The Structure of Extracellular Phosphorylated Galactans from Sporobolomyces Yeasts

Two strains of Sporobolomyces species, NRRL Y-6493 and NRRL Y-6502, produce extracellular, lightly acetylated, phosphorylated galactans when grown on media containing glucose as the carbon source. d -Galactose and d -galactose 6-phosphate were the only carbohydrate components present in strong acid hydrolysates. α- d -Galactose 1-phosphate was isolated after vigorous alkaline hydrolysis. Periodate oxidation analyses of intact and deacetylated polymers indicate that approximately equal proportions of α-1,6- and α-1,3-galactosyl linkages are present. Polymer phosphate occurs exclusively in the form of α- d -galactopyranosyl-1-phospho-6'-galactosyl phosphodiester end groups.