Gas chromatography of sugar phosphates and sugar nucleotides

Gas chromatography of sugar phosphates and sugar nucleotides

Glucose 6-phosphate, various aldose 1-phosphates, and their corresponding sugar nucleotides have been converted into trimethylsilyl ethers and gas chromatographed. Glucose 6-phosphate separates into α- and β-isomers and can be recovered intact on a preparative scale from the column effluent after sp...

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Veröffentlicht in:Analytical biochemistry 1969-05, Vol.29 (2), p.284-292
Hauptverfasser: Eisenberg, Frank, Bolden, Arthur H.
Format: Artikel
Sprache:eng
Schlagworte:
Chromatography, Gas
Ethers
Fructose
Galactose
Glucose
Hexosephosphates - analysis
Temperature
Uracil Nucleotides - analysis
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container_title Analytical biochemistry
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creator Eisenberg, Frank
Bolden, Arthur H.
description Glucose 6-phosphate, various aldose 1-phosphates, and their corresponding sugar nucleotides have been converted into trimethylsilyl ethers and gas chromatographed. Glucose 6-phosphate separates into α- and β-isomers and can be recovered intact on a preparative scale from the column effluent after spontaneous hydrolysis of the ether linkages. The aldose 1-phosphates and sugar nucleotides are not recoverable intact from the column but lose phosphoric acid and nucleoside diphosphate, respectively, to yield the TMS ether of the sugar anhydride. Since the anhydride patterns are reproducibly characteristic, mixtures of nucleotides can be easily separated and their sugar components identified.
doi_str_mv 10.1016/0003-2697(69)90311-X
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subjects Chromatography, Gas
Ethers
Fructose
Galactose
Glucose
Hexosephosphates - analysis
Temperature
Uracil Nucleotides - analysis
title Gas chromatography of sugar phosphates and sugar nucleotides
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