In vitro metabolic effects of proteolytic digests of bovine growth hormone in adipose tissue
Proteolytic digests of bovine growth hormone (BGH) prepared by controlled tryptic (TBGH) or papain (PBGH) digestion have in vitro metabolic properties which are qualitatively indistinguishable from native BGH preparations when evaluated by their ability to effect glucose- 14C uptake, its oxidation t...
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| Veröffentlicht in: | Metabolism, clinical and experimental clinical and experimental, 1969-11, Vol.18 (11), p.895-900 |
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| container_title | Metabolism, clinical and experimental |
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| creator | Swislocki, Norbert I. |
| description | Proteolytic digests of bovine growth hormone (BGH) prepared by controlled tryptic (TBGH) or papain (PBGH) digestion have in vitro metabolic properties which are qualitatively indistinguishable from native BGH preparations when evaluated by their ability to effect glucose-
14C uptake, its oxidation to CO
2 and incorporation in lipid, glycerol release, and histidine incorporation into protein of adipose tissue obtained from hypophysectomized rats. Chromatographic fractions of TBGH vary as to their relative potencies in stimulating these responses, both with respect to each other and with respect to the total TBGH digest. One fraction of TBGH formed from BGH by the proteolysis of 2–3 peptide bonds, possesses all the qualitative metabolic effects in vitro while exhibiting a moderate loss in potency both in vitro and in growth-promoting activity. Fractions of PBGH had activities similar to the PBGH digest. |
| doi_str_mv | 10.1016/0026-0495(69)90029-8 |
| format | Article |
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14C uptake, its oxidation to CO
2 and incorporation in lipid, glycerol release, and histidine incorporation into protein of adipose tissue obtained from hypophysectomized rats. Chromatographic fractions of TBGH vary as to their relative potencies in stimulating these responses, both with respect to each other and with respect to the total TBGH digest. One fraction of TBGH formed from BGH by the proteolysis of 2–3 peptide bonds, possesses all the qualitative metabolic effects in vitro while exhibiting a moderate loss in potency both in vitro and in growth-promoting activity. Fractions of PBGH had activities similar to the PBGH digest.</description><identifier>ISSN: 0026-0495</identifier><identifier>EISSN: 1532-8600</identifier><identifier>DOI: 10.1016/0026-0495(69)90029-8</identifier><identifier>PMID: 5350554</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipose Tissue - metabolism ; Amino Acids - metabolism ; Animals ; Carbon Isotopes ; Cattle ; Chromatography ; Glucose - metabolism ; Glycerides - metabolism ; Glycerol - metabolism ; Growth Hormone - pharmacology ; Histidine - metabolism ; In Vitro Techniques ; Lipids - biosynthesis ; Male ; Oxidation-Reduction ; Papain - metabolism ; Peptide Hydrolases - metabolism ; Protein Biosynthesis ; Rats ; Trypsin - metabolism</subject><ispartof>Metabolism, clinical and experimental, 1969-11, Vol.18 (11), p.895-900</ispartof><rights>1969</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-3f6e4b05217043e19105cdbcff50ff180d16c928cfa696a1e0485dc5a121e4773</citedby><cites>FETCH-LOGICAL-c272t-3f6e4b05217043e19105cdbcff50ff180d16c928cfa696a1e0485dc5a121e4773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0026-0495(69)90029-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5350554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Swislocki, Norbert I.</creatorcontrib><title>In vitro metabolic effects of proteolytic digests of bovine growth hormone in adipose tissue</title><title>Metabolism, clinical and experimental</title><addtitle>Metabolism</addtitle><description>Proteolytic digests of bovine growth hormone (BGH) prepared by controlled tryptic (TBGH) or papain (PBGH) digestion have in vitro metabolic properties which are qualitatively indistinguishable from native BGH preparations when evaluated by their ability to effect glucose-
14C uptake, its oxidation to CO
2 and incorporation in lipid, glycerol release, and histidine incorporation into protein of adipose tissue obtained from hypophysectomized rats. Chromatographic fractions of TBGH vary as to their relative potencies in stimulating these responses, both with respect to each other and with respect to the total TBGH digest. One fraction of TBGH formed from BGH by the proteolysis of 2–3 peptide bonds, possesses all the qualitative metabolic effects in vitro while exhibiting a moderate loss in potency both in vitro and in growth-promoting activity. Fractions of PBGH had activities similar to the PBGH digest.</description><subject>Adipose Tissue - metabolism</subject><subject>Amino Acids - metabolism</subject><subject>Animals</subject><subject>Carbon Isotopes</subject><subject>Cattle</subject><subject>Chromatography</subject><subject>Glucose - metabolism</subject><subject>Glycerides - metabolism</subject><subject>Glycerol - metabolism</subject><subject>Growth Hormone - pharmacology</subject><subject>Histidine - metabolism</subject><subject>In Vitro Techniques</subject><subject>Lipids - biosynthesis</subject><subject>Male</subject><subject>Oxidation-Reduction</subject><subject>Papain - metabolism</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Protein Biosynthesis</subject><subject>Rats</subject><subject>Trypsin - metabolism</subject><issn>0026-0495</issn><issn>1532-8600</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1969</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo6_rxDxRyEj1UJ22TJhdBxC8QvOhNCG06WSNtsybdlf33Zt3Fo6dh5n3n6yHkhMElAyauAHKRQan4uVAXKmUqkztkyniRZ1IA7JLpn2WfHMT4CQBVJcWETHjBgfNySt6fBrp0Y_C0x7FufOcMRWvRjJF6S-fBj-i71ZjKrZth3JQbv3QD0lnw3-MH_fCh9yl1A61bN_cR6ehiXOAR2bN1F_F4Gw_J2_3d6-1j9vzy8HR785yZvMrHrLACywZ4ziooC2SKATdtY6zlYC2T0DJhVC6NrYUSNUMoJW8Nr1nOsKyq4pCcbeamc78W6Ujdu2iw6-oB_SJqWeaKV7JIxnJjNMHHGNDqeXB9HVaagV5D1Wtiek1MC6V_oWqZ2k638xdNj-1f05Zi0q83OqYnlw6DjsbhYLB1IZHUrXf_L_gB7RiG1g</recordid><startdate>196911</startdate><enddate>196911</enddate><creator>Swislocki, Norbert I.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196911</creationdate><title>In vitro metabolic effects of proteolytic digests of bovine growth hormone in adipose tissue</title><author>Swislocki, Norbert I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-3f6e4b05217043e19105cdbcff50ff180d16c928cfa696a1e0485dc5a121e4773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1969</creationdate><topic>Adipose Tissue - metabolism</topic><topic>Amino Acids - metabolism</topic><topic>Animals</topic><topic>Carbon Isotopes</topic><topic>Cattle</topic><topic>Chromatography</topic><topic>Glucose - metabolism</topic><topic>Glycerides - metabolism</topic><topic>Glycerol - metabolism</topic><topic>Growth Hormone - pharmacology</topic><topic>Histidine - metabolism</topic><topic>In Vitro Techniques</topic><topic>Lipids - biosynthesis</topic><topic>Male</topic><topic>Oxidation-Reduction</topic><topic>Papain - metabolism</topic><topic>Peptide Hydrolases - metabolism</topic><topic>Protein Biosynthesis</topic><topic>Rats</topic><topic>Trypsin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Swislocki, Norbert I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolism, clinical and experimental</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Swislocki, Norbert I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro metabolic effects of proteolytic digests of bovine growth hormone in adipose tissue</atitle><jtitle>Metabolism, clinical and experimental</jtitle><addtitle>Metabolism</addtitle><date>1969-11</date><risdate>1969</risdate><volume>18</volume><issue>11</issue><spage>895</spage><epage>900</epage><pages>895-900</pages><issn>0026-0495</issn><eissn>1532-8600</eissn><abstract>Proteolytic digests of bovine growth hormone (BGH) prepared by controlled tryptic (TBGH) or papain (PBGH) digestion have in vitro metabolic properties which are qualitatively indistinguishable from native BGH preparations when evaluated by their ability to effect glucose-
14C uptake, its oxidation to CO
2 and incorporation in lipid, glycerol release, and histidine incorporation into protein of adipose tissue obtained from hypophysectomized rats. Chromatographic fractions of TBGH vary as to their relative potencies in stimulating these responses, both with respect to each other and with respect to the total TBGH digest. One fraction of TBGH formed from BGH by the proteolysis of 2–3 peptide bonds, possesses all the qualitative metabolic effects in vitro while exhibiting a moderate loss in potency both in vitro and in growth-promoting activity. Fractions of PBGH had activities similar to the PBGH digest.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5350554</pmid><doi>10.1016/0026-0495(69)90029-8</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0026-0495 |
| ispartof | Metabolism, clinical and experimental, 1969-11, Vol.18 (11), p.895-900 |
| issn | 0026-0495 1532-8600 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adipose Tissue - metabolism Amino Acids - metabolism Animals Carbon Isotopes Cattle Chromatography Glucose - metabolism Glycerides - metabolism Glycerol - metabolism Growth Hormone - pharmacology Histidine - metabolism In Vitro Techniques Lipids - biosynthesis Male Oxidation-Reduction Papain - metabolism Peptide Hydrolases - metabolism Protein Biosynthesis Rats Trypsin - metabolism |
| title | In vitro metabolic effects of proteolytic digests of bovine growth hormone in adipose tissue |
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