Comparison of Physicochemical Properties of Mitochondrial and Nuclear Deoxyribonucleic Acid from Rat Liver Cells
THE discovery of deoxyribonucleic acid (DNA) in mitochondria 1–9 has opened the way for investigations of the long-standing problem of extra-genome cytogenetic determinants. DNA fibres have been found in mitochondria from tissues belonging to various different classes of animals 1 . The principal di...
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| Veröffentlicht in: | Nature (London) 1966-07, Vol.211 (5044), p.79-80 |
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| creator | KAZAKOVA, T. B. MARKOSIAN, K. A. |
| description | THE discovery of deoxyribonucleic acid (DNA) in mitochondria
1–9
has opened the way for investigations of the long-standing problem of extra-genome cytogenetic determinants. DNA fibres have been found in mitochondria from tissues belonging to various different classes of animals
1
. The principal difficulty to be dealt with in the isolation of mitochondrial DNA is caused by the necessity of separating the soluble nuclear DNA, adsorbed on the surface of mitochondria. For this purpose we used a method of purification of mitochondria in ‘Urographin’ density gradient
8
or treatment of the mitochondrial fraction with DNase (150 µg/ml. for 1.5 h at 0° C). Fibres of high-polymer deproteinized DNA were obtained by means of the phenol method after Kirby
10
and Marmur
11
. The average DNA content in liver mitochondria amounted to 0.65 µg/mg protein. |
| doi_str_mv | 10.1038/211079a0 |
| format | Article |
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1–9
has opened the way for investigations of the long-standing problem of extra-genome cytogenetic determinants. DNA fibres have been found in mitochondria from tissues belonging to various different classes of animals
1
. The principal difficulty to be dealt with in the isolation of mitochondrial DNA is caused by the necessity of separating the soluble nuclear DNA, adsorbed on the surface of mitochondria. For this purpose we used a method of purification of mitochondria in ‘Urographin’ density gradient
8
or treatment of the mitochondrial fraction with DNase (150 µg/ml. for 1.5 h at 0° C). Fibres of high-polymer deproteinized DNA were obtained by means of the phenol method after Kirby
10
and Marmur
11
. The average DNA content in liver mitochondria amounted to 0.65 µg/mg protein.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/211079a0</identifier><identifier>PMID: 5967473</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Adenine - analysis ; Animals ; Cell Nucleus ; Chromatography, Paper ; Cytosine - analysis ; DNA - analysis ; Guanine - analysis ; Humanities and Social Sciences ; In Vitro Techniques ; letter ; Liver - cytology ; Mitochondria ; multidisciplinary ; Rats ; Science ; Science (multidisciplinary) ; Thymine - analysis ; Ultracentrifugation</subject><ispartof>Nature (London), 1966-07, Vol.211 (5044), p.79-80</ispartof><rights>Springer Nature Limited 1966</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c312t-7df78e212588d9b668f4dc81e7cf360250b2bf126a59b9b5c495e8a438f3d653</citedby><cites>FETCH-LOGICAL-c312t-7df78e212588d9b668f4dc81e7cf360250b2bf126a59b9b5c495e8a438f3d653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/211079a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/211079a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5967473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KAZAKOVA, T. B.</creatorcontrib><creatorcontrib>MARKOSIAN, K. A.</creatorcontrib><title>Comparison of Physicochemical Properties of Mitochondrial and Nuclear Deoxyribonucleic Acid from Rat Liver Cells</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>THE discovery of deoxyribonucleic acid (DNA) in mitochondria
1–9
has opened the way for investigations of the long-standing problem of extra-genome cytogenetic determinants. DNA fibres have been found in mitochondria from tissues belonging to various different classes of animals
1
. The principal difficulty to be dealt with in the isolation of mitochondrial DNA is caused by the necessity of separating the soluble nuclear DNA, adsorbed on the surface of mitochondria. For this purpose we used a method of purification of mitochondria in ‘Urographin’ density gradient
8
or treatment of the mitochondrial fraction with DNase (150 µg/ml. for 1.5 h at 0° C). Fibres of high-polymer deproteinized DNA were obtained by means of the phenol method after Kirby
10
and Marmur
11
. The average DNA content in liver mitochondria amounted to 0.65 µg/mg protein.</description><subject>Adenine - analysis</subject><subject>Animals</subject><subject>Cell Nucleus</subject><subject>Chromatography, Paper</subject><subject>Cytosine - analysis</subject><subject>DNA - analysis</subject><subject>Guanine - analysis</subject><subject>Humanities and Social Sciences</subject><subject>In Vitro Techniques</subject><subject>letter</subject><subject>Liver - cytology</subject><subject>Mitochondria</subject><subject>multidisciplinary</subject><subject>Rats</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Thymine - analysis</subject><subject>Ultracentrifugation</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1966</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkF1LwzAUhoMoc07BPyDkSvSimo82SS_H_ISpQ3Zf0jRxGW1Tk1bcvzdj0xuvDpzn5eGcF4BzjG4wouKWYIx4LtEBGOOUsyRlgh-CMUJEJEhQdgxOQlgjhDLM0xEYZTnjKadj0M1c00lvg2uhM3Cx2gSrnFrpxipZw4V3nfa91WFLX2wfkWsrbyOTbQVfB1Vr6eGddt8bb0vXbhdWwamyFTTeNfBd9nBuv7SHM13X4RQcGVkHfbafE7B8uF_OnpL52-PzbDpPFMWkT3hluNAEk0yIKi8ZEyatlMCaK0MZIhkqSWkwYTLLy7zMVJpnWsiUCkMrltEJuNxpO-8-Bx36orFBxQNkq90QCpHS6OAkBq92QeVdCF6bovO2kX5TYFRsuy1-u43Ri71zKBtd_QX3ZUZ-veMhkvZD-2LtBt_GL_-7fgBeGIHt</recordid><startdate>19660702</startdate><enddate>19660702</enddate><creator>KAZAKOVA, T. B.</creator><creator>MARKOSIAN, K. A.</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19660702</creationdate><title>Comparison of Physicochemical Properties of Mitochondrial and Nuclear Deoxyribonucleic Acid from Rat Liver Cells</title><author>KAZAKOVA, T. B. ; MARKOSIAN, K. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c312t-7df78e212588d9b668f4dc81e7cf360250b2bf126a59b9b5c495e8a438f3d653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1966</creationdate><topic>Adenine - analysis</topic><topic>Animals</topic><topic>Cell Nucleus</topic><topic>Chromatography, Paper</topic><topic>Cytosine - analysis</topic><topic>DNA - analysis</topic><topic>Guanine - analysis</topic><topic>Humanities and Social Sciences</topic><topic>In Vitro Techniques</topic><topic>letter</topic><topic>Liver - cytology</topic><topic>Mitochondria</topic><topic>multidisciplinary</topic><topic>Rats</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Thymine - analysis</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KAZAKOVA, T. B.</creatorcontrib><creatorcontrib>MARKOSIAN, K. A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KAZAKOVA, T. B.</au><au>MARKOSIAN, K. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Physicochemical Properties of Mitochondrial and Nuclear Deoxyribonucleic Acid from Rat Liver Cells</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1966-07-02</date><risdate>1966</risdate><volume>211</volume><issue>5044</issue><spage>79</spage><epage>80</epage><pages>79-80</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>THE discovery of deoxyribonucleic acid (DNA) in mitochondria
1–9
has opened the way for investigations of the long-standing problem of extra-genome cytogenetic determinants. DNA fibres have been found in mitochondria from tissues belonging to various different classes of animals
1
. The principal difficulty to be dealt with in the isolation of mitochondrial DNA is caused by the necessity of separating the soluble nuclear DNA, adsorbed on the surface of mitochondria. For this purpose we used a method of purification of mitochondria in ‘Urographin’ density gradient
8
or treatment of the mitochondrial fraction with DNase (150 µg/ml. for 1.5 h at 0° C). Fibres of high-polymer deproteinized DNA were obtained by means of the phenol method after Kirby
10
and Marmur
11
. The average DNA content in liver mitochondria amounted to 0.65 µg/mg protein.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>5967473</pmid><doi>10.1038/211079a0</doi><tpages>2</tpages></addata></record> |
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| ispartof | Nature (London), 1966-07, Vol.211 (5044), p.79-80 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | Adenine - analysis Animals Cell Nucleus Chromatography, Paper Cytosine - analysis DNA - analysis Guanine - analysis Humanities and Social Sciences In Vitro Techniques letter Liver - cytology Mitochondria multidisciplinary Rats Science Science (multidisciplinary) Thymine - analysis Ultracentrifugation |
| title | Comparison of Physicochemical Properties of Mitochondrial and Nuclear Deoxyribonucleic Acid from Rat Liver Cells |
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