The occurrence of εN-methyllysine in human urine
1. 1. An unknown ninhydrin-positive compound was detected in human urine when the basic fraction was analysed by ion exchange chromatography and by two-dimensional paper chromatography. The unknown metabolite and ε- N-methyllysine showed similar properties when examined by these techniques. 2. 2. Fr...
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| Veröffentlicht in: | Clinica chimica acta 1969-10, Vol.26 (1), p.147-154 |
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| container_title | Clinica chimica acta |
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| creator | Asatoor, A.M. |
| description | 1.
1. An unknown ninhydrin-positive compound was detected in human urine when the basic fraction was analysed by ion exchange chromatography and by two-dimensional paper chromatography. The unknown metabolite and
ε-
N-methyllysine showed similar properties when examined by these techniques.
2.
2. Fractions from the ion exchange column were analysed by high-voltage electrophoresis and by paper chromatography in three solvent systems. The metabolite was identified as
ε-
N-methyllysine.
3.
3. The mean level in urine from five normal subjects was 39.4μmoles/24h, when estimations were made on hydrolysed urine. Values on unhydrolysed urine were somewhat lower.
4.
4. The excretion of
ε-
N-methyllysine did not diminish appreciably after starvation suggesting that the metabolite is mainly of endogenous origin.
5.
5. It is postulated that urinary
ε-
N-methyllysine originates from breakdown of histories. |
| doi_str_mv | 10.1016/0009-8981(69)90300-3 |
| format | Article |
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1. An unknown ninhydrin-positive compound was detected in human urine when the basic fraction was analysed by ion exchange chromatography and by two-dimensional paper chromatography. The unknown metabolite and
ε-
N-methyllysine showed similar properties when examined by these techniques.
2.
2. Fractions from the ion exchange column were analysed by high-voltage electrophoresis and by paper chromatography in three solvent systems. The metabolite was identified as
ε-
N-methyllysine.
3.
3. The mean level in urine from five normal subjects was 39.4μmoles/24h, when estimations were made on hydrolysed urine. Values on unhydrolysed urine were somewhat lower.
4.
4. The excretion of
ε-
N-methyllysine did not diminish appreciably after starvation suggesting that the metabolite is mainly of endogenous origin.
5.
5. It is postulated that urinary
ε-
N-methyllysine originates from breakdown of histories.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(69)90300-3</identifier><identifier>PMID: 5356590</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acids - analysis ; Amino Acids - urine ; Chromatography, Ion Exchange ; Chromatography, Paper ; Electrophoresis ; Histidine - analysis ; Histones - metabolism ; Humans ; Lysine - urine ; Starvation</subject><ispartof>Clinica chimica acta, 1969-10, Vol.26 (1), p.147-154</ispartof><rights>1969</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-f543db9dc8c5280e57377d83e6c20b2c66aedce05035e10af453129e165203773</citedby><cites>FETCH-LOGICAL-c357t-f543db9dc8c5280e57377d83e6c20b2c66aedce05035e10af453129e165203773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0009-8981(69)90300-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5356590$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Asatoor, A.M.</creatorcontrib><title>The occurrence of εN-methyllysine in human urine</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>1.
1. An unknown ninhydrin-positive compound was detected in human urine when the basic fraction was analysed by ion exchange chromatography and by two-dimensional paper chromatography. The unknown metabolite and
ε-
N-methyllysine showed similar properties when examined by these techniques.
2.
2. Fractions from the ion exchange column were analysed by high-voltage electrophoresis and by paper chromatography in three solvent systems. The metabolite was identified as
ε-
N-methyllysine.
3.
3. The mean level in urine from five normal subjects was 39.4μmoles/24h, when estimations were made on hydrolysed urine. Values on unhydrolysed urine were somewhat lower.
4.
4. The excretion of
ε-
N-methyllysine did not diminish appreciably after starvation suggesting that the metabolite is mainly of endogenous origin.
5.
5. It is postulated that urinary
ε-
N-methyllysine originates from breakdown of histories.</description><subject>Amino Acids - analysis</subject><subject>Amino Acids - urine</subject><subject>Chromatography, Ion Exchange</subject><subject>Chromatography, Paper</subject><subject>Electrophoresis</subject><subject>Histidine - analysis</subject><subject>Histones - metabolism</subject><subject>Humans</subject><subject>Lysine - urine</subject><subject>Starvation</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1969</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1OwzAQhS0EKqVwA5CyQrAIjDOxY2-QUMWfVMGmrK3UmahG-Sl2g9SDcQ3OREKrLlnNPM2bN5qPsXMONxy4vAUAHSut-JXU1xoQIMYDNuYqwxhTnRyy8d5yzE5C-OhlCpKP2EigkELDmPH5kqLW2s57amzfltHP92tc03q5qapNcA1FromWXZ03Ued7ecqOyrwKdLarE_b--DCfPsezt6eX6f0stiiydVyKFIuFLqyyIlFAIsMsKxSStAksEitlToUlEICCOORlKpAnmrgUCfRWnLDLbe7Kt58dhbWpXbBUVXlDbReMSpEjqMGYbo3WtyF4Ks3Kuzr3G8PBDKTMgMEMGIzU5o-UwX7tYpffLWoq9ks7NP38bjun_skvR94E6wZGhfNk16Zo3f8HfgFm03aA</recordid><startdate>196910</startdate><enddate>196910</enddate><creator>Asatoor, A.M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196910</creationdate><title>The occurrence of εN-methyllysine in human urine</title><author>Asatoor, A.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-f543db9dc8c5280e57377d83e6c20b2c66aedce05035e10af453129e165203773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1969</creationdate><topic>Amino Acids - analysis</topic><topic>Amino Acids - urine</topic><topic>Chromatography, Ion Exchange</topic><topic>Chromatography, Paper</topic><topic>Electrophoresis</topic><topic>Histidine - analysis</topic><topic>Histones - metabolism</topic><topic>Humans</topic><topic>Lysine - urine</topic><topic>Starvation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asatoor, A.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asatoor, A.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The occurrence of εN-methyllysine in human urine</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1969-10</date><risdate>1969</risdate><volume>26</volume><issue>1</issue><spage>147</spage><epage>154</epage><pages>147-154</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>1.
1. An unknown ninhydrin-positive compound was detected in human urine when the basic fraction was analysed by ion exchange chromatography and by two-dimensional paper chromatography. The unknown metabolite and
ε-
N-methyllysine showed similar properties when examined by these techniques.
2.
2. Fractions from the ion exchange column were analysed by high-voltage electrophoresis and by paper chromatography in three solvent systems. The metabolite was identified as
ε-
N-methyllysine.
3.
3. The mean level in urine from five normal subjects was 39.4μmoles/24h, when estimations were made on hydrolysed urine. Values on unhydrolysed urine were somewhat lower.
4.
4. The excretion of
ε-
N-methyllysine did not diminish appreciably after starvation suggesting that the metabolite is mainly of endogenous origin.
5.
5. It is postulated that urinary
ε-
N-methyllysine originates from breakdown of histories.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>5356590</pmid><doi>10.1016/0009-8981(69)90300-3</doi><tpages>8</tpages></addata></record> |
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| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acids - analysis Amino Acids - urine Chromatography, Ion Exchange Chromatography, Paper Electrophoresis Histidine - analysis Histones - metabolism Humans Lysine - urine Starvation |
| title | The occurrence of εN-methyllysine in human urine |
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