC5pxbkgkqneyNdGG0NKEZyeR1-buNSU6BVFvUKkV4i0kPqPoqZ92-lmfjepodo2bbD1-vVah_7JhYeok_XQWKh8BNvqKvj_F_wCElKAlg</recordid><startdate>196706</startdate><enddate>196706</enddate><creator>Bases, Robert E.</creator><creator>King, Alice S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196706</creationdate><title>Inhibition of Rauscher murine leukemia virus growth in vitro by actinomycin D</title><author>Bases, Robert E. ; King, Alice S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-2d4efd7d287248c5e73cecfa96828196154321a760557530fd47f82aefff83693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1967</creationdate><topic>Animals</topic><topic>Centrifugation, Density Gradient</topic><topic>Culture Techniques</topic><topic>Cytarabine - pharmacology</topic><topic>Dactinomycin - pharmacology</topic><topic>Detergents - pharmacology</topic><topic>DNA - biosynthesis</topic><topic>Mice</topic><topic>Phosphorus Isotopes</topic><topic>Rauscher Virus - drug effects</topic><topic>Rauscher Virus - growth & development</topic><topic>RNA, Viral - biosynthesis</topic><topic>Thymidine - metabolism</topic><topic>Tritium</topic><topic>Uridine - metabolism</topic><topic>Virus Cultivation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bases, Robert E.</creatorcontrib><creatorcontrib>King, Alice S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bases, Robert E.</au><au>King, Alice S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of Rauscher murine leukemia virus growth in vitro by actinomycin D</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1967-06</date><risdate>1967</risdate><volume>32</volume><issue>2</issue><spage>175</spage><epage>183</epage><pages>175-183</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>The growth of Rauscher murine leukemia virus was studied
in vitro by measuring incorporation of uridine-
3H into purified virus. Labeled virus could be recovered from infected cell cultures 2 hours after adding the nucleoside precursor. Virus labeling was prevented by 0.5 μg of actinomycin D per milliliter, but not by inhibitors of cellular DNA synthesis. These results extend the similarities between the murine and avian RNA tumor viruses. 70 S viral RNA was prepared in good yield from purified labeled virus by exposure to 0.5% sodium dodecyl sulfate.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6025874</pmid><doi>10.1016/0042-6822(67)90268-1</doi><tpages>9</tpages></addata></record> |
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subjects | Animals Centrifugation, Density Gradient Culture Techniques Cytarabine - pharmacology Dactinomycin - pharmacology Detergents - pharmacology DNA - biosynthesis Mice Phosphorus Isotopes Rauscher Virus - drug effects Rauscher Virus - growth & development RNA, Viral - biosynthesis Thymidine - metabolism Tritium Uridine - metabolism Virus Cultivation |
title | Inhibition of Rauscher murine leukemia virus growth in vitro by actinomycin D |
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