A Sensitive Method for Assay of Plasma Renin Activity
Renin activity is separated from rabbit plasma by globulin precipitation, dialysis, and DEAE cellulose column chromatography with a Tris chloride buffer system and acid elution, followed by ultrafiltration. The plasma renin extract contains no renin substrate and no angiotensinase activity can be de...
Gespeichert in:
| Veröffentlicht in: | Circulation research 1966-08, Vol.19 (2), p.260-268 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 268 |
|---|---|
| container_issue | 2 |
| container_start_page | 260 |
| container_title | Circulation research |
| container_volume | 19 |
| creator | Lee, Michael R Cook, William F Mckenzie, John K |
| description | Renin activity is separated from rabbit plasma by globulin precipitation, dialysis, and DEAE cellulose column chromatography with a Tris chloride buffer system and acid elution, followed by ultrafiltration. The plasma renin extract contains no renin substrate and no angiotensinase activity can be detected after incubation with angiotensin for 200 hr. Incubation with a known concentration of rabbit renin substrate, similarly free of renin activity and angiotensinase, at pH 6.0 in 0.1 M phosphate buffer forms angiotensin-like activity at a constant rate for up to 200 hr. Recovery and reproducibility are satisfactory. Evidence is presented that the material in the plasma extract behaves similarly to renin and that the incubation product is indistinguishable from angiotensin. |
| doi_str_mv | 10.1161/01.RES.19.2.260 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_84212249</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>84212249</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3785-c324227af874062bdd6c22394f1c3b01031e142339bebe378c2e770f4c6fe5a03</originalsourceid><addsrcrecordid>eNo9kEtPwkAQxzdGg4iePZn05K1lZnb7OhKCj0SjAT1vtu00VFuK3VbCt3cJxMtMZv6Pw0-IW4QAMcIpYLBcrAJMAwoogjMxxpCUr8IYz8UYAFI_lhIuxZW1XwCoJKUjMVKUJJCosQhn3oo3tuqrX_ZeuV-3hVe2nTez1uy9tvTea2Mb4y15U228We58Vb-_FhelqS3fnPZEfD4sPuZP_svb4_N89uLnMk5CN0kRxaZMYgURZUUR5UQyVSXmMgMEiYyKpEwzzthFcuI4hlLlUcmhATkR98febdf-DGx73VQ257o2G24HqxNFSKRSZ5wejXnXWttxqbdd1ZhurxH0AZQG1A6UxlSTdqBc4u5UPWQNF__-Exmnq6O-a-ueO_tdDzvu9JpN3a-1AwsSkHxMowgSd_mHVyj_AJedcKg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>84212249</pqid></control><display><type>article</type><title>A Sensitive Method for Assay of Plasma Renin Activity</title><source>MEDLINE</source><source>American Heart Association Journals</source><source>Journals@Ovid Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Lee, Michael R ; Cook, William F ; Mckenzie, John K</creator><creatorcontrib>Lee, Michael R ; Cook, William F ; Mckenzie, John K</creatorcontrib><description>Renin activity is separated from rabbit plasma by globulin precipitation, dialysis, and DEAE cellulose column chromatography with a Tris chloride buffer system and acid elution, followed by ultrafiltration. The plasma renin extract contains no renin substrate and no angiotensinase activity can be detected after incubation with angiotensin for 200 hr. Incubation with a known concentration of rabbit renin substrate, similarly free of renin activity and angiotensinase, at pH 6.0 in 0.1 M phosphate buffer forms angiotensin-like activity at a constant rate for up to 200 hr. Recovery and reproducibility are satisfactory. Evidence is presented that the material in the plasma extract behaves similarly to renin and that the incubation product is indistinguishable from angiotensin.</description><identifier>ISSN: 0009-7330</identifier><identifier>EISSN: 1524-4571</identifier><identifier>DOI: 10.1161/01.RES.19.2.260</identifier><identifier>PMID: 4288084</identifier><language>eng</language><publisher>United States: American Heart Association, Inc</publisher><subject>Angiotensin II - pharmacology ; Animals ; Biological Assay ; Chromatography ; Endopeptidases - pharmacology ; Peptide Hydrolases - pharmacology ; Rabbits ; Renin - blood</subject><ispartof>Circulation research, 1966-08, Vol.19 (2), p.260-268</ispartof><rights>1966 American Heart Association, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3785-c324227af874062bdd6c22394f1c3b01031e142339bebe378c2e770f4c6fe5a03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3687,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4288084$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Michael R</creatorcontrib><creatorcontrib>Cook, William F</creatorcontrib><creatorcontrib>Mckenzie, John K</creatorcontrib><title>A Sensitive Method for Assay of Plasma Renin Activity</title><title>Circulation research</title><addtitle>Circ Res</addtitle><description>Renin activity is separated from rabbit plasma by globulin precipitation, dialysis, and DEAE cellulose column chromatography with a Tris chloride buffer system and acid elution, followed by ultrafiltration. The plasma renin extract contains no renin substrate and no angiotensinase activity can be detected after incubation with angiotensin for 200 hr. Incubation with a known concentration of rabbit renin substrate, similarly free of renin activity and angiotensinase, at pH 6.0 in 0.1 M phosphate buffer forms angiotensin-like activity at a constant rate for up to 200 hr. Recovery and reproducibility are satisfactory. Evidence is presented that the material in the plasma extract behaves similarly to renin and that the incubation product is indistinguishable from angiotensin.</description><subject>Angiotensin II - pharmacology</subject><subject>Animals</subject><subject>Biological Assay</subject><subject>Chromatography</subject><subject>Endopeptidases - pharmacology</subject><subject>Peptide Hydrolases - pharmacology</subject><subject>Rabbits</subject><subject>Renin - blood</subject><issn>0009-7330</issn><issn>1524-4571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1966</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPwkAQxzdGg4iePZn05K1lZnb7OhKCj0SjAT1vtu00VFuK3VbCt3cJxMtMZv6Pw0-IW4QAMcIpYLBcrAJMAwoogjMxxpCUr8IYz8UYAFI_lhIuxZW1XwCoJKUjMVKUJJCosQhn3oo3tuqrX_ZeuV-3hVe2nTez1uy9tvTea2Mb4y15U228We58Vb-_FhelqS3fnPZEfD4sPuZP_svb4_N89uLnMk5CN0kRxaZMYgURZUUR5UQyVSXmMgMEiYyKpEwzzthFcuI4hlLlUcmhATkR98febdf-DGx73VQ257o2G24HqxNFSKRSZ5wejXnXWttxqbdd1ZhurxH0AZQG1A6UxlSTdqBc4u5UPWQNF__-Exmnq6O-a-ueO_tdDzvu9JpN3a-1AwsSkHxMowgSd_mHVyj_AJedcKg</recordid><startdate>196608</startdate><enddate>196608</enddate><creator>Lee, Michael R</creator><creator>Cook, William F</creator><creator>Mckenzie, John K</creator><general>American Heart Association, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196608</creationdate><title>A Sensitive Method for Assay of Plasma Renin Activity</title><author>Lee, Michael R ; Cook, William F ; Mckenzie, John K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3785-c324227af874062bdd6c22394f1c3b01031e142339bebe378c2e770f4c6fe5a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1966</creationdate><topic>Angiotensin II - pharmacology</topic><topic>Animals</topic><topic>Biological Assay</topic><topic>Chromatography</topic><topic>Endopeptidases - pharmacology</topic><topic>Peptide Hydrolases - pharmacology</topic><topic>Rabbits</topic><topic>Renin - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Michael R</creatorcontrib><creatorcontrib>Cook, William F</creatorcontrib><creatorcontrib>Mckenzie, John K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Michael R</au><au>Cook, William F</au><au>Mckenzie, John K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Sensitive Method for Assay of Plasma Renin Activity</atitle><jtitle>Circulation research</jtitle><addtitle>Circ Res</addtitle><date>1966-08</date><risdate>1966</risdate><volume>19</volume><issue>2</issue><spage>260</spage><epage>268</epage><pages>260-268</pages><issn>0009-7330</issn><eissn>1524-4571</eissn><abstract>Renin activity is separated from rabbit plasma by globulin precipitation, dialysis, and DEAE cellulose column chromatography with a Tris chloride buffer system and acid elution, followed by ultrafiltration. The plasma renin extract contains no renin substrate and no angiotensinase activity can be detected after incubation with angiotensin for 200 hr. Incubation with a known concentration of rabbit renin substrate, similarly free of renin activity and angiotensinase, at pH 6.0 in 0.1 M phosphate buffer forms angiotensin-like activity at a constant rate for up to 200 hr. Recovery and reproducibility are satisfactory. Evidence is presented that the material in the plasma extract behaves similarly to renin and that the incubation product is indistinguishable from angiotensin.</abstract><cop>United States</cop><pub>American Heart Association, Inc</pub><pmid>4288084</pmid><doi>10.1161/01.RES.19.2.260</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-7330 |
| ispartof | Circulation research, 1966-08, Vol.19 (2), p.260-268 |
| issn | 0009-7330 1524-4571 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84212249 |
| source | MEDLINE; American Heart Association Journals; Journals@Ovid Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Angiotensin II - pharmacology Animals Biological Assay Chromatography Endopeptidases - pharmacology Peptide Hydrolases - pharmacology Rabbits Renin - blood |
| title | A Sensitive Method for Assay of Plasma Renin Activity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T19%3A17%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Sensitive%20Method%20for%20Assay%20of%20Plasma%20Renin%20Activity&rft.jtitle=Circulation%20research&rft.au=Lee,%20Michael%20R&rft.date=1966-08&rft.volume=19&rft.issue=2&rft.spage=260&rft.epage=268&rft.pages=260-268&rft.issn=0009-7330&rft.eissn=1524-4571&rft_id=info:doi/10.1161/01.RES.19.2.260&rft_dat=%3Cproquest_cross%3E84212249%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=84212249&rft_id=info:pmid/4288084&rfr_iscdi=true |