A simplified method for production and growth of multicellular tumor spheroids

A new technique, based on the growth of tumor cells in liquid media over an agar base, has been developed for the formation and growth of multicellular tumor spheroids. All of the 11 transformed cell lines tested formed multicellular tumor spheroids, while none of the 8 normal cell types tested did...

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Veröffentlicht in:	Cancer research (Chicago, Ill.) Ill.), 1977-10, Vol.37 (10), p.3639-3643
Hauptverfasser:	Yuhas, J M, Li, A P, Martinez, A O, Ladman, A J
Format:	Artikel
Sprache:	eng
Schlagworte:	Agar Animals Cell Count Cell Division Cells, Cultured Cricetinae Culture Media Culture Techniques - methods Female Humans Kinetics Mice Mice, Inbred BALB C Mice, Inbred C3H Neoplasm Transplantation Neoplasms, Experimental - pathology Transplantation, Isogeneic
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description	A new technique, based on the growth of tumor cells in liquid media over an agar base, has been developed for the formation and growth of multicellular tumor spheroids. All of the 11 transformed cell lines tested formed multicellular tumor spheroids, while none of the 8 normal cell types tested did so. The advantages of the present technique over older methods include its simplicity, generality, and experimental flexibility.
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source	MEDLINE; American Association for Cancer Research; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects	Agar Animals Cell Count Cell Division Cells, Cultured Cricetinae Culture Media Culture Techniques - methods Female Humans Kinetics Mice Mice, Inbred BALB C Mice, Inbred C3H Neoplasm Transplantation Neoplasms, Experimental - pathology Transplantation, Isogeneic
title	A simplified method for production and growth of multicellular tumor spheroids
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