Magnetic Resonance Studies of Spin-Labeled Creatine Kinase System and Interaction of Two Paramagnetic Probes

Creatine kinase from rabbit muscle has been specifically labeled with a derivative of a nitroxide free radical on the two essential sulfhydryl groups, one per active site. The conformation in the environment of the enzyme-bound free radical and the change in conformation induced upon binding of the...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1969-09, Vol.64 (1), p.219-226
Hauptverfasser:	Taylor, June S., Leigh, John S., Cohn, Mildred
Format:	Artikel
Sprache:	eng
Schlagworte:	Active sites Adenine Nucleotides Animals Biochemistry Biological Sciences: Biochemistry Creatine Creatine Kinase - analysis Electron Spin Resonance Spectroscopy Enzyme Activation Enzymes Free radicals Ions Magnetic Resonance Spectroscopy Metal ions Metals Nucleotides Protein Binding Protons Rabbits Spin labels Sulfhydryl Compounds
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