The Selective Inhibition of Ribosomal RNA Synthesis in E. coli by 2,4-Dinitrophenol
2,4-Dinitrophenol (DNP), when added to cultures of Escherichia coli in concentrations not greatly in excess of those required to inhibit growth, exhibits marked inhibition of RNA synthesis, while little effect on DNA and protein synthesis is seen. The selectivity disappears rapidly with increasing c...
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| Veröffentlicht in: | Molecular pharmacology 1966-01, Vol.2 (1), p.43-49 |
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| container_title | Molecular pharmacology |
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| creator | Simon, E J Van Praag, D Aronson, F L |
| description | 2,4-Dinitrophenol (DNP), when added to cultures of Escherichia coli in concentrations
not greatly in excess of those required to inhibit growth, exhibits marked inhibition
of RNA synthesis, while little effect on DNA and protein synthesis is seen. The selectivity disappears rapidly with increasing
concentrations of DNP. Evidence is presented
which demonstrates that low concentrations of DNP inhibit primarily the synthesis of
ribosomal RNA with relatively slight effects on the synthesis of messenger and transfer
RNAâs. At the concentrations at which selectivity is observed, neither oxidative phosphorylation nor transport of uracil across
the cell membrane appears to be affected
appreciably by DNP. The similarities and differences between the modes of action of
levorphanol and of DNP are discussed. |
| format | Article |
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not greatly in excess of those required to inhibit growth, exhibits marked inhibition
of RNA synthesis, while little effect on DNA and protein synthesis is seen. The selectivity disappears rapidly with increasing
concentrations of DNP. Evidence is presented
which demonstrates that low concentrations of DNP inhibit primarily the synthesis of
ribosomal RNA with relatively slight effects on the synthesis of messenger and transfer
RNAâs. At the concentrations at which selectivity is observed, neither oxidative phosphorylation nor transport of uracil across
the cell membrane appears to be affected
appreciably by DNP. The similarities and differences between the modes of action of
levorphanol and of DNP are discussed.</description><identifier>ISSN: 0026-895X</identifier><identifier>EISSN: 1521-0111</identifier><identifier>PMID: 5324993</identifier><language>eng</language><publisher>United States: American Society for Pharmacology and Experimental Therapeutics</publisher><subject>Dinitrophenols - pharmacology ; Escherichia coli - metabolism ; In Vitro Techniques ; Ribosomes - metabolism ; RNA, Bacterial - biosynthesis</subject><ispartof>Molecular pharmacology, 1966-01, Vol.2 (1), p.43-49</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5324993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simon, E J</creatorcontrib><creatorcontrib>Van Praag, D</creatorcontrib><creatorcontrib>Aronson, F L</creatorcontrib><title>The Selective Inhibition of Ribosomal RNA Synthesis in E. coli by 2,4-Dinitrophenol</title><title>Molecular pharmacology</title><addtitle>Mol Pharmacol</addtitle><description>2,4-Dinitrophenol (DNP), when added to cultures of Escherichia coli in concentrations
not greatly in excess of those required to inhibit growth, exhibits marked inhibition
of RNA synthesis, while little effect on DNA and protein synthesis is seen. The selectivity disappears rapidly with increasing
concentrations of DNP. Evidence is presented
which demonstrates that low concentrations of DNP inhibit primarily the synthesis of
ribosomal RNA with relatively slight effects on the synthesis of messenger and transfer
RNAâs. At the concentrations at which selectivity is observed, neither oxidative phosphorylation nor transport of uracil across
the cell membrane appears to be affected
appreciably by DNP. The similarities and differences between the modes of action of
levorphanol and of DNP are discussed.</description><subject>Dinitrophenols - pharmacology</subject><subject>Escherichia coli - metabolism</subject><subject>In Vitro Techniques</subject><subject>Ribosomes - metabolism</subject><subject>RNA, Bacterial - biosynthesis</subject><issn>0026-895X</issn><issn>1521-0111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1966</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkF9LwzAAxIMoc04_ghAQfLKSf23TxzE3HQyFbYJvIWnTJZImtemUfnsL29Nx3I-DuwswxSnBCcIYX4IpQiRLeJF-XYObGL8RwizlaAImKSWsKOgU7PZGw512uuztr4Zrb6yyvQ0ehhpurQoxNNLB7fsc7gbfGx1thNbD5TMsg7NQDZA8seTFett3oTXaB3cLrmrpor476wx8rpb7xVuy-XhdL-abxBDK-oQyTBnNy7TmVZEipBlTnHCFFJKjIVIRlJWK1JRJWTNUVbrKOWeVrHmuqKIz8Hjqbbvwc9SxF42NpXZOeh2OUXCGSZaTbATvz-BRNboSbWcb2Q3i_MKYP5xyYw_mz3ZatEZ2jRwXhsMgiMCCUfoPT8llNA</recordid><startdate>19660101</startdate><enddate>19660101</enddate><creator>Simon, E J</creator><creator>Van Praag, D</creator><creator>Aronson, F L</creator><general>American Society for Pharmacology and Experimental Therapeutics</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19660101</creationdate><title>The Selective Inhibition of Ribosomal RNA Synthesis in E. coli by 2,4-Dinitrophenol</title><author>Simon, E J ; Van Praag, D ; Aronson, F L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h234t-3413437c5f8d9500e44b828b0b0a0e42ab206cb2f34aaf40dded7884daf87b3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1966</creationdate><topic>Dinitrophenols - pharmacology</topic><topic>Escherichia coli - metabolism</topic><topic>In Vitro Techniques</topic><topic>Ribosomes - metabolism</topic><topic>RNA, Bacterial - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simon, E J</creatorcontrib><creatorcontrib>Van Praag, D</creatorcontrib><creatorcontrib>Aronson, F L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simon, E J</au><au>Van Praag, D</au><au>Aronson, F L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Selective Inhibition of Ribosomal RNA Synthesis in E. coli by 2,4-Dinitrophenol</atitle><jtitle>Molecular pharmacology</jtitle><addtitle>Mol Pharmacol</addtitle><date>1966-01-01</date><risdate>1966</risdate><volume>2</volume><issue>1</issue><spage>43</spage><epage>49</epage><pages>43-49</pages><issn>0026-895X</issn><eissn>1521-0111</eissn><abstract>2,4-Dinitrophenol (DNP), when added to cultures of Escherichia coli in concentrations
not greatly in excess of those required to inhibit growth, exhibits marked inhibition
of RNA synthesis, while little effect on DNA and protein synthesis is seen. The selectivity disappears rapidly with increasing
concentrations of DNP. Evidence is presented
which demonstrates that low concentrations of DNP inhibit primarily the synthesis of
ribosomal RNA with relatively slight effects on the synthesis of messenger and transfer
RNAâs. At the concentrations at which selectivity is observed, neither oxidative phosphorylation nor transport of uracil across
the cell membrane appears to be affected
appreciably by DNP. The similarities and differences between the modes of action of
levorphanol and of DNP are discussed.</abstract><cop>United States</cop><pub>American Society for Pharmacology and Experimental Therapeutics</pub><pmid>5324993</pmid><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Molecular pharmacology, 1966-01, Vol.2 (1), p.43-49 |
| issn | 0026-895X 1521-0111 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84126726 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Dinitrophenols - pharmacology Escherichia coli - metabolism In Vitro Techniques Ribosomes - metabolism RNA, Bacterial - biosynthesis |
| title | The Selective Inhibition of Ribosomal RNA Synthesis in E. coli by 2,4-Dinitrophenol |
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